Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10385

Experiment: L-Aspartic Acid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10375 and RS_RS10380 are separated by 307 nucleotidesRS_RS10380 and RS_RS10385 are separated by 161 nucleotidesRS_RS10385 and RS_RS10390 are separated by 97 nucleotidesRS_RS10390 and RS_RS10395 are separated by 245 nucleotides RS_RS10375: RS_RS10375 - polyribonucleotide nucleotidyltransferase, at 2,239,684 to 2,241,837 _RS10375 RS_RS10380: RS_RS10380 - 30S ribosomal protein S15, at 2,242,145 to 2,242,414 _RS10380 RS_RS10385: RS_RS10385 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,242,576 to 2,243,832 _RS10385 RS_RS10390: RS_RS10390 - membrane protein, at 2,243,930 to 2,244,451 _RS10390 RS_RS10395: RS_RS10395 - 2-isopropylmalate synthase, at 2,244,697 to 2,246,238 _RS10395 Position (kb) 2242 2243 2244Strain fitness (log2 ratio) -1 0 1at 2242.581 kb on - strandat 2243.039 kb on + strand, within RS_RS10385at 2243.039 kb on + strand, within RS_RS10385at 2243.040 kb on - strand, within RS_RS10385at 2243.163 kb on - strand, within RS_RS10385at 2243.282 kb on + strand, within RS_RS10385at 2243.282 kb on + strand, within RS_RS10385at 2243.283 kb on - strand, within RS_RS10385at 2243.301 kb on - strand, within RS_RS10385at 2243.832 kb on + strandat 2243.832 kb on + strandat 2243.860 kb on + strandat 2243.861 kb on - strandat 2244.290 kb on + strand, within RS_RS10390at 2244.423 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Aspartic Acid (C)
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2,242,581 - +0.1
2,243,039 + RS_RS10385 0.37 +0.6
2,243,039 + RS_RS10385 0.37 +0.4
2,243,040 - RS_RS10385 0.37 +0.1
2,243,163 - RS_RS10385 0.47 -1.7
2,243,282 + RS_RS10385 0.56 +0.0
2,243,282 + RS_RS10385 0.56 +0.3
2,243,283 - RS_RS10385 0.56 +0.2
2,243,301 - RS_RS10385 0.58 +0.4
2,243,832 + +0.0
2,243,832 + -0.7
2,243,860 + +0.2
2,243,861 - -0.3
2,244,290 + RS_RS10390 0.69 -0.7
2,244,423 - -0.1

Or see this region's nucleotide sequence