Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10115

Experiment: L-Glutamine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10105 and RS_RS10110 are separated by 12 nucleotidesRS_RS10110 and RS_RS10115 are separated by 101 nucleotidesRS_RS10115 and RS_RS10120 are separated by 30 nucleotidesRS_RS10120 and RS_RS10125 are separated by 11 nucleotides RS_RS10105: RS_RS10105 - pyridoxal phosphate-dependent aminotransferase, at 2,189,763 to 2,190,962 _RS10105 RS_RS10110: RS_RS10110 - glutathione S-transferase, at 2,190,975 to 2,191,631 _RS10110 RS_RS10115: RS_RS10115 - 3-hydroxyacyl-CoA dehydrogenase, at 2,191,733 to 2,193,241 _RS10115 RS_RS10120: RS_RS10120 - enoyl-CoA hydratase, at 2,193,272 to 2,194,051 _RS10120 RS_RS10125: RS_RS10125 - histidine phosphatase family protein, at 2,194,063 to 2,194,797 _RS10125 Position (kb) 2191 2192 2193 2194Strain fitness (log2 ratio) -2 -1 0 1 2at 2190.760 kb on - strand, within RS_RS10105at 2190.778 kb on - strand, within RS_RS10105at 2190.867 kb on + strandat 2190.868 kb on - strandat 2190.868 kb on - strandat 2190.960 kb on + strandat 2190.961 kb on - strandat 2190.994 kb on + strandat 2190.995 kb on - strandat 2191.120 kb on + strand, within RS_RS10110at 2191.120 kb on + strand, within RS_RS10110at 2191.204 kb on + strand, within RS_RS10110at 2191.204 kb on + strand, within RS_RS10110at 2191.205 kb on - strand, within RS_RS10110at 2191.205 kb on - strand, within RS_RS10110at 2191.205 kb on - strand, within RS_RS10110at 2191.205 kb on - strand, within RS_RS10110at 2191.435 kb on + strand, within RS_RS10110at 2191.435 kb on + strand, within RS_RS10110at 2191.435 kb on + strand, within RS_RS10110at 2191.435 kb on + strand, within RS_RS10110at 2191.486 kb on + strand, within RS_RS10110at 2191.486 kb on + strand, within RS_RS10110at 2191.486 kb on + strand, within RS_RS10110at 2191.486 kb on + strand, within RS_RS10110at 2191.487 kb on - strand, within RS_RS10110at 2191.487 kb on - strand, within RS_RS10110at 2191.487 kb on - strand, within RS_RS10110at 2191.487 kb on - strand, within RS_RS10110at 2191.487 kb on - strand, within RS_RS10110at 2191.595 kb on - strandat 2191.597 kb on + strandat 2191.598 kb on - strandat 2191.598 kb on - strandat 2191.598 kb on - strandat 2191.618 kb on + strandat 2191.618 kb on + strandat 2191.618 kb on + strandat 2191.649 kb on + strandat 2191.650 kb on - strandat 2191.870 kb on + strandat 2192.471 kb on - strand, within RS_RS10115at 2192.480 kb on + strand, within RS_RS10115at 2192.481 kb on - strand, within RS_RS10115at 2192.835 kb on - strand, within RS_RS10115at 2193.365 kb on + strand, within RS_RS10120at 2193.365 kb on + strand, within RS_RS10120at 2193.365 kb on + strand, within RS_RS10120at 2193.365 kb on + strand, within RS_RS10120at 2193.675 kb on - strand, within RS_RS10120at 2194.126 kb on + strandat 2194.127 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (C)
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2,190,760 - RS_RS10105 0.83 -0.1
2,190,778 - RS_RS10105 0.85 +0.9
2,190,867 + -1.5
2,190,868 - -0.1
2,190,868 - -1.6
2,190,960 + +0.0
2,190,961 - -2.3
2,190,994 + -0.6
2,190,995 - +0.9
2,191,120 + RS_RS10110 0.22 +0.4
2,191,120 + RS_RS10110 0.22 -0.2
2,191,204 + RS_RS10110 0.35 -1.1
2,191,204 + RS_RS10110 0.35 -0.9
2,191,205 - RS_RS10110 0.35 -1.2
2,191,205 - RS_RS10110 0.35 +1.9
2,191,205 - RS_RS10110 0.35 -0.1
2,191,205 - RS_RS10110 0.35 -0.4
2,191,435 + RS_RS10110 0.70 +2.4
2,191,435 + RS_RS10110 0.70 +0.9
2,191,435 + RS_RS10110 0.70 -0.1
2,191,435 + RS_RS10110 0.70 -1.4
2,191,486 + RS_RS10110 0.78 -0.4
2,191,486 + RS_RS10110 0.78 -0.6
2,191,486 + RS_RS10110 0.78 +1.0
2,191,486 + RS_RS10110 0.78 +0.4
2,191,487 - RS_RS10110 0.78 -0.0
2,191,487 - RS_RS10110 0.78 -0.4
2,191,487 - RS_RS10110 0.78 +0.9
2,191,487 - RS_RS10110 0.78 -0.3
2,191,487 - RS_RS10110 0.78 -1.1
2,191,595 - +0.1
2,191,597 + -0.6
2,191,598 - -0.9
2,191,598 - -1.2
2,191,598 - +1.8
2,191,618 + +0.6
2,191,618 + -2.6
2,191,618 + +0.1
2,191,649 + +0.2
2,191,650 - -0.6
2,191,870 + -0.4
2,192,471 - RS_RS10115 0.49 -0.6
2,192,480 + RS_RS10115 0.50 +1.1
2,192,481 - RS_RS10115 0.50 +0.6
2,192,835 - RS_RS10115 0.73 -0.1
2,193,365 + RS_RS10120 0.12 +0.4
2,193,365 + RS_RS10120 0.12 +0.1
2,193,365 + RS_RS10120 0.12 -0.9
2,193,365 + RS_RS10120 0.12 -0.2
2,193,675 - RS_RS10120 0.52 -1.6
2,194,126 + -0.3
2,194,127 - +1.8

Or see this region's nucleotide sequence