Experiment: L-Aspartic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS21375 and RS_RS21380 are separated by 273 nucleotides RS_RS21380 and RS_RS21385 are separated by 90 nucleotides RS_RS21385 and RS_RS21390 overlap by 4 nucleotides
RS_RS21375: RS_RS21375 - type I glutamate--ammonia ligase, at 1,122,798 to 1,124,273
_RS21375
RS_RS21380: RS_RS21380 - LysR family transcriptional regulator, at 1,124,547 to 1,125,473
_RS21380
RS_RS21385: RS_RS21385 - oxidoreductase, at 1,125,564 to 1,126,430
_RS21385
RS_RS21390: RS_RS21390 - class II aldolase family protein, at 1,126,427 to 1,127,095
_RS21390
Position (kb)
1124
1125
1126 Strain fitness (log2 ratio)
-2
-1
0
1 at 1123.690 kb on + strand, within RS_RS21375 at 1123.690 kb on + strand, within RS_RS21375 at 1123.777 kb on + strand, within RS_RS21375 at 1123.778 kb on - strand, within RS_RS21375 at 1123.778 kb on - strand, within RS_RS21375 at 1123.781 kb on - strand, within RS_RS21375 at 1123.849 kb on + strand, within RS_RS21375 at 1123.850 kb on - strand, within RS_RS21375 at 1124.243 kb on - strand at 1124.243 kb on - strand at 1124.300 kb on + strand at 1124.300 kb on + strand at 1124.300 kb on + strand at 1124.301 kb on - strand at 1124.301 kb on - strand at 1124.310 kb on + strand at 1124.311 kb on + strand at 1124.311 kb on + strand at 1124.312 kb on - strand at 1124.312 kb on - strand at 1124.844 kb on - strand, within RS_RS21380 at 1124.935 kb on + strand, within RS_RS21380 at 1124.936 kb on - strand, within RS_RS21380 at 1125.124 kb on + strand, within RS_RS21380 at 1125.589 kb on - strand at 1125.589 kb on - strand at 1125.631 kb on - strand at 1125.684 kb on + strand, within RS_RS21385 at 1125.684 kb on + strand, within RS_RS21385 at 1126.294 kb on - strand, within RS_RS21385 at 1126.344 kb on + strand at 1126.344 kb on + strand at 1126.380 kb on + strand at 1126.427 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (C) remove 1,123,690 + RS_RS21375 0.60 +0.3 1,123,690 + RS_RS21375 0.60 +0.3 1,123,777 + RS_RS21375 0.66 -0.0 1,123,778 - RS_RS21375 0.66 -0.3 1,123,778 - RS_RS21375 0.66 +0.4 1,123,781 - RS_RS21375 0.67 -0.3 1,123,849 + RS_RS21375 0.71 +0.0 1,123,850 - RS_RS21375 0.71 -0.7 1,124,243 - -0.0 1,124,243 - -0.9 1,124,300 + -1.8 1,124,300 + -0.9 1,124,300 + -0.7 1,124,301 - +0.2 1,124,301 - +0.3 1,124,310 + +0.6 1,124,311 + -0.8 1,124,311 + +1.3 1,124,312 - +1.3 1,124,312 - -1.5 1,124,844 - RS_RS21380 0.32 -0.1 1,124,935 + RS_RS21380 0.42 -1.7 1,124,936 - RS_RS21380 0.42 +0.3 1,125,124 + RS_RS21380 0.62 -1.9 1,125,589 - -0.0 1,125,589 - +1.3 1,125,631 - -1.7 1,125,684 + RS_RS21385 0.14 -0.8 1,125,684 + RS_RS21385 0.14 -0.3 1,126,294 - RS_RS21385 0.84 -0.9 1,126,344 + -0.2 1,126,344 + -2.0 1,126,380 + -0.1 1,126,427 - +0.3
Or see this region's nucleotide sequence