Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS14670 and RS_RS14675 are separated by 2 nucleotides RS_RS14675 and RS_RS14680 are separated by 245 nucleotides RS_RS14680 and RS_RS14685 are separated by 13 nucleotides
RS_RS14670: RS_RS14670 - cytochrome b, at 3,158,487 to 3,159,890
_RS14670
RS_RS14675: RS_RS14675 - ubiquinol-cytochrome c reductase iron-sulfur subunit, at 3,159,893 to 3,160,501
_RS14675
RS_RS14680: RS_RS14680 - large conductance mechanosensitive channel protein MscL, at 3,160,747 to 3,161,172
_RS14680
RS_RS14685: RS_RS14685 - Nif3-like dinuclear metal center hexameric protein, at 3,161,186 to 3,161,932
_RS14685
Position (kb)
3159
3160
3161 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3158.971 kb on + strand, within RS_RS14670 at 3159.043 kb on + strand, within RS_RS14670 at 3159.128 kb on - strand, within RS_RS14670 at 3159.128 kb on - strand, within RS_RS14670 at 3159.409 kb on + strand, within RS_RS14670 at 3159.410 kb on - strand, within RS_RS14670 at 3159.436 kb on + strand, within RS_RS14670 at 3159.551 kb on - strand, within RS_RS14670 at 3159.593 kb on - strand, within RS_RS14670 at 3159.593 kb on - strand, within RS_RS14670 at 3159.593 kb on - strand, within RS_RS14670 at 3159.692 kb on - strand, within RS_RS14670 at 3159.790 kb on + strand at 3159.951 kb on + strand at 3159.952 kb on - strand at 3160.270 kb on + strand, within RS_RS14675 at 3160.271 kb on - strand, within RS_RS14675 at 3160.422 kb on - strand, within RS_RS14675 at 3160.529 kb on + strand at 3160.530 kb on - strand at 3160.563 kb on + strand at 3160.606 kb on + strand at 3160.607 kb on - strand at 3160.607 kb on - strand at 3160.680 kb on - strand at 3160.754 kb on + strand at 3160.754 kb on + strand at 3160.754 kb on + strand at 3160.755 kb on - strand at 3161.300 kb on - strand, within RS_RS14685 at 3161.361 kb on + strand, within RS_RS14685
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 3,158,971 + RS_RS14670 0.34 +0.3 3,159,043 + RS_RS14670 0.40 -1.1 3,159,128 - RS_RS14670 0.46 -1.5 3,159,128 - RS_RS14670 0.46 -0.5 3,159,409 + RS_RS14670 0.66 -0.5 3,159,410 - RS_RS14670 0.66 +1.9 3,159,436 + RS_RS14670 0.68 +0.9 3,159,551 - RS_RS14670 0.76 -0.8 3,159,593 - RS_RS14670 0.79 -2.8 3,159,593 - RS_RS14670 0.79 -2.4 3,159,593 - RS_RS14670 0.79 -1.0 3,159,692 - RS_RS14670 0.86 -1.4 3,159,790 + +0.9 3,159,951 + +0.9 3,159,952 - -2.8 3,160,270 + RS_RS14675 0.62 -1.1 3,160,271 - RS_RS14675 0.62 -0.3 3,160,422 - RS_RS14675 0.87 -1.2 3,160,529 + -1.7 3,160,530 - +0.3 3,160,563 + -1.3 3,160,606 + -0.5 3,160,607 - +0.0 3,160,607 - +1.9 3,160,680 - +0.2 3,160,754 + -0.4 3,160,754 + -0.5 3,160,754 + -0.2 3,160,755 - +0.2 3,161,300 - RS_RS14685 0.15 -0.5 3,161,361 + RS_RS14685 0.23 -0.1
Or see this region's nucleotide sequence