Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS01325 and RS_RS01330 overlap by 4 nucleotides RS_RS01330 and RS_RS01335 overlap by 4 nucleotides RS_RS01335 and RS_RS01340 are separated by 24 nucleotides RS_RS01340 and RS_RS01345 are separated by 43 nucleotides
RS_RS01325: RS_RS01325 - hypothetical protein, at 303,723 to 304,157
_RS01325
RS_RS01330: RS_RS01330 - HAD family phosphatase, at 304,154 to 304,843
_RS01330
RS_RS01335: RS_RS01335 - rhodanese-like domain-containing protein, at 304,840 to 305,265
_RS01335
RS_RS01340: RS_RS01340 - 2-hydroxychromene-2-carboxylate isomerase, at 305,290 to 305,895
_RS01340
RS_RS01345: RS_RS01345 - butyryl-CoA dehydrogenase, at 305,939 to 307,069
_RS01345
Position (kb) 304
305
306 Strain fitness (log2 ratio) -2
-1
0
1
2 at 303.841 kb on + strand, within RS_RS01325 at 303.841 kb on + strand, within RS_RS01325 at 303.992 kb on - strand, within RS_RS01325 at 304.012 kb on + strand, within RS_RS01325 at 304.012 kb on + strand, within RS_RS01325 at 304.012 kb on + strand, within RS_RS01325 at 304.013 kb on - strand, within RS_RS01325 at 304.013 kb on - strand, within RS_RS01325 at 304.021 kb on + strand, within RS_RS01325 at 304.022 kb on - strand, within RS_RS01325 at 304.022 kb on - strand, within RS_RS01325 at 304.417 kb on + strand, within RS_RS01330 at 304.418 kb on - strand, within RS_RS01330 at 305.288 kb on + strand at 305.289 kb on - strand at 305.291 kb on + strand at 305.291 kb on + strand at 305.291 kb on + strand at 305.292 kb on - strand at 305.292 kb on - strand at 305.292 kb on - strand at 305.292 kb on - strand at 305.627 kb on + strand, within RS_RS01340 at 305.807 kb on + strand, within RS_RS01340 at 305.807 kb on + strand, within RS_RS01340 at 305.808 kb on - strand, within RS_RS01340 at 305.808 kb on - strand, within RS_RS01340 at 305.808 kb on - strand, within RS_RS01340 at 305.873 kb on + strand at 305.991 kb on + strand at 305.991 kb on + strand at 305.991 kb on + strand at 305.991 kb on + strand at 305.991 kb on + strand at 305.992 kb on - strand at 305.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 303,841 + RS_RS01325 0.27 +0.8 303,841 + RS_RS01325 0.27 +1.8 303,992 - RS_RS01325 0.62 -0.7 304,012 + RS_RS01325 0.66 -0.1 304,012 + RS_RS01325 0.66 -0.2 304,012 + RS_RS01325 0.66 +0.5 304,013 - RS_RS01325 0.67 -0.8 304,013 - RS_RS01325 0.67 +0.8 304,021 + RS_RS01325 0.69 +0.1 304,022 - RS_RS01325 0.69 +1.8 304,022 - RS_RS01325 0.69 +0.9 304,417 + RS_RS01330 0.38 +0.1 304,418 - RS_RS01330 0.38 +0.2 305,288 + -1.0 305,289 - -0.6 305,291 + -0.0 305,291 + -2.2 305,291 + +0.5 305,292 - -1.4 305,292 - -0.6 305,292 - -1.0 305,292 - -1.4 305,627 + RS_RS01340 0.56 +0.7 305,807 + RS_RS01340 0.85 -0.2 305,807 + RS_RS01340 0.85 -0.8 305,808 - RS_RS01340 0.85 +1.2 305,808 - RS_RS01340 0.85 -0.6 305,808 - RS_RS01340 0.85 -1.6 305,873 + -1.2 305,991 + -0.9 305,991 + +0.0 305,991 + -1.2 305,991 + +0.4 305,991 + -0.6 305,992 - -1.0 305,992 - -0.6
Or see this region's nucleotide sequence