Experiment: MBOA 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3271 and Psyr_3272 are separated by 28 nucleotides Psyr_3272 and Psyr_3273 are separated by 13 nucleotides Psyr_3273 and Psyr_3274 are separated by 58 nucleotides
Psyr_3271: Psyr_3271 - methylmalonate-semialdehyde dehydrogenase (acylating), at 3,917,394 to 3,918,896
_3271
Psyr_3272: Psyr_3272 - 5-deoxyglucuronate isomerase, at 3,918,925 to 3,919,734
_3272
Psyr_3273: Psyr_3273 - 2-keto-myo-inositol dehydratase, at 3,919,748 to 3,920,653
_3273
Psyr_3274: Psyr_3274 - 5-dehydro-2-deoxygluconokinase, at 3,920,712 to 3,922,649
_3274
Position (kb)
3919
3920
3921 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3918.868 kb on + strand at 3918.868 kb on + strand at 3918.869 kb on - strand at 3918.994 kb on + strand at 3918.994 kb on + strand at 3918.994 kb on + strand at 3918.995 kb on - strand at 3919.004 kb on + strand at 3919.044 kb on - strand, within Psyr_3272 at 3919.161 kb on - strand, within Psyr_3272 at 3919.161 kb on - strand, within Psyr_3272 at 3919.161 kb on - strand, within Psyr_3272 at 3919.163 kb on + strand, within Psyr_3272 at 3919.163 kb on + strand, within Psyr_3272 at 3919.248 kb on + strand, within Psyr_3272 at 3919.248 kb on + strand, within Psyr_3272 at 3919.248 kb on + strand, within Psyr_3272 at 3919.248 kb on + strand, within Psyr_3272 at 3919.249 kb on - strand, within Psyr_3272 at 3919.297 kb on + strand, within Psyr_3272 at 3919.297 kb on + strand, within Psyr_3272 at 3919.297 kb on + strand, within Psyr_3272 at 3919.298 kb on - strand, within Psyr_3272 at 3919.298 kb on - strand, within Psyr_3272 at 3919.298 kb on - strand, within Psyr_3272 at 3919.298 kb on - strand, within Psyr_3272 at 3919.298 kb on - strand, within Psyr_3272 at 3919.298 kb on - strand, within Psyr_3272 at 3919.311 kb on - strand, within Psyr_3272 at 3919.311 kb on - strand, within Psyr_3272 at 3919.665 kb on - strand at 3919.785 kb on + strand at 3919.800 kb on + strand at 3919.800 kb on + strand at 3919.801 kb on - strand at 3919.801 kb on - strand at 3919.801 kb on - strand at 3919.801 kb on - strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.806 kb on + strand at 3919.807 kb on - strand at 3919.807 kb on - strand at 3919.807 kb on - strand at 3920.073 kb on + strand, within Psyr_3273 at 3920.073 kb on + strand, within Psyr_3273 at 3920.073 kb on + strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.074 kb on - strand, within Psyr_3273 at 3920.158 kb on - strand, within Psyr_3273 at 3920.325 kb on + strand, within Psyr_3273 at 3920.326 kb on - strand, within Psyr_3273 at 3920.462 kb on + strand at 3920.462 kb on + strand, within Psyr_3273 at 3920.462 kb on + strand, within Psyr_3273 at 3920.462 kb