Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10400

Experiment: Sucrose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10395 and RS_RS10400 are separated by 340 nucleotidesRS_RS10400 and RS_RS10405 are separated by 13 nucleotidesRS_RS10405 and RS_RS10410 are separated by 148 nucleotides RS_RS10395: RS_RS10395 - 2-isopropylmalate synthase, at 2,244,697 to 2,246,238 _RS10395 RS_RS10400: RS_RS10400 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 2,246,579 to 2,247,454 _RS10400 RS_RS10405: RS_RS10405 - phosphatidylserine decarboxylase proenzyme, at 2,247,468 to 2,248,115 _RS10405 RS_RS10410: RS_RS10410 - ketol-acid reductoisomerase, at 2,248,264 to 2,249,280 _RS10410 Position (kb) 2246 2247 2248Strain fitness (log2 ratio) -1 0 1at 2245.701 kb on - strand, within RS_RS10395at 2245.883 kb on + strand, within RS_RS10395at 2245.921 kb on - strand, within RS_RS10395at 2245.921 kb on - strand, within RS_RS10395at 2246.413 kb on + strandat 2248.158 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sucrose (C)
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2,245,701 - RS_RS10395 0.65 -1.5
2,245,883 + RS_RS10395 0.77 +0.7
2,245,921 - RS_RS10395 0.79 +0.7
2,245,921 - RS_RS10395 0.79 -0.5
2,246,413 + +0.7
2,248,158 - +1.0

Or see this region's nucleotide sequence