Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS18305

Experiment: D-Galactose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS18300 and RS_RS26020 are separated by 65 nucleotidesRS_RS26020 and RS_RS18305 are separated by 329 nucleotidesRS_RS18305 and RS_RS18310 overlap by 4 nucleotides RS_RS18300: RS_RS18300 - glycogen debranching enzyme GlgX, at 300,326 to 302,590 _RS18300 RS_RS26020: RS_RS26020 - hypothetical protein, at 302,656 to 302,853 _RS26020 RS_RS18305: RS_RS18305 - glycogen-branching enzyme, at 303,183 to 305,510 _RS18305 RS_RS18310: RS_RS18310 - maltose alpha-D-glucosyltransferase, at 305,507 to 308,989 _RS18310 Position (kb) 303 304 305 306Strain fitness (log2 ratio) -1 0 1 2at 302.286 kb on + strand, within RS_RS18300at 302.287 kb on - strand, within RS_RS18300at 302.332 kb on - strand, within RS_RS18300at 302.365 kb on - strandat 302.365 kb on - strandat 302.395 kb on - strandat 302.543 kb on + strandat 302.551 kb on - strandat 302.551 kb on - strandat 302.585 kb on + strandat 303.264 kb on + strandat 303.265 kb on - strandat 303.265 kb on - strandat 303.265 kb on - strandat 303.448 kb on + strand, within RS_RS18305at 303.541 kb on + strand, within RS_RS18305at 303.542 kb on - strand, within RS_RS18305at 303.868 kb on + strand, within RS_RS18305at 303.889 kb on + strand, within RS_RS18305at 303.889 kb on + strand, within RS_RS18305at 303.889 kb on + strand, within RS_RS18305at 303.890 kb on - strand, within RS_RS18305at 303.890 kb on - strand, within RS_RS18305at 303.890 kb on - strand, within RS_RS18305at 303.890 kb on - strand, within RS_RS18305at 303.928 kb on + strand, within RS_RS18305at 303.928 kb on + strand, within RS_RS18305at 303.928 kb on + strand, within RS_RS18305at 303.928 kb on + strand, within RS_RS18305at 303.928 kb on + strand, within RS_RS18305at 303.928 kb on + strand, within RS_RS18305at 304.121 kb on - strand, within RS_RS18305at 304.121 kb on - strand, within RS_RS18305at 304.240 kb on + strand, within RS_RS18305at 304.241 kb on - strand, within RS_RS18305at 304.241 kb on - strand, within RS_RS18305at 304.241 kb on - strand, within RS_RS18305at 304.295 kb on - strand, within RS_RS18305at 304.295 kb on - strand, within RS_RS18305at 304.466 kb on - strand, within RS_RS18305at 304.540 kb on + strand, within RS_RS18305at 304.541 kb on - strand, within RS_RS18305at 304.630 kb on + strand, within RS_RS18305at 304.630 kb on + strand, within RS_RS18305at 304.817 kb on - strand, within RS_RS18305at 304.817 kb on - strand, within RS_RS18305at 304.817 kb on - strand, within RS_RS18305at 305.634 kb on + strandat 305.634 kb on + strandat 305.634 kb on + strandat 305.634 kb on + strandat 305.634 kb on + strandat 305.635 kb on - strandat 305.635 kb on - strandat 305.635 kb on - strandat 305.646 kb on + strandat 305.739 kb on + strandat 305.739 kb on + strandat 305.856 kb on + strand, within RS_RS18310at 305.857 kb on - strand, within RS_RS18310at 305.959 kb on - strand, within RS_RS18310at 305.959 kb on - strand, within RS_RS18310at 305.959 kb on - strand, within RS_RS18310

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galactose (C)
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302,286 + RS_RS18300 0.87 -1.0
302,287 - RS_RS18300 0.87 -0.5
302,332 - RS_RS18300 0.89 -0.2
302,365 - -0.1
302,365 - -0.4
302,395 - -0.1
302,543 + -0.5
302,551 - +1.1
302,551 - -0.5
302,585 + +0.2
303,264 + +0.4
303,265 - -0.2
303,265 - +0.5
303,265 - -0.1
303,448 + RS_RS18305 0.11 +0.5
303,541 + RS_RS18305 0.15 -1.3
303,542 - RS_RS18305 0.15 -0.5
303,868 + RS_RS18305 0.29 -0.5
303,889 + RS_RS18305 0.30 -0.5
303,889 + RS_RS18305 0.30 +2.1
303,889 + RS_RS18305 0.30 +0.1
303,890 - RS_RS18305 0.30 +0.1
303,890 - RS_RS18305 0.30 +0.2
303,890 - RS_RS18305 0.30 +0.3
303,890 - RS_RS18305 0.30 -0.8
303,928 + RS_RS18305 0.32 -0.5
303,928 + RS_RS18305 0.32 -0.7
303,928 + RS_RS18305 0.32 +2.1
303,928 + RS_RS18305 0.32 +1.1
303,928 + RS_RS18305 0.32 +0.0
303,928 + RS_RS18305 0.32 +0.3
304,121 - RS_RS18305 0.40 +0.1
304,121 - RS_RS18305 0.40 -1.1
304,240 + RS_RS18305 0.45 +2.1
304,241 - RS_RS18305 0.45 +2.1
304,241 - RS_RS18305 0.45 +0.2
304,241 - RS_RS18305 0.45 +0.4
304,295 - RS_RS18305 0.48 -0.7
304,295 - RS_RS18305 0.48 +2.1
304,466 - RS_RS18305 0.55 +2.1
304,540 + RS_RS18305 0.58 -0.7
304,541 - RS_RS18305 0.58 +0.5
304,630 + RS_RS18305 0.62 -0.1
304,630 + RS_RS18305 0.62 +1.1
304,817 - RS_RS18305 0.70 -0.2
304,817 - RS_RS18305 0.70 -0.2
304,817 - RS_RS18305 0.70 +2.1
305,634 + +0.2
305,634 + -0.0
305,634 + -0.5
305,634 + -1.1
305,634 + +0.1
305,635 - +0.1
305,635 - +0.1
305,635 - +1.1
305,646 + -0.9
305,739 + -0.3
305,739 + +0.5
305,856 + RS_RS18310 0.10 -0.6
305,857 - RS_RS18310 0.10 -0.5
305,959 - RS_RS18310 0.13 +0.8
305,959 - RS_RS18310 0.13 +0.2
305,959 - RS_RS18310 0.13 +0.8

Or see this region's nucleotide sequence