Experiment: MBOA 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3543 and Psyr_3544 are separated by 16 nucleotides Psyr_3544 and Psyr_3545 are separated by 101 nucleotides Psyr_3545 and Psyr_3546 are separated by 138 nucleotides
Psyr_3543: Psyr_3543 - transcriptional regulator, AraC family, at 4,220,788 to 4,221,675
_3543
Psyr_3544: Psyr_3544 - putative integral membrane protein, at 4,221,692 to 4,222,666
_3544
Psyr_3545: Psyr_3545 - Short-chain dehydrogenase/reductase SDR, at 4,222,768 to 4,223,511
_3545
Psyr_3546: Psyr_3546 - transcriptional regulator, AraC family, at 4,223,650 to 4,224,558
_3546
Position (kb)
4221
4222
4223 Strain fitness (log2 ratio)
-2
-1
0
1 at 4220.787 kb on + strand at 4220.905 kb on + strand, within Psyr_3543 at 4220.974 kb on + strand, within Psyr_3543 at 4220.974 kb on + strand, within Psyr_3543 at 4220.974 kb on + strand, within Psyr_3543 at 4220.975 kb on - strand, within Psyr_3543 at 4220.975 kb on - strand, within Psyr_3543 at 4220.989 kb on + strand, within Psyr_3543 at 4220.990 kb on - strand, within Psyr_3543 at 4221.089 kb on + strand, within Psyr_3543 at 4221.090 kb on - strand, within Psyr_3543 at 4221.370 kb on + strand, within Psyr_3543 at 4221.370 kb on + strand, within Psyr_3543 at 4221.371 kb on - strand, within Psyr_3543 at 4221.371 kb on - strand, within Psyr_3543 at 4221.371 kb on - strand, within Psyr_3543 at 4221.371 kb on - strand, within Psyr_3543 at 4221.434 kb on - strand, within Psyr_3543 at 4221.434 kb on - strand, within Psyr_3543 at 4221.550 kb on + strand, within Psyr_3543 at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.693 kb on + strand at 4221.694 kb on - strand at 4221.694 kb on - strand at 4221.694 kb on - strand at 4221.694 kb on - strand at 4221.827 kb on - strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.828 kb on + strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.829 kb on - strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.832 kb on + strand, within Psyr_3544 at 4221.833 kb on - strand, within Psyr_3544 at 4221.835 kb on - strand, within Psyr_3544 at 4221.989 kb on - strand, within Psyr_3544 at 4222.007 kb on - strand, within Psyr_3544 at 4222.393 kb on - strand, within Psyr_3544 at 4222.412 kb on - strand, within Psyr_3544 at 4222.412 kb on - strand, within Psyr_3544 at 4222.647 kb on + strand at 4222.706 kb on + strand at 4222.738 kb on + strand at 4222.739 kb on - strand at 4222.746 kb on + strand at 4222.746 kb on + strand at 4222.747 kb on - strand at 4222.747 kb on - strand at 4222.978 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.007 kb on + strand, within Psyr_3545 at 4223.008 kb on - strand, within Psyr_3545 at 4223.009 kb on + strand, within Psyr_3545 at 4223.010 kb on - strand, within Psyr_3545 at 4223.049 kb on - strand, within Psyr_3545 at 4223.417 kb on + strand, within Psyr_3545 at 4223.422 kb on - strand, within Psyr_3545 at 4223.422 kb on - strand, within Psyr_3545 at 4223.497 kb on + strand at 4223.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MBOA 2.5 mM remove 4,220,787 + +0.2 4,220,905 + Psyr_3543 0.13 -0.1 4,220,974 + Psyr_3543 0.21 +1.0 4,220,974 + Psyr_3543 0.21 -0.4 4,220,974 + Psyr_3543 0.21 +0.6 4,220,975 - Psyr_3543 0.21 +0.4 4,220,975 - Psyr_3543 0.21 -0.5 4,220,989 + Psyr_3543 0.23 -0.5 4,220,990 - Psyr_3543 0.23 +0.9 4,221,089 + Psyr_3543 0.34 +0.2 4,221,090 - Psyr_3543 0.34 +0.7 4,221,370 + Psyr_3543 0.66 -0.4 4,221,370 + Psyr_3543 0.66 +1.0 4,221,371 - Psyr_3543 0.66 -1.5 4,221,371 - Psyr_3543 0.66 +0.9 4,221,371 - Psyr_3543 0.66 -0.4 4,221,371 - Psyr_3543 0.66 +0.7 4,221,434 - Psyr_3543 0.73 +0.3 4,221,434 - Psyr_3543 0.73 +1.5 4,221,550 + Psyr_3543 0.86 -0.7 4,221,693 + +0.3 4,221,693 + -0.6 4,221,693 + +0.5 4,221,693 + +0.3 4,221,693 + -0.3 4,221,693 + -0.5 4,221,694 - -0.7 4,221,694 - +0.3 4,221,694 - -0.7 4,221,694 - -0.4 4,221,827 - Psyr_3544 0.14 +0.7 4,221,828 + Psyr_3544 0.14 -1.2 4,221,828 + Psyr_3544 0.14 +0.8 4,221,828 + Psyr_3544 0.14 +0.9 4,221,828 + Psyr_3544 0.14 -1.8 4,221,828 + Psyr_3544 0.14 -0.4 4,221,828 + Psyr_3544 0.14 -0.5 4,221,828 + Psyr_3544 0.14 -0.2 4,221,828 + Psyr_3544 0.14 -0.7 4,221,828 + Psyr_3544 0.14 +0.6 4,221,828 + Psyr_3544 0.14 +0.4 4,221,829 - Psyr_3544 0.14 -0.1 4,221,829 - Psyr_3544 0.14 +0.7 4,221,829 - Psyr_3544 0.14 +0.3 4,221,829 - Psyr_3544 0.14 +0.9 4,221,829 - Psyr_3544 0.14 -0.4 4,221,829 - Psyr_3544 0.14 -0.1 4,221,829 - Psyr_3544 0.14 +0.7 4,221,832 + Psyr_3544 0.14 -0.2 4,221,832 + Psyr_3544 0.14 -0.2 4,221,832 + Psyr_3544 0.14 +0.1 4,221,833 - Psyr_3544 0.14 +0.6 4,221,835 - Psyr_3544 0.15 -0.2 4,221,989 - Psyr_3544 0.30 +1.3 4,222,007 - Psyr_3544 0.32 +0.3 4,222,393 - Psyr_3544 0.72 +1.0 4,222,412 - Psyr_3544 0.74 -0.1 4,222,412 - Psyr_3544 0.74 -2.3 4,222,647 + -0.8 4,222,706 + +0.0 4,222,738 + -2.4 4,222,739 - +0.0 4,222,746 + +1.5 4,222,746 + -0.2 4,222,747 - -1.9 4,222,747 - +0.2 4,222,978 + Psyr_3545 0.28 -0.1 4,223,007 + Psyr_3545 0.32 -0.8 4,223,007 + Psyr_3545 0.32 -0.9 4,223,007 + Psyr_3545 0.32 -0.8 4,223,008 - Psyr_3545 0.32 -0.2 4,223,009 + Psyr_3545 0.32 +0.1 4,223,010 - Psyr_3545 0.33 -0.0 4,223,049 - Psyr_3545 0.38 -1.5 4,223,417 + Psyr_3545 0.87 -0.4 4,223,422 - Psyr_3545 0.88 -1.3 4,223,422 - Psyr_3545 0.88 -0.3 4,223,497 + -0.1 4,223,498 - -0.5
Or see this region's nucleotide sequence