Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS14385

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS14380 and RS_RS14385 are separated by 602 nucleotidesRS_RS14385 and RS_RS14390 are separated by 79 nucleotidesRS_RS14390 and RS_RS14395 overlap by 4 nucleotides RS_RS14380: RS_RS14380 - 16S ribosomal RNA, at 3,099,489 to 3,101,033 _RS14380 RS_RS14385: RS_RS14385 - enoyl-CoA hydratase, at 3,101,636 to 3,102,412 _RS14385 RS_RS14390: RS_RS14390 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 3,102,492 to 3,103,280 _RS14390 RS_RS14395: RS_RS14395 - phenylacetic acid degradation protein PaaD, at 3,103,277 to 3,103,744 _RS14395 Position (kb) 3101 3102 3103Strain fitness (log2 ratio) -1 0 1 2at 3102.165 kb on + strand, within RS_RS14385at 3102.166 kb on - strand, within RS_RS14385at 3102.166 kb on - strand, within RS_RS14385at 3102.175 kb on + strand, within RS_RS14385at 3102.406 kb on - strandat 3102.406 kb on - strandat 3102.406 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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3,102,165 + RS_RS14385 0.68 +0.4
3,102,166 - RS_RS14385 0.68 +1.5
3,102,166 - RS_RS14385 0.68 -1.4
3,102,175 + RS_RS14385 0.69 +0.3
3,102,406 - -0.0
3,102,406 - +2.4
3,102,406 - -0.2

Or see this region's nucleotide sequence