Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS09290

Experiment: L-Alanine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS09285 and RS_RS09290 are separated by 205 nucleotidesRS_RS09290 and RS_RS25495 are separated by 288 nucleotidesRS_RS25495 and RS_RS09295 are separated by 250 nucleotides RS_RS09285: RS_RS09285 - pyridoxal phosphate-dependent aminotransferase, at 2,019,456 to 2,020,334 _RS09285 RS_RS09290: RS_RS09290 - alpha/beta hydrolase, at 2,020,540 to 2,021,427 _RS09290 RS_RS25495: RS_RS25495 - hypothetical protein, at 2,021,716 to 2,022,018 _RS25495 RS_RS09295: RS_RS09295 - hypothetical protein, at 2,022,269 to 2,022,985 _RS09295 Position (kb) 2020 2021 2022Strain fitness (log2 ratio) -3 -2 -1 0 1at 2019.976 kb on + strand, within RS_RS09285at 2019.976 kb on + strand, within RS_RS09285at 2020.137 kb on + strand, within RS_RS09285at 2020.138 kb on - strand, within RS_RS09285at 2020.332 kb on + strandat 2020.333 kb on - strandat 2020.333 kb on - strandat 2020.382 kb on + strandat 2020.382 kb on + strandat 2020.394 kb on + strandat 2020.394 kb on + strandat 2020.395 kb on - strandat 2020.395 kb on - strandat 2020.720 kb on + strand, within RS_RS09290at 2020.721 kb on - strand, within RS_RS09290at 2020.743 kb on - strand, within RS_RS09290at 2020.773 kb on + strand, within RS_RS09290at 2020.774 kb on - strand, within RS_RS09290at 2020.774 kb on - strand, within RS_RS09290at 2020.789 kb on + strand, within RS_RS09290at 2020.810 kb on + strand, within RS_RS09290at 2020.811 kb on - strand, within RS_RS09290at 2020.844 kb on + strand, within RS_RS09290at 2020.845 kb on - strand, within RS_RS09290at 2021.063 kb on + strand, within RS_RS09290at 2021.541 kb on + strandat 2021.749 kb on + strand, within RS_RS25495at 2021.750 kb on - strand, within RS_RS25495at 2021.772 kb on + strand, within RS_RS25495at 2021.782 kb on - strand, within RS_RS25495at 2021.856 kb on + strand, within RS_RS25495at 2021.905 kb on - strand, within RS_RS25495at 2021.905 kb on - strand, within RS_RS25495at 2021.958 kb on + strand, within RS_RS25495at 2021.997 kb on + strandat 2022.004 kb on - strandat 2022.037 kb on + strandat 2022.037 kb on + strandat 2022.038 kb on - strandat 2022.071 kb on + strandat 2022.072 kb on - strandat 2022.115 kb on - strandat 2022.145 kb on - strandat 2022.211 kb on + strandat 2022.211 kb on + strandat 2022.228 kb on + strandat 2022.228 kb on + strandat 2022.228 kb on + strandat 2022.229 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (C)
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2,019,976 + RS_RS09285 0.59 +1.3
2,019,976 + RS_RS09285 0.59 +0.1
2,020,137 + RS_RS09285 0.77 -0.2
2,020,138 - RS_RS09285 0.78 -0.0
2,020,332 + -1.7
2,020,333 - -1.0
2,020,333 - -0.2
2,020,382 + +0.3
2,020,382 + -1.1
2,020,394 + -1.0
2,020,394 + -0.2
2,020,395 - -0.1
2,020,395 - +1.3
2,020,720 + RS_RS09290 0.20 -0.3
2,020,721 - RS_RS09290 0.20 +0.2
2,020,743 - RS_RS09290 0.23 +0.3
2,020,773 + RS_RS09290 0.26 +0.0
2,020,774 - RS_RS09290 0.26 -1.0
2,020,774 - RS_RS09290 0.26 -0.8
2,020,789 + RS_RS09290 0.28 +0.3
2,020,810 + RS_RS09290 0.30 +0.1
2,020,811 - RS_RS09290 0.31 +0.1
2,020,844 + RS_RS09290 0.34 +1.3
2,020,845 - RS_RS09290 0.34 +0.3
2,021,063 + RS_RS09290 0.59 +0.6
2,021,541 + -0.3
2,021,749 + RS_RS25495 0.11 -0.1
2,021,750 - RS_RS25495 0.11 -0.5
2,021,772 + RS_RS25495 0.18 -0.3
2,021,782 - RS_RS25495 0.22 -1.0
2,021,856 + RS_RS25495 0.46 -2.9
2,021,905 - RS_RS25495 0.62 -0.3
2,021,905 - RS_RS25495 0.62 -0.0
2,021,958 + RS_RS25495 0.80 -2.5
2,021,997 + -0.0
2,022,004 - -0.3
2,022,037 + -0.2
2,022,037 + -0.2
2,022,038 - +0.3
2,022,071 + +0.3
2,022,072 - -1.3
2,022,115 - -0.7
2,022,145 - -1.0
2,022,211 + +0.4
2,022,211 + -0.4
2,022,228 + -1.3
2,022,228 + -3.1
2,022,228 + +0.4
2,022,229 - -0.4

Or see this region's nucleotide sequence