Experiment: R2A with PEG 6000 16 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_002126 and ABIE53_002127 are separated by 141 nucleotides ABIE53_002127 and ABIE53_002128 are separated by 103 nucleotides ABIE53_002128 and ABIE53_002129 are separated by 9 nucleotides ABIE53_002129 and ABIE53_002130 are separated by 50 nucleotides
ABIE53_002126: ABIE53_002126 - type II secretory pathway pseudopilin PulG, at 2,382,393 to 2,382,668
_002126
ABIE53_002127: ABIE53_002127 - hypothetical protein, at 2,382,810 to 2,383,334
_002127
ABIE53_002128: ABIE53_002128 - hypothetical protein, at 2,383,438 to 2,384,808
_002128
ABIE53_002129: ABIE53_002129 - meso-butanediol dehydrogenase/(S,S)-butanediol dehydrogenase/diacetyl reductase, at 2,384,818 to 2,385,675
_002129
ABIE53_002130: ABIE53_002130 - flavin reductase (DIM6/NTAB) family NADH-FMN oxidoreductase RutF, at 2,385,726 to 2,386,229
_002130
Position (kb)
2383
2384
2385 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2382.444 kb on - strand, within ABIE53_002126 at 2382.528 kb on + strand, within ABIE53_002126 at 2382.529 kb on - strand, within ABIE53_002126 at 2382.529 kb on - strand, within ABIE53_002126 at 2382.530 kb on + strand, within ABIE53_002126 at 2382.530 kb on + strand, within ABIE53_002126 at 2382.675 kb on + strand at 2382.676 kb on - strand at 2382.676 kb on - strand at 2382.688 kb on + strand at 2382.688 kb on + strand at 2382.689 kb on - strand at 2382.751 kb on + strand at 2382.812 kb on - strand at 2382.828 kb on - strand at 2382.867 kb on + strand, within ABIE53_002127 at 2382.869 kb on + strand, within ABIE53_002127 at 2382.928 kb on + strand, within ABIE53_002127 at 2382.955 kb on + strand, within ABIE53_002127 at 2382.955 kb on + strand, within ABIE53_002127 at 2382.955 kb on + strand, within ABIE53_002127 at 2382.955 kb on + strand, within ABIE53_002127 at 2382.956 kb on - strand, within ABIE53_002127 at 2382.956 kb on - strand, within ABIE53_002127 at 2383.051 kb on + strand, within ABIE53_002127 at 2383.051 kb on + strand, within ABIE53_002127 at 2383.136 kb on + strand, within ABIE53_002127 at 2383.137 kb on - strand, within ABIE53_002127 at 2383.218 kb on + strand, within ABIE53_002127 at 2383.219 kb on - strand, within ABIE53_002127 at 2383.219 kb on - strand, within ABIE53_002127 at 2383.222 kb on + strand, within ABIE53_002127 at 2383.222 kb on + strand, within ABIE53_002127 at 2383.223 kb on - strand, within ABIE53_002127 at 2383.223 kb on - strand, within ABIE53_002127 at 2383.269 kb on - strand, within ABIE53_002127 at 2383.302 kb on + strand at 2383.302 kb on + strand at 2383.303 kb on - strand at 2383.394 kb on + strand at 2383.395 kb on - strand at 2383.399 kb on + strand at 2383.400 kb on - strand at 2383.400 kb on - strand at 2383.400 kb on - strand at 2383.424 kb on - strand at 2383.524 kb on - strand at 2383.556 kb on - strand at 2383.576 kb on + strand, within ABIE53_002128 at 2383.577 kb on - strand, within ABIE53_002128 at 2383.577 kb on - strand, within ABIE53_002128 at 2383.577 kb on - strand, within ABIE53_002128 at 2383.577 kb on - strand, within ABIE53_002128 at 2383.577 kb on - strand, within ABIE53_002128 at 2383.656 kb on + strand, within ABIE53_002128 at 2383.712 kb on + strand, within ABIE53_002128 at 2383.712 kb on + strand, within ABIE53_002128 at 2383.712 kb on + strand, within ABIE53_002128 at 2383.713 kb on - strand, within ABIE53_002128 at 2383.713 kb on - strand, within ABIE53_002128 at 2383.713 kb on - strand, within ABIE53_002128 at 2383.713 kb on - strand, within ABIE53_002128 at 2383.915 kb on + strand, within ABIE53_002128 at 2383.915 kb on + strand, within ABIE53_002128 at 2383.915 kb on + strand, within ABIE53_002128 at 2383.916 kb on - strand, within ABIE53_002128 at 2383.916 kb on - strand, within ABIE53_002128 at 2383.916 kb on - strand, within ABIE53_002128 at 2383.916 kb on - strand, within ABIE53_002128 at 2383.916 kb on - strand, within