Experiment: MBOA 1.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0499 and Psyr_0500 overlap by 4 nucleotides Psyr_0500 and Psyr_0501 are separated by 58 nucleotides
Psyr_0499: Psyr_0499 - Urea amidolyase-related protein, at 540,894 to 542,498
_0499
Psyr_0500: Psyr_0500 - biotin carboxyl carrier protein / biotin carboxylase, at 542,495 to 544,228
_0500
Psyr_0501: Psyr_0501 - MscS Mechanosensitive ion channel, at 544,287 to 547,691
_0501
Position (kb)
542
543
544
545 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 541.569 kb on + strand, within Psyr_0499 at 541.569 kb on + strand, within Psyr_0499 at 541.570 kb on - strand, within Psyr_0499 at 541.848 kb on + strand, within Psyr_0499 at 541.849 kb on - strand, within Psyr_0499 at 541.865 kb on - strand, within Psyr_0499 at 541.917 kb on + strand, within Psyr_0499 at 541.917 kb on + strand, within Psyr_0499 at 541.918 kb on - strand, within Psyr_0499 at 541.918 kb on - strand, within Psyr_0499 at 541.918 kb on - strand, within Psyr_0499 at 542.385 kb on + strand at 542.385 kb on + strand at 542.385 kb on + strand at 542.385 kb on + strand at 542.386 kb on - strand at 542.539 kb on + strand at 542.669 kb on + strand, within Psyr_0500 at 542.669 kb on + strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.670 kb on - strand, within Psyr_0500 at 542.854 kb on + strand, within Psyr_0500 at 542.855 kb on - strand, within Psyr_0500 at 542.855 kb on - strand, within Psyr_0500 at 542.855 kb on - strand, within Psyr_0500 at 543.227 kb on + strand, within Psyr_0500 at 543.227 kb on + strand, within Psyr_0500 at 543.228 kb on - strand, within Psyr_0500 at 543.293 kb on + strand, within Psyr_0500 at 543.294 kb on - strand, within Psyr_0500 at 543.294 kb on - strand, within Psyr_0500 at 543.550 kb on + strand, within Psyr_0500 at 543.551 kb on - strand, within Psyr_0500 at 543.551 kb on - strand, within Psyr_0500 at 543.558 kb on + strand, within Psyr_0500 at 543.793 kb on + strand, within Psyr_0500 at 543.794 kb on - strand, within Psyr_0500 at 544.188 kb on - strand at 544.343 kb on + strand at 544.420 kb on + strand at 544.447 kb on + strand at 544.447 kb on + strand at 544.447 kb on + strand at 544.447 kb on + strand at 544.448 kb on - strand at 544.448 kb on - strand at 544.448 kb on - strand at 544.554 kb on + strand at 544.555 kb on - strand at 544.555 kb on - strand at 544.555 kb on - strand at 544.555 kb on - strand at 544.555 kb on - strand at 544.584 kb on - strand at 544.606 kb on + strand at 544.659 kb on + strand, within Psyr_0501 at 544.659 kb on + strand, within Psyr_0501 at 544.660 kb on - strand, within Psyr_0501 at 545.057 kb on - strand, within Psyr_0501 at 545.057 kb on - strand, within Psyr_0501 at 545.080 kb on + strand, within Psyr_0501 at 545.121 kb on + strand, within Psyr_0501 at 545.122 kb on - strand, within Psyr_0501
Per-strain Table
Position Strand Gene LocusTag Fraction MBOA 1.2 mM remove 541,569 + Psyr_0499 0.42 +0.8 541,569 + Psyr_0499 0.42 +0.2 541,570 - Psyr_0499 0.42 -0.1 541,848 + Psyr_0499 0.59 +0.9 541,849 - Psyr_0499 0.60 -0.5 541,865 - Psyr_0499 0.60 -0.4 541,917 + Psyr_0499 0.64 -0.0 541,917 + Psyr_0499 0.64 -1.4 541,918 - Psyr_0499 0.64 +0.6 541,918 - Psyr_0499 0.64 +1.1 541,918 - Psyr_0499 0.64 -0.5 542,385 + -0.4 542,385 + -0.3 542,385 + -0.6 542,385 + +0.3 542,386 - -0.6 542,539 + +0.5 542,669 + Psyr_0500 0.10 +0.2 542,669 + Psyr_0500 0.10 -0.7 542,670 - Psyr_0500 0.10 -0.3 542,670 - Psyr_0500 0.10 +0.6 542,670 - Psyr_0500 0.10 +0.8 542,670 - Psyr_0500 0.10 +1.1 542,670 - Psyr_0500 0.10 +1.3 542,670 - Psyr_0500 0.10 -1.1 542,854 + Psyr_0500 0.21 -0.2 542,855 - Psyr_0500 0.21 +1.1 542,855 - Psyr_0500 0.21 -0.3 542,855 - Psyr_0500 0.21 -0.1 543,227 + Psyr_0500 0.42 -0.7 543,227 + Psyr_0500 0.42 +0.2 543,228 - Psyr_0500 0.42 +0.6 543,293 + Psyr_0500 0.46 -0.3 543,294 - Psyr_0500 0.46 -0.7 543,294 - Psyr_0500 0.46 +0.4 543,550 + Psyr_0500 0.61 +0.3 543,551 - Psyr_0500 0.61 -0.9 543,551 - Psyr_0500 0.61 -0.7 543,558 + Psyr_0500 0.61 +0.8 543,793 + Psyr_0500 0.75 -0.2 543,794 - Psyr_0500 0.75 -0.2 544,188 - -0.5 544,343 + +0.0 544,420 + +0.4 544,447 + -2.5 544,447 + +0.4 544,447 + -0.8 544,447 + -1.0 544,448 - +0.6 544,448 - +0.3 544,448 - -1.2 544,554 + -1.1 544,555 - -0.6 544,555 - -0.9 544,555 - +0.3 544,555 - +0.6 544,555 - -1.1 544,584 - -1.6 544,606 + -0.2 544,659 + Psyr_0501 0.11 +0.5 544,659 + Psyr_0501 0.11 +0.3 544,660 - Psyr_0501 0.11 +0.6 545,057 - Psyr_0501 0.23 +1.9 545,057 - Psyr_0501 0.23 +0.4 545,080 + Psyr_0501 0.23 -2.7 545,121 + Psyr_0501 0.24 +0.0 545,122 - Psyr_0501 0.25 +0.4
Or see this region's nucleotide sequence