Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS01120

Experiment: D-Galactose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS01110 and RS_RS01115 are separated by 88 nucleotidesRS_RS01115 and RS_RS01120 are separated by 42 nucleotidesRS_RS01120 and RS_RS25270 are separated by 682 nucleotides RS_RS01110: RS_RS01110 - RtcB family protein, at 246,150 to 247,370 _RS01110 RS_RS01115: RS_RS01115 - nucleotidyltransferase domain-containing protein, at 247,459 to 248,304 _RS01115 RS_RS01120: RS_RS01120 - RNA 3'-terminal phosphate cyclase, at 248,347 to 249,390 _RS01120 RS_RS25270: RS_RS25270 - hypothetical protein, at 250,073 to 250,351 _RS25270 Position (kb) 248 249 250Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 247.369 kb on - strandat 247.916 kb on - strand, within RS_RS01115at 247.982 kb on - strand, within RS_RS01115at 247.987 kb on + strand, within RS_RS01115at 247.987 kb on + strand, within RS_RS01115at 247.987 kb on + strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 247.988 kb on - strand, within RS_RS01115at 248.149 kb on + strand, within RS_RS01115at 248.149 kb on + strand, within RS_RS01115at 248.149 kb on + strand, within RS_RS01115at 248.150 kb on - strand, within RS_RS01115at 248.266 kb on + strandat 248.267 kb on - strandat 248.267 kb on - strandat 248.337 kb on + strandat 248.337 kb on + strandat 248.337 kb on + strandat 248.360 kb on + strandat 248.361 kb on - strandat 248.361 kb on - strandat 248.561 kb on + strand, within RS_RS01120at 248.627 kb on - strand, within RS_RS01120at 248.842 kb on + strand, within RS_RS01120at 249.653 kb on + strandat 249.654 kb on - strandat 249.654 kb on - strandat 249.656 kb on - strandat 249.874 kb on - strandat 249.874 kb on - strandat 249.953 kb on + strandat 249.978 kb on + strandat 250.032 kb on + strandat 250.032 kb on + strandat 250.032 kb on + strandat 250.033 kb on - strandat 250.033 kb on - strandat 250.114 kb on + strand, within RS_RS25270at 250.115 kb on - strand, within RS_RS25270at 250.119 kb on + strand, within RS_RS25270at 250.166 kb on - strand, within RS_RS25270at 250.166 kb on - strand, within RS_RS25270at 250.240 kb on + strand, within RS_RS25270at 250.240 kb on + strand, within RS_RS25270at 250.240 kb on + strand, within RS_RS25270at 250.241 kb on - strand, within RS_RS25270at 250.241 kb on - strand, within RS_RS25270

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galactose (C)
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247,369 - -1.4
247,916 - RS_RS01115 0.54 -1.4
247,982 - RS_RS01115 0.62 +2.4
247,987 + RS_RS01115 0.62 -0.8
247,987 + RS_RS01115 0.62 -1.1
247,987 + RS_RS01115 0.62 +0.1
247,988 - RS_RS01115 0.63 -0.1
247,988 - RS_RS01115 0.63 -1.9
247,988 - RS_RS01115 0.63 -0.8
247,988 - RS_RS01115 0.63 -1.7
247,988 - RS_RS01115 0.63 +0.1
247,988 - RS_RS01115 0.63 -0.2
247,988 - RS_RS01115 0.63 +0.1
248,149 + RS_RS01115 0.82 +2.4
248,149 + RS_RS01115 0.82 +0.8
248,149 + RS_RS01115 0.82 +0.4
248,150 - RS_RS01115 0.82 -0.4
248,266 + -0.3
248,267 - -0.6
248,267 - -0.4
248,337 + -0.7
248,337 + -0.2
248,337 + -0.0
248,360 + +1.9
248,361 - +0.8
248,361 - -0.5
248,561 + RS_RS01120 0.20 +1.1
248,627 - RS_RS01120 0.27 -2.2
248,842 + RS_RS01120 0.47 -1.7
249,653 + +0.1
249,654 - +0.2
249,654 - +1.4
249,656 - +1.4
249,874 - -0.2
249,874 - +0.6
249,953 + -0.8
249,978 + -0.1
250,032 + +0.8
250,032 + -0.5
250,032 + +2.4
250,033 - -3.0
250,033 - -0.1
250,114 + RS_RS25270 0.15 +0.5
250,115 - RS_RS25270 0.15 -0.5
250,119 + RS_RS25270 0.16 -0.2
250,166 - RS_RS25270 0.33 +0.1
250,166 - RS_RS25270 0.33 -0.3
250,240 + RS_RS25270 0.60 +0.1
250,240 + RS_RS25270 0.60 -1.6
250,240 + RS_RS25270 0.60 -0.1
250,241 - RS_RS25270 0.60 -0.4
250,241 - RS_RS25270 0.60 +1.4

Or see this region's nucleotide sequence