ABIE53_002128 at 2383.916 kb on - strand, within ABIE53_002128 at 2383.916 kb on - strand, within ABIE53_002128 at 2383.916 kb on - strand, within ABIE53_002128 at 2384.043 kb on - strand, within ABIE53_002128 at 2384.086 kb on + strand, within ABIE53_002128 at 2384.087 kb on - strand, within ABIE53_002128 at 2384.516 kb on + strand, within ABIE53_002128 at 2384.517 kb on - strand, within ABIE53_002128 at 2385.321 kb on + strand, within ABIE53_002129 at 2385.321 kb on + strand, within ABIE53_002129 at 2385.582 kb on - strand, within ABIE53_002129 at 2385.582 kb on - strand, within ABIE53_002129 at 2385.582 kb on - strand, within ABIE53_002129 at 2385.620 kb on + strand at 2385.685 kb on + strand at 2385.711 kb on + strand at 2385.711 kb on + strand at 2385.711 kb on + strand at 2385.712 kb on - strand at 2385.712 kb on - strand at 2385.774 kb on + strand at 2385.774 kb on + strand at 2385.774 kb on + strand at 2385.774 kb on + strand at 2385.774 kb on + strand at 2385.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with PEG 6000 16 vol% remove 2,382,444 - ABIE53_002126 0.18 -0.6 2,382,528 + ABIE53_002126 0.49 +0.4 2,382,529 - ABIE53_002126 0.49 -0.2 2,382,529 - ABIE53_002126 0.49 +0.6 2,382,530 + ABIE53_002126 0.50 +1.0 2,382,530 + ABIE53_002126 0.50 -0.1 2,382,675 + +0.5 2,382,676 - -0.7 2,382,676 - +1.0 2,382,688 + +0.1 2,382,688 + -0.1 2,382,689 - +1.7 2,382,751 + +1.6 2,382,812 - +1.5 2,382,828 - +0.1 2,382,867 + ABIE53_002127 0.11 +0.7 2,382,869 + ABIE53_002127 0.11 -3.1 2,382,928 + ABIE53_002127 0.22 +0.3 2,382,955 + ABIE53_002127 0.28 -0.2 2,382,955 + ABIE53_002127 0.28 -0.3 2,382,955 + ABIE53_002127 0.28 -0.7 2,382,955 + ABIE53_002127 0.28 +1.5 2,382,956 - ABIE53_002127 0.28 +0.1 2,382,956 - ABIE53_002127 0.28 -1.3 2,383,051 + ABIE53_002127 0.46 +0.9 2,383,051 + ABIE53_002127 0.46 +0.5 2,383,136 + ABIE53_002127 0.62 +1.3 2,383,137 - ABIE53_002127 0.62 +0.4 2,383,218 + ABIE53_002127 0.78 +0.3 2,383,219 - ABIE53_002127 0.78 +0.3 2,383,219 - ABIE53_002127 0.78 +0.5 2,383,222 + ABIE53_002127 0.78 +0.5 2,383,222 + ABIE53_002127 0.78 +1.4 2,383,223 - ABIE53_002127 0.79 +0.7 2,383,223 - ABIE53_002127 0.79 +2.7 2,383,269 - ABIE53_002127 0.87 -0.4 2,383,302 + -0.9 2,383,302 + -0.6 2,383,303 - -0.7 2,383,394 + +0.7 2,383,395 - +1.1 2,383,399 + +0.5 2,383,400 - -1.3 2,383,400 - +0.1 2,383,400 - +1.0 2,383,424 - +0.5 2,383,524 - -1.5 2,383,556 - -0.3 2,383,576 + ABIE53_002128 0.10 -0.9 2,383,577 - ABIE53_002128 0.10 +0.6 2,383,577 - ABIE53_002128 0.10 -0.4 2,383,577 - ABIE53_002128 0.10 -0.5 2,383,577 - ABIE53_002128 0.10 +1.1 2,383,577 - ABIE53_002128 0.10 +1.7 2,383,656 + ABIE53_002128 0.16 +1.8 2,383,712 + ABIE53_002128 0.20 -0.9 2,383,712 + ABIE53_002128 0.20 -0.4 2,383,712 + ABIE53_002128 0.20 +0.5 2,383,713 - ABIE53_002128 0.20 -0.5 2,383,713 - ABIE53_002128 0.20 +0.0 2,383,713 - ABIE53_002128 0.20 +0.3 2,383,713 - ABIE53_002128 0.20 -0.6 2,383,915 + ABIE53_002128 0.35 +2.1 2,383,915 + ABIE53_002128 0.35 -0.4 2,383,915 + ABIE53_002128 0.35 +0.4 2,383,916 - ABIE53_002128 0.35 -1.0 2,383,916 - ABIE53_002128 0.35 +0.2 2,383,916 - ABIE53_002128 0.35 -0.7 2,383,916 - ABIE53_002128 0.35 +0.1 2,383,916 - ABIE53_002128 0.35 -2.5 2,383,916 - ABIE53_002128 0.35 -0.3 2,383,916 - ABIE53_002128 0.35 +0.6 2,383,916 - ABIE53_002128 0.35 +0.3 2,384,043 - ABIE53_002128 0.44 -1.5 2,384,086 + ABIE53_002128 0.47 -0.2 2,384,087 - ABIE53_002128 0.47 -1.5 2,384,516 + ABIE53_002128 0.79 +0.6 2,384,517 - ABIE53_002128 0.79 -1.6 2,385,321 + ABIE53_002129 0.59 -0.7 2,385,321 + ABIE53_002129 0.59 -1.9 2,385,582 - ABIE53_002129 0.89 -1.7 2,385,582 - ABIE53_002129 0.89 +2.7 2,385,582 - ABIE53_002129 0.89 -1.6 2,385,620 + -0.2 2,385,685 + -0.9 2,385,711 + -0.3 2,385,711 + -0.5 2,385,711 + +0.3 2,385,712 - +0.5 2,385,712 - -2.3 2,385,774 + -0.7 2,385,774 + -0.7 2,385,774 + +0.5 2,385,774 + +0.8 2,385,774 + -0.7 2,385,775 - +0.2
Or see this region's nucleotide sequence