Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0518

Experiment: no stress control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0517 and Psyr_0518 are separated by 142 nucleotidesPsyr_0518 and Psyr_0519 are separated by 384 nucleotides Psyr_0517: Psyr_0517 - Dihydrolipoamide acetyltransferase, at 574,465 to 576,138 _0517 Psyr_0518: Psyr_0518 - 2-oxo-acid dehydrogenase E1 component homodimeric type, at 576,281 to 578,926 _0518 Psyr_0519: Psyr_0519 - Glutamate-ammonia-ligase adenylyltransferase, at 579,311 to 582,268 _0519 Position (kb) 576 577 578 579Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 576.190 kb on - strandat 576.190 kb on - strandat 576.218 kb on - strandat 576.218 kb on - strandat 576.250 kb on - strandat 576.250 kb on - strandat 576.589 kb on - strand, within Psyr_0518at 579.001 kb on - strandat 579.001 kb on - strandat 579.137 kb on - strandat 579.142 kb on - strandat 579.142 kb on - strandat 579.318 kb on + strandat 579.319 kb on - strandat 579.556 kb on + strandat 579.556 kb on + strandat 579.557 kb on - strandat 579.557 kb on - strandat 579.639 kb on + strand, within Psyr_0519at 579.640 kb on - strand, within Psyr_0519at 579.640 kb on - strand, within Psyr_0519at 579.762 kb on - strand, within Psyr_0519at 579.762 kb on - strand, within Psyr_0519at 579.773 kb on + strand, within Psyr_0519at 579.773 kb on + strand, within Psyr_0519at 579.773 kb on + strand, within Psyr_0519at 579.773 kb on + strand, within Psyr_0519at 579.774 kb on - strand, within Psyr_0519

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Per-strain Table

Position Strand Gene LocusTag Fraction no stress control
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576,190 - -3.8
576,190 - -1.0
576,218 - +2.9
576,218 - -1.6
576,250 - +0.4
576,250 - -0.5
576,589 - Psyr_0518 0.12 +1.3
579,001 - -0.3
579,001 - -0.3
579,137 - -0.0
579,142 - +1.1
579,142 - +0.1
579,318 + +2.3
579,319 - +1.1
579,556 + -0.3
579,556 + -0.6
579,557 - +0.1
579,557 - +0.2
579,639 + Psyr_0519 0.11 +0.3
579,640 - Psyr_0519 0.11 -1.8
579,640 - Psyr_0519 0.11 -3.2
579,762 - Psyr_0519 0.15 -0.2
579,762 - Psyr_0519 0.15 -0.5
579,773 + Psyr_0519 0.16 -0.4
579,773 + Psyr_0519 0.16 -2.6
579,773 + Psyr_0519 0.16 +0.6
579,773 + Psyr_0519 0.16 +1.6
579,774 - Psyr_0519 0.16 -0.7

Or see this region's nucleotide sequence