Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS04585 and RS_RS04590 are separated by 177 nucleotides RS_RS04590 and RS_RS04595 are separated by 54 nucleotides RS_RS04595 and RS_RS04600 are separated by 98 nucleotides
RS_RS04585: RS_RS04585 - MetQ/NlpA family ABC transporter substrate-binding protein, at 973,512 to 974,312
_RS04585
RS_RS04590: RS_RS04590 - electron transfer flavoprotein subunit beta/FixA family protein, at 974,490 to 975,239
_RS04590
RS_RS04595: RS_RS04595 - electron transfer flavoprotein subunit alpha, at 975,294 to 976,229
_RS04595
RS_RS04600: RS_RS04600 - acyl-CoA dehydrogenase, at 976,328 to 978,112
_RS04600
Position (kb) 975
976
977 Strain fitness (log2 ratio) -2
-1
0
1 at 974.339 kb on + strand at 974.339 kb on + strand at 974.340 kb on - strand at 974.364 kb on + strand at 974.364 kb on + strand at 974.365 kb on - strand at 974.365 kb on - strand at 974.440 kb on - strand at 976.308 kb on + strand at 976.334 kb on + strand at 976.334 kb on + strand at 976.334 kb on + strand at 976.335 kb on - strand at 976.335 kb on - strand at 976.335 kb on - strand at 976.335 kb on - strand at 976.769 kb on + strand, within RS_RS04600 at 976.769 kb on + strand, within RS_RS04600 at 976.769 kb on + strand, within RS_RS04600 at 976.769 kb on + strand, within RS_RS04600 at 976.911 kb on - strand, within RS_RS04600 at 977.189 kb on + strand, within RS_RS04600 at 977.189 kb on + strand, within RS_RS04600 at 977.189 kb on + strand, within RS_RS04600
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 974,339 + +1.5 974,339 + +0.5 974,340 - -0.1 974,364 + -0.1 974,364 + +0.2 974,365 - +0.5 974,365 - -0.1 974,440 - -0.8 976,308 + -0.6 976,334 + +0.6 976,334 + +0.2 976,334 + +0.5 976,335 - +0.3 976,335 - -0.1 976,335 - +0.1 976,335 - -1.7 976,769 + RS_RS04600 0.25 -0.3 976,769 + RS_RS04600 0.25 +0.5 976,769 + RS_RS04600 0.25 -0.1 976,769 + RS_RS04600 0.25 -0.3 976,911 - RS_RS04600 0.33 -0.5 977,189 + RS_RS04600 0.48 +1.7 977,189 + RS_RS04600 0.48 -1.2 977,189 + RS_RS04600 0.48 -2.1
Or see this region's nucleotide sequence