Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS02175 and RS_RS02180 are separated by 69 nucleotides RS_RS02180 and RS_RS02185 are separated by 35 nucleotides RS_RS02185 and RS_RS02190 are separated by 230 nucleotides
RS_RS02175: RS_RS02175 - alpha/beta hydrolase, at 466,349 to 467,302
_RS02175
RS_RS02180: RS_RS02180 - hypothetical protein, at 467,372 to 468,175
_RS02180
RS_RS02185: RS_RS02185 - gluconate kinase, at 468,211 to 468,720
_RS02185
RS_RS02190: RS_RS02190 - AMP-binding protein, at 468,951 to 470,666
_RS02190
Position (kb) 467
468
469 Strain fitness (log2 ratio) -1
0
1
2 at 466.586 kb on + strand, within RS_RS02175 at 466.587 kb on - strand, within RS_RS02175 at 466.989 kb on - strand, within RS_RS02175 at 467.039 kb on + strand, within RS_RS02175 at 467.075 kb on + strand, within RS_RS02175 at 467.075 kb on + strand, within RS_RS02175 at 467.076 kb on - strand, within RS_RS02175 at 467.076 kb on - strand, within RS_RS02175 at 467.209 kb on - strand at 467.549 kb on + strand, within RS_RS02180 at 467.549 kb on + strand, within RS_RS02180 at 467.549 kb on + strand, within RS_RS02180 at 467.705 kb on + strand, within RS_RS02180 at 467.706 kb on - strand, within RS_RS02180 at 467.864 kb on + strand, within RS_RS02180 at 467.964 kb on - strand, within RS_RS02180 at 467.978 kb on + strand, within RS_RS02180 at 467.978 kb on + strand, within RS_RS02180 at 468.530 kb on + strand, within RS_RS02185 at 468.530 kb on + strand, within RS_RS02185 at 468.531 kb on - strand, within RS_RS02185 at 468.755 kb on + strand at 468.755 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 466,586 + RS_RS02175 0.25 +1.2 466,587 - RS_RS02175 0.25 +0.1 466,989 - RS_RS02175 0.67 +0.2 467,039 + RS_RS02175 0.72 -0.4 467,075 + RS_RS02175 0.76 +1.2 467,075 + RS_RS02175 0.76 +1.8 467,076 - RS_RS02175 0.76 +0.3 467,076 - RS_RS02175 0.76 -1.6 467,209 - +0.6 467,549 + RS_RS02180 0.22 -0.6 467,549 + RS_RS02180 0.22 -0.6 467,549 + RS_RS02180 0.22 +0.4 467,705 + RS_RS02180 0.41 -0.1 467,706 - RS_RS02180 0.42 -0.6 467,864 + RS_RS02180 0.61 -1.1 467,964 - RS_RS02180 0.74 -0.2 467,978 + RS_RS02180 0.75 +2.2 467,978 + RS_RS02180 0.75 +0.5 468,530 + RS_RS02185 0.63 +0.2 468,530 + RS_RS02185 0.63 -1.7 468,531 - RS_RS02185 0.63 -0.9 468,755 + +0.5 468,755 + -0.4
Or see this region's nucleotide sequence