on + strand, within Psyr_3273 at 3920.463 kb on - strand, within Psyr_3273 at 3920.463 kb on - strand, within Psyr_3273 at 3920.463 kb on - strand, within Psyr_3273 at 3920.790 kb on + strand at 3920.829 kb on + strand at 3920.830 kb on - strand at 3920.928 kb on + strand at 3921.026 kb on - strand, within Psyr_3274 at 3921.040 kb on + strand, within Psyr_3274 at 3921.040 kb on + strand, within Psyr_3274 at 3921.306 kb on + strand, within Psyr_3274 at 3921.307 kb on - strand, within Psyr_3274
Per-strain Table
Position Strand Gene LocusTag Fraction MBOA 0.5 mM remove 3,918,868 + +0.8 3,918,868 + +0.2 3,918,869 - -0.3 3,918,994 + -0.7 3,918,994 + +0.1 3,918,994 + +0.6 3,918,995 - +0.1 3,919,004 + +1.3 3,919,044 - Psyr_3272 0.15 +0.5 3,919,161 - Psyr_3272 0.29 +0.0 3,919,161 - Psyr_3272 0.29 +0.2 3,919,161 - Psyr_3272 0.29 +1.3 3,919,163 + Psyr_3272 0.29 +0.6 3,919,163 + Psyr_3272 0.29 +0.1 3,919,248 + Psyr_3272 0.40 +0.6 3,919,248 + Psyr_3272 0.40 +0.1 3,919,248 + Psyr_3272 0.40 +1.3 3,919,248 + Psyr_3272 0.40 +0.4 3,919,249 - Psyr_3272 0.40 +0.7 3,919,297 + Psyr_3272 0.46 +1.7 3,919,297 + Psyr_3272 0.46 -0.1 3,919,297 + Psyr_3272 0.46 -1.3 3,919,298 - Psyr_3272 0.46 +0.4 3,919,298 - Psyr_3272 0.46 +1.1 3,919,298 - Psyr_3272 0.46 -2.0 3,919,298 - Psyr_3272 0.46 -0.0 3,919,298 - Psyr_3272 0.46 +1.2 3,919,298 - Psyr_3272 0.46 -0.1 3,919,311 - Psyr_3272 0.48 -0.2 3,919,311 - Psyr_3272 0.48 -0.4 3,919,665 - +0.8 3,919,785 + +1.2 3,919,800 + -0.8 3,919,800 + +0.9 3,919,801 - +0.5 3,919,801 - +1.0 3,919,801 - -1.3 3,919,801 - -0.5 3,919,806 + +0.5 3,919,806 + -0.0 3,919,806 + +0.7 3,919,806 + -0.2 3,919,806 + -0.0 3,919,806 + -0.0 3,919,806 + +0.7 3,919,806 + -1.5 3,919,806 + -1.3 3,919,806 + +0.2 3,919,807 - -1.1 3,919,807 - -0.5 3,919,807 - -1.1 3,920,073 + Psyr_3273 0.36 -0.2 3,920,073 + Psyr_3273 0.36 -0.6 3,920,073 + Psyr_3273 0.36 +0.8 3,920,074 - Psyr_3273 0.36 -0.5 3,920,074 - Psyr_3273 0.36 -0.8 3,920,074 - Psyr_3273 0.36 +0.2 3,920,074 - Psyr_3273 0.36 +0.4 3,920,074 - Psyr_3273 0.36 -1.2 3,920,074 - Psyr_3273 0.36 +0.3 3,920,074 - Psyr_3273 0.36 +0.4 3,920,074 - Psyr_3273 0.36 -1.6 3,920,074 - Psyr_3273 0.36 -0.1 3,920,158 - Psyr_3273 0.45 -3.8 3,920,325 + Psyr_3273 0.64 +0.1 3,920,326 - Psyr_3273 0.64 -0.9 3,920,462 + +1.2 3,920,462 + Psyr_3273 0.79 +0.2 3,920,462 + Psyr_3273 0.79 -0.5 3,920,462 + Psyr_3273 0.79 -1.3 3,920,463 - Psyr_3273 0.79 -0.5 3,920,463 - Psyr_3273 0.79 -0.9 3,920,463 - Psyr_3273 0.79 -0.7 3,920,790 + +0.3 3,920,829 + +0.0 3,920,830 - +0.4 3,920,928 + -0.1 3,921,026 - Psyr_3274 0.16 +0.2 3,921,040 + Psyr_3274 0.17 +0.5 3,921,040 + Psyr_3274 0.17 +0.1 3,921,306 + Psyr_3274 0.31 -0.6 3,921,307 - Psyr_3274 0.31 +0.1
Or see this region's nucleotide sequence