Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS18920

Experiment: succinate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS18915 and RS_RS18920 overlap by 4 nucleotidesRS_RS18920 and RS_RS18925 overlap by 4 nucleotidesRS_RS18925 and RS_RS18930 are separated by 4 nucleotidesRS_RS18930 and RS_RS18935 are separated by 38 nucleotides RS_RS18915: RS_RS18915 - acyltransferase, at 472,295 to 474,004 _RS18915 RS_RS18920: RS_RS18920 - AMP-dependent synthetase, at 474,001 to 475,692 _RS18920 RS_RS18925: RS_RS18925 - membrane protein, at 475,689 to 476,306 _RS18925 RS_RS18930: RS_RS18930 - acyl carrier protein, at 476,311 to 476,556 _RS18930 RS_RS18935: RS_RS18935 - acyl carrier protein, at 476,595 to 476,852 _RS18935 Position (kb) 474 475 476Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 473.067 kb on + strand, within RS_RS18915at 473.068 kb on - strand, within RS_RS18915at 473.068 kb on - strand, within RS_RS18915at 473.113 kb on - strand, within RS_RS18915at 473.361 kb on - strand, within RS_RS18915at 473.361 kb on - strand, within RS_RS18915at 473.361 kb on - strand, within RS_RS18915at 473.392 kb on - strand, within RS_RS18915at 473.392 kb on - strand, within RS_RS18915at 473.523 kb on + strand, within RS_RS18915at 473.607 kb on + strand, within RS_RS18915at 473.608 kb on - strand, within RS_RS18915at 473.637 kb on + strand, within RS_RS18915at 473.638 kb on - strand, within RS_RS18915at 473.968 kb on + strandat 473.970 kb on + strandat 473.970 kb on + strandat 473.970 kb on + strandat 473.970 kb on + strandat 473.971 kb on - strandat 473.971 kb on - strandat 473.971 kb on - strandat 473.971 kb on - strandat 474.071 kb on - strandat 474.297 kb on - strand, within RS_RS18920at 474.297 kb on - strand, within RS_RS18920at 474.459 kb on - strand, within RS_RS18920at 474.608 kb on + strand, within RS_RS18920at 474.608 kb on + strand, within RS_RS18920at 474.609 kb on - strand, within RS_RS18920at 474.887 kb on + strand, within RS_RS18920at 474.888 kb on - strand, within RS_RS18920at 474.888 kb on - strand, within RS_RS18920at 475.100 kb on + strand, within RS_RS18920at 475.100 kb on + strand, within RS_RS18920at 475.101 kb on - strand, within RS_RS18920at 475.101 kb on - strand, within RS_RS18920at 475.101 kb on - strand, within RS_RS18920at 475.163 kb on + strand, within RS_RS18920at 475.163 kb on + strand, within RS_RS18920at 475.164 kb on - strand, within RS_RS18920at 475.167 kb on + strand, within RS_RS18920at 475.167 kb on + strand, within RS_RS18920at 475.167 kb on + strand, within RS_RS18920at 475.167 kb on + strand, within RS_RS18920at 475.167 kb on + strand, within RS_RS18920at 475.167 kb on + strand, within RS_RS18920at 475.167 kb on + strand, within RS_RS18920at 475.168 kb on - strand, within RS_RS18920at 475.168 kb on - strand, within RS_RS18920at 475.168 kb on - strand, within RS_RS18920at 475.168 kb on - strand, within RS_RS18920at 475.168 kb on - strand, within RS_RS18920at 475.168 kb on - strand, within RS_RS18920at 475.270 kb on - strand, within RS_RS18920at 475.270 kb on - strand, within RS_RS18920at 475.307 kb on + strand, within RS_RS18920at 475.418 kb on + strand, within RS_RS18920at 475.545 kb on - strandat 475.588 kb on + strandat 475.589 kb on - strandat 475.673 kb on - strandat 475.673 kb on - strandat 475.778 kb on - strand, within RS_RS18925at 475.795 kb on + strand, within RS_RS18925at 475.796 kb on - strand, within RS_RS18925at 475.796 kb on - strand, within RS_RS18925at 475.796 kb on - strand, within RS_RS18925at 475.876 kb on - strand, within RS_RS18925at 476.163 kb on + strand, within RS_RS18925at 476.191 kb on + strand, within RS_RS18925at 476.192 kb on - strand, within RS_RS18925at 476.627 kb on + strand, within RS_RS18935at 476.627 kb on + strand, within RS_RS18935at 476.628 kb on - strand, within RS_RS18935at 476.628 kb on - strand, within RS_RS18935at 476.645 kb on - strand, within RS_RS18935

