Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS00135 and RS_RS00140 are separated by 279 nucleotides RS_RS00140 and RS_RS00145 are separated by 4 nucleotides
RS_RS00135: RS_RS00135 - homoserine O-acetyltransferase, at 31,089 to 32,300
_RS00135
RS_RS00140: RS_RS00140 - NAD-dependent succinate-semialdehyde dehydrogenase, at 32,580 to 34,073
_RS00140
RS_RS00145: RS_RS00145 - 4-aminobutyrate--2-oxoglutarate transaminase, at 34,078 to 35,358
_RS00145
Position (kb) 32
33
34
35 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 31.600 kb on + strand, within RS_RS00135 at 31.600 kb on + strand, within RS_RS00135 at 31.600 kb on + strand, within RS_RS00135 at 31.601 kb on - strand, within RS_RS00135 at 31.601 kb on - strand, within RS_RS00135 at 31.604 kb on - strand, within RS_RS00135 at 31.604 kb on - strand, within RS_RS00135 at 31.729 kb on + strand, within RS_RS00135 at 31.730 kb on - strand, within RS_RS00135 at 31.730 kb on - strand, within RS_RS00135 at 31.780 kb on - strand, within RS_RS00135 at 31.873 kb on + strand, within RS_RS00135 at 32.107 kb on + strand, within RS_RS00135 at 32.108 kb on - strand, within RS_RS00135 at 32.108 kb on - strand, within RS_RS00135 at 32.108 kb on - strand, within RS_RS00135 at 32.414 kb on + strand at 32.414 kb on + strand at 32.510 kb on + strand at 32.510 kb on + strand at 32.512 kb on + strand at 32.512 kb on + strand at 32.512 kb on + strand at 32.512 kb on + strand at 32.512 kb on + strand at 32.513 kb on - strand at 32.513 kb on - strand at 32.513 kb on - strand at 32.513 kb on - strand at 32.605 kb on + strand at 32.606 kb on - strand at 32.620 kb on + strand at 32.635 kb on + strand at 32.635 kb on + strand at 32.636 kb on - strand at 32.779 kb on + strand, within RS_RS00140 at 32.780 kb on - strand, within RS_RS00140 at 32.780 kb on - strand, within RS_RS00140 at 32.786 kb on - strand, within RS_RS00140 at 33.139 kb on + strand, within RS_RS00140 at 33.139 kb on + strand, within RS_RS00140 at 33.139 kb on + strand, within RS_RS00140 at 33.139 kb on + strand, within RS_RS00140 at 33.140 kb on - strand, within RS_RS00140 at 33.140 kb on - strand, within RS_RS00140 at 33.140 kb on - strand, within RS_RS00140 at 33.157 kb on + strand, within RS_RS00140 at 33.158 kb on - strand, within RS_RS00140 at 33.484 kb on + strand, within RS_RS00140 at 33.484 kb on + strand, within RS_RS00140 at 33.485 kb on - strand, within RS_RS00140 at 33.485 kb on - strand, within RS_RS00140 at 33.665 kb on - strand, within RS_RS00140 at 34.123 kb on + strand at 34.173 kb on - strand at 34.173 kb on - strand at 34.275 kb on + strand, within RS_RS00145 at 34.275 kb on + strand, within RS_RS00145 at 34.451 kb on + strand, within RS_RS00145 at 34.451 kb on + strand, within RS_RS00145 at 34.451 kb on + strand, within RS_RS00145 at 34.452 kb on - strand, within RS_RS00145 at 34.456 kb on - strand, within RS_RS00145 at 34.700 kb on + strand, within RS_RS00145 at 34.700 kb on + strand, within RS_RS00145 at 34.701 kb on - strand, within RS_RS00145 at 34.844 kb on + strand, within RS_RS00145 at 34.845 kb on - strand, within RS_RS00145 at 34.845 kb on - strand, within RS_RS00145 at 34.845 kb on - strand, within RS_RS00145 at 34.845 kb on - strand, within RS_RS00145 at 34.880 kb on + strand, within RS_RS00145 at 34.880 kb on + strand, within RS_RS00145 at 34.880 kb on + strand, within RS_RS00145
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 31,600 + RS_RS00135 0.42 -0.1 31,600 + RS_RS00135 0.42 +2.2 31,600 + RS_RS00135 0.42 -1.0 31,601 - RS_RS00135 0.42 -3.2 31,601 - RS_RS00135 0.42 -6.6 31,604 - RS_RS00135 0.42 +1.2 31,604 - RS_RS00135 0.42 -1.8 31,729 + RS_RS00135 0.53 -2.9 31,730 - RS_RS00135 0.53 -4.6 31,730 - RS_RS00135 0.53 -3.2 31,780 - RS_RS00135 0.57 -0.4 31,873 + RS_RS00135 0.65 -1.7 32,107 + RS_RS00135 0.84 -0.1 32,108 - RS_RS00135 0.84 -2.4 32,108 - RS_RS00135 0.84 -1.7 32,108 - RS_RS00135 0.84 -0.8 32,414 + -0.8 32,414 + -0.4 32,510 + -5.6 32,510 + -0.6 32,512 + +1.2 32,512 + -0.2 32,512 + -3.1 32,512 + -1.8 32,512 + -0.1 32,513 - -2.0 32,513 - -0.4 32,513 - +1.6 32,513 - +0.8 32,605 + +0.2 32,606 - +2.2 32,620 + -0.1 32,635 + +2.2 32,635 + +0.6 32,636 - -0.6 32,779 + RS_RS00140 0.13 +0.6 32,780 - RS_RS00140 0.13 +2.2 32,780 - RS_RS00140 0.13 +0.9 32,786 - RS_RS00140 0.14 -1.4 33,139 + RS_RS00140 0.37 +1.2 33,139 + RS_RS00140 0.37 -0.9 33,139 + RS_RS00140 0.37 -0.1 33,139 + RS_RS00140 0.37 -0.4 33,140 - RS_RS00140 0.37 -0.4 33,140 - RS_RS00140 0.37 -1.4 33,140 - RS_RS00140 0.37 -0.3 33,157 + RS_RS00140 0.39 +0.7 33,158 - RS_RS00140 0.39 -0.4 33,484 + RS_RS00140 0.61 -2.0 33,484 + RS_RS00140 0.61 -0.1 33,485 - RS_RS00140 0.61 -1.3 33,485 - RS_RS00140 0.61 -1.6 33,665 - RS_RS00140 0.73 -1.6 34,123 + -2.9 34,173 - +0.6 34,173 - -1.7 34,275 + RS_RS00145 0.15 +0.0 34,275 + RS_RS00145 0.15 -0.9 34,451 + RS_RS00145 0.29 -0.2 34,451 + RS_RS00145 0.29 -0.3 34,451 + RS_RS00145 0.29 +0.2 34,452 - RS_RS00145 0.29 -0.2 34,456 - RS_RS00145 0.30 -2.2 34,700 + RS_RS00145 0.49 +2.2 34,700 + RS_RS00145 0.49 +2.2 34,701 - RS_RS00145 0.49 +2.2 34,844 + RS_RS00145 0.60 -1.4 34,845 - RS_RS00145 0.60 +1.3 34,845 - RS_RS00145 0.60 +0.1 34,845 - RS_RS00145 0.60 -0.4 34,845 - RS_RS00145 0.60 -0.2 34,880 + RS_RS00145 0.63 +0.6 34,880 + RS_RS00145 0.63 -0.1 34,880 + RS_RS00145 0.63 -1.4
Or see this region's nucleotide sequence