Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS12230

Experiment: D-Galactose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS12225 and RS_RS12230 are separated by 71 nucleotidesRS_RS12230 and RS_RS12235 are separated by 57 nucleotidesRS_RS12235 and RS_RS12240 are separated by 27 nucleotides RS_RS12225: RS_RS12225 - aspartate aminotransferase family protein, at 2,641,357 to 2,642,556 _RS12225 RS_RS12230: RS_RS12230 - N-acetyltransferase, at 2,642,628 to 2,643,125 _RS12230 RS_RS12235: RS_RS12235 - ABC transporter ATP-binding protein, at 2,643,183 to 2,643,899 _RS12235 RS_RS12240: RS_RS12240 - ABC transporter ATP-binding protein, at 2,643,927 to 2,644,700 _RS12240 Position (kb) 2642 2643 2644Strain fitness (log2 ratio) -1 0 1 2at 2642.707 kb on - strand, within RS_RS12230at 2642.731 kb on - strand, within RS_RS12230at 2642.878 kb on - strand, within RS_RS12230at 2643.015 kb on + strand, within RS_RS12230at 2643.084 kb on + strandat 2643.085 kb on - strandat 2643.196 kb on + strandat 2643.709 kb on + strand, within RS_RS12235at 2643.710 kb on - strand, within RS_RS12235at 2643.710 kb on - strand, within RS_RS12235at 2643.710 kb on - strand, within RS_RS12235at 2643.885 kb on + strandat 2643.886 kb on - strandat 2643.886 kb on - strandat 2643.949 kb on + strandat 2643.950 kb on - strandat 2643.950 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galactose (C)
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2,642,707 - RS_RS12230 0.16 -0.2
2,642,731 - RS_RS12230 0.21 -1.1
2,642,878 - RS_RS12230 0.50 -0.3
2,643,015 + RS_RS12230 0.78 -0.0
2,643,084 + +0.6
2,643,085 - -0.9
2,643,196 + -0.0
2,643,709 + RS_RS12235 0.73 +2.0
2,643,710 - RS_RS12235 0.74 -1.1
2,643,710 - RS_RS12235 0.74 +0.4
2,643,710 - RS_RS12235 0.74 +1.5
2,643,885 + +0.9
2,643,886 - -1.3
2,643,886 - +2.0
2,643,949 + +1.0
2,643,950 - -0.1
2,643,950 - +0.4

Or see this region's nucleotide sequence