Experiment: M9 with PEG 6000 16 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_004806 and ABIE53_004807 overlap by 4 nucleotides ABIE53_004807 and ABIE53_004808 overlap by 25 nucleotides ABIE53_004808 and ABIE53_004809 overlap by 4 nucleotides
ABIE53_004806: ABIE53_004806 - pilus assembly protein FimV, at 1,011,722 to 1,014,253
_004806
ABIE53_004807: ABIE53_004807 - tRNA pseudouridine38-40 synthase, at 1,014,250 to 1,015,062
_004807
ABIE53_004808: ABIE53_004808 - phosphoribosylanthranilate isomerase, at 1,015,038 to 1,015,781
_004808
ABIE53_004809: ABIE53_004809 - tryptophan synthase beta chain, at 1,015,778 to 1,017,031
_004809
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1013.367 kb on + strand, within ABIE53_004806 at 1013.368 kb on - strand, within ABIE53_004806 at 1013.553 kb on + strand, within ABIE53_004806 at 1013.554 kb on - strand, within ABIE53_004806 at 1013.785 kb on + strand, within ABIE53_004806 at 1013.786 kb on - strand, within ABIE53_004806 at 1014.132 kb on - strand at 1014.132 kb on - strand at 1014.132 kb on - strand at 1014.267 kb on - strand at 1014.278 kb on - strand at 1014.381 kb on + strand, within ABIE53_004807 at 1014.381 kb on + strand, within ABIE53_004807 at 1014.382 kb on - strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.577 kb on + strand, within ABIE53_004807 at 1014.578 kb on - strand, within ABIE53_004807 at 1014.578 kb on - strand, within ABIE53_004807 at 1014.578 kb on - strand, within ABIE53_004807 at 1014.578 kb on - strand, within ABIE53_004807 at 1014.578 kb on - strand, within ABIE53_004807 at 1014.578 kb on - strand, within ABIE53_004807 at 1014.578 kb on - strand, within ABIE53_004807 at 1014.578 kb on - strand, within ABIE53_004807 at 1014.580 kb on + strand, within ABIE53_004807 at 1014.580 kb on + strand, within ABIE53_004807 at 1014.580 kb on + strand, within ABIE53_004807 at 1014.580 kb on + strand, within ABIE53_004807 at 1014.580 kb on + strand, within ABIE53_004807 at 1014.580 kb on + strand, within ABIE53_004807 at 1014.581 kb on - strand, within ABIE53_004807 at 1014.581 kb on - strand, within ABIE53_004807 at 1014.583 kb on + strand, within ABIE53_004807 at 1014.583 kb on + strand, within ABIE53_004807 at 1014.583 kb on + strand, within ABIE53_004807 at 1014.592 kb on + strand, within ABIE53_004807 at 1014.592 kb on + strand, within ABIE53_004807 at 1014.592 kb on + strand, within ABIE53_004807 at 1014.593 kb on - strand, within ABIE53_004807 at 1014.593 kb on - strand, within ABIE53_004807 at 1014.862 kb on + strand, within ABIE53_004807 at 1014.863 kb on - strand, within ABIE53_004807 at 1014.863 kb on - strand, within ABIE53_004807 at 1014.863 kb on - strand, within ABIE53_004807 at 1014.863 kb on - strand, within ABIE53_004807 at 1014.863 kb on - strand, within ABIE53_004807 at 1014.863 kb on - strand, within ABIE53_004807 at 1014.863 kb on - strand, within ABIE53_004807 at 1014.961 kb on + strand, within ABIE53_004807 at 1014.961 kb on + strand, within ABIE53_004807 at 1014.961 kb on + strand, within ABIE53_004807 at 1014.979 kb on + strand, within ABIE53_004807 at 1015.037 kb on + strand at 1015.037 kb on + strand at 1015.037 kb on + strand at 1015.583 kb on + strand, within ABIE53_004808
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with PEG 6000 16 vol% remove 1,013,367 + ABIE53_004806 0.65 +0.2 1,013,368 - ABIE53_004806 0.65 -0.6 1,013,553 + ABIE53_004806 0.72 -1.0 1,013,554 - ABIE53_004806 0.72 +0.1 1,013,785 + ABIE53_004806 0.81 -2.4 1,013,786 - ABIE53_004806 0.82 -0.6 1,014,132 - -1.6 1,014,132 - -0.9 1,014,132 - -0.3 1,014,267 - -0.7 1,014,278 - -2.2 1,014,381 + ABIE53_004807 0.16 +1.6 1,014,381 + ABIE53_004807 0.16 -0.7 1,014,382 - ABIE53_004807 0.16 -0.2 1,014,577 + ABIE53_004807 0.40 +0.1 1,014,577 + ABIE53_004807 0.40 -0.3 1,014,577 + ABIE53_004807 0.40 +0.3 1,014,577 + ABIE53_004807 0.40 -0.6 1,014,577 + ABIE53_004807 0.40 -0.5 1,014,577 + ABIE53_004807 0.40 -1.7 1,014,577 + ABIE53_004807 0.40 -0.8 1,014,577 + ABIE53_004807 0.40 -0.5 1,014,577 + ABIE53_004807 0.40 -0.5 1,014,578 - ABIE53_004807 0.40 +0.0 1,014,578 - ABIE53_004807 0.40 -0.7 1,014,578 - ABIE53_004807 0.40 +2.1 1,014,578 - ABIE53_004807 0.40 -0.7 1,014,578 - ABIE53_004807 0.40 -1.0 1,014,578 - ABIE53_004807 0.40 -0.5 1,014,578 - ABIE53_004807 0.40 -0.1 1,014,578 - ABIE53_004807 0.40 -0.7 1,014,580 + ABIE53_004807 0.41 -0.2 1,014,580 + ABIE53_004807 0.41 -1.2 1,014,580 + ABIE53_004807 0.41 -0.5 1,014,580 + ABIE53_004807 0.41 -0.3 1,014,580 + ABIE53_004807 0.41 +0.1 1,014,580 + ABIE53_004807 0.41 +0.5 1,014,581 - ABIE53_004807 0.41 +1.1 1,014,581 - ABIE53_004807 0.41 +0.3 1,014,583 + ABIE53_004807 0.41 -0.4 1,014,583 + ABIE53_004807 0.41 -0.8 1,014,583 + ABIE53_004807 0.41 +0.1 1,014,592 + ABIE53_004807 0.42 +2.4 1,014,592 + ABIE53_004807 0.42 -0.4 1,014,592 + ABIE53_004807 0.42 -0.1 1,014,593 - ABIE53_004807 0.42 -0.9 1,014,593 - ABIE53_004807 0.42 -1.4 1,014,862 + ABIE53_004807 0.75 +0.8 1,014,863 - ABIE53_004807 0.75 +2.4 1,014,863 - ABIE53_004807 0.75 -0.4 1,014,863 - ABIE53_004807 0.75 -0.5 1,014,863 - ABIE53_004807 0.75 -0.5 1,014,863 - ABIE53_004807 0.75 -0.8 1,014,863 - ABIE53_004807 0.75 +0.7 1,014,863 - ABIE53_004807 0.75 -0.2 1,014,961 + ABIE53_004807 0.87 +0.2 1,014,961 + ABIE53_004807 0.87 -0.5 1,014,961 + ABIE53_004807 0.87 +0.1 1,014,979 + ABIE53_004807 0.90 -0.6 1,015,037 + +0.7 1,015,037 + -0.7 1,015,037 + -0.5 1,015,583 + ABIE53_004808 0.73 -0.2
Or see this region's nucleotide sequence