Experiment: succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RS_RS21395 and RS_RS21400 overlap by 4 nucleotides RS_RS21400 and RS_RS21405 overlap by 4 nucleotides RS_RS21405 and RS_RS21410 overlap by 10 nucleotides RS_RS21410 and RS_RS21415 overlap by 17 nucleotides
RS_RS21395: RS_RS21395 - 2-hydroxymuconic semialdehyde dehydrogenase, at 1,127,111 to 1,128,577
_RS21395
RS_RS21400: RS_RS21400 - 2-keto-4-pentenoate hydratase, at 1,128,574 to 1,129,371
_RS21400
RS_RS21405: RS_RS21405 - 4-oxalocrotonate decarboxylase, at 1,129,368 to 1,130,159
_RS21405
RS_RS21410: RS_RS21410 - tautomerase, at 1,130,150 to 1,130,362
_RS21410
RS_RS21415: RS_RS21415 - acetaldehyde dehydrogenase, at 1,130,346 to 1,131,266
_RS21415
Position (kb)
1129
1130
1131 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1128.371 kb on + strand, within RS_RS21395 at 1128.371 kb on + strand, within RS_RS21395 at 1128.373 kb on + strand, within RS_RS21395 at 1128.374 kb on - strand, within RS_RS21395 at 1128.530 kb on + strand at 1128.662 kb on - strand, within RS_RS21400 at 1129.476 kb on + strand, within RS_RS21405 at 1129.477 kb on - strand, within RS_RS21405 at 1129.788 kb on + strand, within RS_RS21405 at 1129.789 kb on - strand, within RS_RS21405 at 1129.789 kb on - strand, within RS_RS21405 at 1130.361 kb on - strand at 1130.452 kb on + strand, within RS_RS21415 at 1130.453 kb on - strand, within RS_RS21415 at 1130.670 kb on + strand, within RS_RS21415 at 1130.670 kb on + strand, within RS_RS21415 at 1130.794 kb on - strand, within RS_RS21415 at 1130.794 kb on - strand, within RS_RS21415 at 1131.048 kb on + strand, within RS_RS21415 at 1131.048 kb on + strand, within RS_RS21415 at 1131.048 kb on + strand, within RS_RS21415 at 1131.049 kb on - strand, within RS_RS21415
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C) remove 1,128,371 + RS_RS21395 0.86 +0.2 1,128,371 + RS_RS21395 0.86 +0.7 1,128,373 + RS_RS21395 0.86 +0.4 1,128,374 - RS_RS21395 0.86 +0.4 1,128,530 + -0.5 1,128,662 - RS_RS21400 0.11 -0.2 1,129,476 + RS_RS21405 0.14 +0.1 1,129,477 - RS_RS21405 0.14 -0.5 1,129,788 + RS_RS21405 0.53 -0.3 1,129,789 - RS_RS21405 0.53 +0.4 1,129,789 - RS_RS21405 0.53 -0.3 1,130,361 - +0.3 1,130,452 + RS_RS21415 0.12 -0.5 1,130,453 - RS_RS21415 0.12 -1.2 1,130,670 + RS_RS21415 0.35 -2.5 1,130,670 + RS_RS21415 0.35 -0.3 1,130,794 - RS_RS21415 0.49 +1.8 1,130,794 - RS_RS21415 0.49 -0.8 1,131,048 + RS_RS21415 0.76 -1.0 1,131,048 + RS_RS21415 0.76 +0.5 1,131,048 + RS_RS21415 0.76 -0.4 1,131,049 - RS_RS21415 0.76 +1.0
Or see this region's nucleotide sequence