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C)
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473,067 + RS_RS18915 0.45 +0.8
473,068 - RS_RS18915 0.45 +1.0
473,068 - RS_RS18915 0.45 -2.4
473,113 - RS_RS18915 0.48 +0.9
473,361 - RS_RS18915 0.62 +0.0
473,361 - RS_RS18915 0.62 -0.5
473,361 - RS_RS18915 0.62 -0.5
473,392 - RS_RS18915 0.64 -0.5
473,392 - RS_RS18915 0.64 +2.8
473,523 + RS_RS18915 0.72 +1.1
473,607 + RS_RS18915 0.77 -0.5
473,608 - RS_RS18915 0.77 +0.3
473,637 + RS_RS18915 0.78 +0.4
473,638 - RS_RS18915 0.79 +0.4
473,968 + -0.5
473,970 + -1.6
473,970 + +1.5
473,970 + +1.1
473,970 + -0.6
473,971 - +2.1
473,971 - +2.1
473,971 - +0.1
473,971 - +0.1
474,071 - +0.8
474,297 - RS_RS18920 0.17 +2.1
474,297 - RS_RS18920 0.17 -0.5
474,459 - RS_RS18920 0.27 +1.1
474,608 + RS_RS18920 0.36 -0.1
474,608 + RS_RS18920 0.36 +0.1
474,609 - RS_RS18920 0.36 +0.4
474,887 + RS_RS18920 0.52 -1.6
474,888 - RS_RS18920 0.52 -0.4
474,888 - RS_RS18920 0.52 +1.1
475,100 + RS_RS18920 0.65 -0.4
475,100 + RS_RS18920 0.65 +0.5
475,101 - RS_RS18920 0.65 +2.1
475,101 - RS_RS18920 0.65 +1.1
475,101 - RS_RS18920 0.65 -1.2
475,163 + RS_RS18920 0.69 -0.1
475,163 + RS_RS18920 0.69 -0.2
475,164 - RS_RS18920 0.69 +2.4
475,167 + RS_RS18920 0.69 +2.1
475,167 + RS_RS18920 0.69 +2.1
475,167 + RS_RS18920 0.69 +0.5
475,167 + RS_RS18920 0.69 -0.7
475,167 + RS_RS18920 0.69 +1.4
475,167 + RS_RS18920 0.69 +2.1
475,167 + RS_RS18920 0.69 -0.8
475,168 - RS_RS18920 0.69 +0.1
475,168 - RS_RS18920 0.69 -0.1
475,168 - RS_RS18920 0.69 -0.2
475,168 - RS_RS18920 0.69 +1.1
475,168 - RS_RS18920 0.69 -0.1
475,168 - RS_RS18920 0.69 +0.6
475,270 - RS_RS18920 0.75 -2.8
475,270 - RS_RS18920 0.75 +1.1
475,307 + RS_RS18920 0.77 -0.4
475,418 + RS_RS18920 0.84 +0.5
475,545 - +1.1
475,588 + +1.0
475,589 - +0.7
475,673 - -0.1
475,673 - +0.7
475,778 - RS_RS18925 0.14 +0.3
475,795 + RS_RS18925 0.17 +1.1
475,796 - RS_RS18925 0.17 -0.2
475,796 - RS_RS18925 0.17 -1.8
475,796 - RS_RS18925 0.17 -0.7
475,876 - RS_RS18925 0.30 -0.9
476,163 + RS_RS18925 0.77 +0.3
476,191 + RS_RS18925 0.81 +1.1
476,192 - RS_RS18925 0.81 +0.3
476,627 + RS_RS18935 0.12 +2.1
476,627 + RS_RS18935 0.12 +0.6
476,628 - RS_RS18935 0.13 -0.7
476,628 - RS_RS18935 0.13 -1.3
476,645 - RS_RS18935 0.19 +0.8

Or see this region's nucleotide sequence