Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS01110

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS01100 and RS_RS01105 are separated by 266 nucleotidesRS_RS01105 and RS_RS01110 overlap by 4 nucleotidesRS_RS01110 and RS_RS01115 are separated by 88 nucleotidesRS_RS01115 and RS_RS01120 are separated by 42 nucleotides RS_RS01100: RS_RS01100 - sigma 54-dependent transcriptional regulator, at 243,896 to 245,503 _RS01100 RS_RS01105: RS_RS01105 - hypothetical protein, at 245,770 to 246,153 _RS01105 RS_RS01110: RS_RS01110 - RtcB family protein, at 246,150 to 247,370 _RS01110 RS_RS01115: RS_RS01115 - nucleotidyltransferase domain-containing protein, at 247,459 to 248,304 _RS01115 RS_RS01120: RS_RS01120 - RNA 3'-terminal phosphate cyclase, at 248,347 to 249,390 _RS01120 Position (kb) 246 247 248Strain fitness (log2 ratio) -1 0 1 2 3 4at 245.182 kb on - strand, within RS_RS01100at 245.208 kb on + strand, within RS_RS01100at 245.239 kb on - strand, within RS_RS01100at 245.515 kb on + strandat 245.525 kb on + strandat 245.525 kb on + strandat 245.678 kb on + strandat 245.679 kb on - strandat 245.929 kb on + strand, within RS_RS01105at 245.929 kb on + strand, within RS_RS01105at 245.930 kb on - strand, within RS_RS01105at 245.930 kb on - strand, within RS_RS01105at 245.959 kb on + strand, within RS_RS01105at 245.960 kb on - strand, within RS_RS01105at 245.962 kb on + strand, within RS_RS01105at 245.963 kb on - strand, within RS_RS01105at 245.963 kb on - strand, within RS_RS01105at 246.037 kb on + strand, within RS_RS01105at 246.038 kb on - strand, within RS_RS01105at 246.038 kb on - strand, within RS_RS01105at 246.055 kb on + strand, within RS_RS01105at 246.055 kb on + strand, within RS_RS01105at 246.165 kb on + strandat 246.165 kb on + strandat 246.165 kb on + strandat 246.166 kb on - strandat 246.166 kb on - strandat 246.597 kb on + strand, within RS_RS01110at 246.785 kb on + strand, within RS_RS01110at 246.817 kb on - strand, within RS_RS01110at 246.849 kb on + strand, within RS_RS01110at 246.850 kb on - strand, within RS_RS01110at 246.879 kb on + strand, within RS_RS01110at 246.879 kb on + strand, within RS_RS01110at 246.880 kb on - strand, within RS_RS01110at 246.880 kb on - strand, within RS_RS01110at 246.990 kb on + strand, within RS_RS01110at 246.990 kb on + strand, within RS_RS01110at 246.990 kb on + strand, within RS_RS01110at 246.990 kb on + strand, within RS_RS01110at 246.991 kb on - strand, within RS_RS01110at 246.991 kb on - strand, within RS_RS01110at 246.991 kb on - strand, within RS_RS01110at 246.991 kb on - strand, within RS_RS01110at 246.991 kb on - strand, within RS_RS01110at 247.197 kb on + strand, within RS_RS01110at 247.281 kb on + strandat 247.282 kb on - strandat 247.282 kb on - strandat 247.282 kb on - strandat 247.282 kb on - strandat 247.337 kb on + strandat 247.369 kb on - strandat 247.916 kb on - strand, within RS_RS01115at 247.982 kb on - strand, within RS_RS01115at 247.987 kb on + strand, within RS_RS01115at 247.987 kb on + strand, within RS_RS01115at 247.987 kb on + strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 248.149 kb on + strand, within RS_RS01115at 248.149 kb on + strand, within RS_RS01115at 248.149 kb on + strand, within RS_RS01115at 248.150 kb on - strand, within RS_RS01115at 248.266 kb on + strandat 248.267 kb on - strandat 248.267 kb on - strandat 248.337 kb on + strandat 248.337 kb on + strandat 248.337 kb on + strandat 248.360 kb on + strandat 248.361 kb on - strandat 248.361 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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245,182 - RS_RS01100 0.80 -1.1
245,208 + RS_RS01100 0.82 +1.2
245,239 - RS_RS01100 0.84 +0.2
245,515 + +0.2
245,525 + +0.3
245,525 + +0.8
245,678 + -0.1
245,679 - -0.1
245,929 + RS_RS01105 0.41 -0.1
245,929 + RS_RS01105 0.41 +1.1
245,930 - RS_RS01105 0.42 +2.2
245,930 - RS_RS01105 0.42 +0.1
245,959 + RS_RS01105 0.49 +0.5
245,960 - RS_RS01105 0.49 -0.3
245,962 + RS_RS01105 0.50 +2.2
245,963 - RS_RS01105 0.50 -0.1
245,963 - RS_RS01105 0.50 -0.2
246,037 + RS_RS01105 0.70 +1.2
246,038 - RS_RS01105 0.70 +0.6
246,038 - RS_RS01105 0.70 +0.2
246,055 + RS_RS01105 0.74 +0.3
246,055 + RS_RS01105 0.74 -1.4
246,165 + -0.5
246,165 + -0.6
246,165 + +1.0
246,166 - -0.2
246,166 - +0.3
246,597 + RS_RS01110 0.37 -0.6
246,785 + RS_RS01110 0.52 +1.0
246,817 - RS_RS01110 0.55 +0.2
246,849 + RS_RS01110 0.57 -0.4
246,850 - RS_RS01110 0.57 +2.2
246,879 + RS_RS01110 0.60 -0.8
246,879 + RS_RS01110 0.60 -0.1
246,880 - RS_RS01110 0.60 -0.0
246,880 - RS_RS01110 0.60 +0.7
246,990 + RS_RS01110 0.69 +0.2
246,990 + RS_RS01110 0.69 +1.5
246,990 + RS_RS01110 0.69 -0.8
246,990 + RS_RS01110 0.69 -0.3
246,991 - RS_RS01110 0.69 -0.4
246,991 - RS_RS01110 0.69 -1.1
246,991 - RS_RS01110 0.69 +0.4
246,991 - RS_RS01110 0.69 -0.4
246,991 - RS_RS01110 0.69 -0.5
247,197 + RS_RS01110 0.86 +1.0
247,281 + -1.3
247,282 - +2.2
247,282 - +0.1
247,282 - +0.4
247,282 - -0.6
247,337 + +2.2
247,369 - -0.2
247,916 - RS_RS01115 0.54 -0.2
247,982 - RS_RS01115 0.62 +1.3
247,987 + RS_RS01115 0.62 +0.6
247,987 + RS_RS01115 0.62 +0.3
247,987 + RS_RS01115 0.62 +0.2
247,988 - RS_RS01115 0.63 -1.6
247,988 - RS_RS01115 0.63 -0.8
247,988 - RS_RS01115 0.63 -0.0
247,988 - RS_RS01115 0.63 +0.7
247,988 - RS_RS01115 0.63 +4.1
247,988 - RS_RS01115 0.63 +1.2
247,988 - RS_RS01115 0.63 -1.6
248,149 + RS_RS01115 0.82 +1.2
248,149 + RS_RS01115 0.82 +0.6
248,149 + RS_RS01115 0.82 -0.6
248,150 - RS_RS01115 0.82 +1.3
248,266 + +0.6
248,267 - +0.2
248,267 - -1.1
248,337 + -0.6
248,337 + -0.1
248,337 + -1.1
248,360 + +2.2
248,361 - +0.6
248,361 - -0.8

Or see this region's nucleotide sequence