Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS00125

Experiment: D-Galactose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS00120 and RS_RS00125 are separated by 165 nucleotidesRS_RS00125 and RS_RS00130 are separated by 226 nucleotidesRS_RS00130 and RS_RS00135 overlap by 4 nucleotides RS_RS00120: RS_RS00120 - sel1 repeat family protein, at 28,773 to 29,531 _RS00120 RS_RS00125: RS_RS00125 - hypothetical protein, at 29,697 to 30,218 _RS00125 RS_RS00130: RS_RS00130 - methionine biosynthesis protein MetW, at 30,445 to 31,092 _RS00130 RS_RS00135: RS_RS00135 - homoserine O-acetyltransferase, at 31,089 to 32,300 _RS00135 Position (kb) 29 30 31Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 28.704 kb on + strandat 28.774 kb on + strandat 28.775 kb on - strandat 28.775 kb on - strandat 28.775 kb on - strandat 28.775 kb on - strandat 28.775 kb on - strandat 28.823 kb on - strandat 28.951 kb on + strand, within RS_RS00120at 28.951 kb on + strand, within RS_RS00120at 28.952 kb on - strand, within RS_RS00120at 28.966 kb on + strand, within RS_RS00120at 28.966 kb on + strand, within RS_RS00120at 28.966 kb on + strand, within RS_RS00120at 28.966 kb on + strand, within RS_RS00120at 28.966 kb on + strand, within RS_RS00120at 28.967 kb on - strand, within RS_RS00120at 28.967 kb on - strand, within RS_RS00120at 29.057 kb on - strand, within RS_RS00120at 29.057 kb on - strand, within RS_RS00120at 29.057 kb on - strand, within RS_RS00120at 29.057 kb on - strand, within RS_RS00120at 29.114 kb on - strand, within RS_RS00120at 29.114 kb on - strand, within RS_RS00120at 29.176 kb on + strand, within RS_RS00120at 29.284 kb on + strand, within RS_RS00120at 29.284 kb on + strand, within RS_RS00120at 29.284 kb on + strand, within RS_RS00120at 29.284 kb on + strand, within RS_RS00120at 29.285 kb on - strand, within RS_RS00120at 29.285 kb on - strand, within RS_RS00120at 29.744 kb on + strandat 29.811 kb on - strand, within RS_RS00125at 29.869 kb on + strand, within RS_RS00125at 29.870 kb on - strand, within RS_RS00125at 29.870 kb on - strand, within RS_RS00125at 29.870 kb on - strand, within RS_RS00125at 29.870 kb on - strand, within RS_RS00125at 29.966 kb on - strand, within RS_RS00125at 29.981 kb on - strand, within RS_RS00125at 30.137 kb on + strand, within RS_RS00125at 30.219 kb on + strandat 30.219 kb on + strandat 30.220 kb on - strandat 30.265 kb on + strandat 30.266 kb on - strandat 30.275 kb on + strandat 30.275 kb on + strandat 30.375 kb on - strandat 30.378 kb on + strandat 30.379 kb on - strandat 30.396 kb on - strandat 30.611 kb on + strand, within RS_RS00130at 30.620 kb on + strand, within RS_RS00130at 30.620 kb on + strand, within RS_RS00130at 30.620 kb on + strand, within RS_RS00130at 30.621 kb on - strand, within RS_RS00130at 30.681 kb on - strand, within RS_RS00130at 30.790 kb on - strand, within RS_RS00130at 30.903 kb on - strand, within RS_RS00130at 30.932 kb on - strand, within RS_RS00130at 31.120 kb on + strandat 31.121 kb on - strandat 31.124 kb on - strandat 31.201 kb on + strandat 31.202 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galactose (C)
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28,704 + -0.1
28,774 + -0.1
28,775 - -1.2
28,775 - +0.4
28,775 - -0.2
28,775 - -0.7
28,775 - +1.4
28,823 - +0.8
28,951 + RS_RS00120 0.23 +2.4
28,951 + RS_RS00120 0.23 -0.8
28,952 - RS_RS00120 0.24 -0.1
28,966 + RS_RS00120 0.25 +1.4
28,966 + RS_RS00120 0.25 +0.0
28,966 + RS_RS00120 0.25 -1.0
28,966 + RS_RS00120 0.25 +0.8
28,966 + RS_RS00120 0.25 -3.5
28,967 - RS_RS00120 0.26 -0.6
28,967 - RS_RS00120 0.26 -0.4
29,057 - RS_RS00120 0.37 -0.6
29,057 - RS_RS00120 0.37 -0.8
29,057 - RS_RS00120 0.37 +2.4
29,057 - RS_RS00120 0.37 -0.0
29,114 - RS_RS00120 0.45 -1.1
29,114 - RS_RS00120 0.45 -0.5
29,176 + RS_RS00120 0.53 +0.4
29,284 + RS_RS00120 0.67 -0.0
29,284 + RS_RS00120 0.67 -0.8
29,284 + RS_RS00120 0.67 -0.8
29,284 + RS_RS00120 0.67 -0.1
29,285 - RS_RS00120 0.67 -1.0
29,285 - RS_RS00120 0.67 +2.4
29,744 + +0.8
29,811 - RS_RS00125 0.22 -1.4
29,869 + RS_RS00125 0.33 -0.4
29,870 - RS_RS00125 0.33 -0.8
29,870 - RS_RS00125 0.33 -0.6
29,870 - RS_RS00125 0.33 -1.1
29,870 - RS_RS00125 0.33 +1.0
29,966 - RS_RS00125 0.52 +0.6
29,981 - RS_RS00125 0.54 -1.9
30,137 + RS_RS00125 0.84 -0.4
30,219 + +0.5
30,219 + -0.3
30,220 - -0.2
30,265 + -0.8
30,266 - +0.4
30,275 + -0.2
30,275 + +1.1
30,375 - +0.8
30,378 + -0.4
30,379 - -0.0
30,396 - +0.8
30,611 + RS_RS00130 0.26 -1.1
30,620 + RS_RS00130 0.27 +1.4
30,620 + RS_RS00130 0.27 -2.1
30,620 + RS_RS00130 0.27 -3.1
30,621 - RS_RS00130 0.27 -3.9
30,681 - RS_RS00130 0.36 -0.2
30,790 - RS_RS00130 0.53 -0.6
30,903 - RS_RS00130 0.71 +0.8
30,932 - RS_RS00130 0.75 -1.3
31,120 + +0.0
31,121 - -3.7
31,124 - -3.0
31,201 + +1.4
31,202 - -5.1

Or see this region's nucleotide sequence