Experiment: M9 with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_004909 and ABIE53_004910 are separated by 18 nucleotides ABIE53_004910 and ABIE53_004911 are separated by 185 nucleotides ABIE53_004911 and ABIE53_004912 are separated by 165 nucleotides
ABIE53_004909: ABIE53_004909 - sugar phosphate permease, at 1,127,286 to 1,128,605
_004909
ABIE53_004910: ABIE53_004910 - hypothetical protein, at 1,128,624 to 1,128,851
_004910
ABIE53_004911: ABIE53_004911 - type IV secretory pathway VirB2 component (pilin), at 1,129,037 to 1,129,243
_004911
ABIE53_004912: ABIE53_004912 - hypothetical protein, at 1,129,409 to 1,129,582
_004912
Position (kb) 1128
1129 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1127.821 kb on + strand, within ABIE53_004909 at 1127.842 kb on + strand, within ABIE53_004909 at 1127.842 kb on + strand, within ABIE53_004909 at 1127.843 kb on - strand, within ABIE53_004909 at 1127.843 kb on - strand, within ABIE53_004909 at 1127.843 kb on - strand, within ABIE53_004909 at 1127.843 kb on - strand, within ABIE53_004909 at 1127.843 kb on - strand, within ABIE53_004909 at 1127.858 kb on - strand, within ABIE53_004909 at 1127.858 kb on - strand, within ABIE53_004909 at 1127.858 kb on - strand, within ABIE53_004909 at 1127.858 kb on - strand, within ABIE53_004909 at 1127.952 kb on + strand, within ABIE53_004909 at 1127.953 kb on - strand, within ABIE53_004909 at 1127.953 kb on - strand, within ABIE53_004909 at 1127.977 kb on + strand, within ABIE53_004909 at 1127.978 kb on - strand, within ABIE53_004909 at 1127.978 kb on - strand, within ABIE53_004909 at 1127.978 kb on - strand, within ABIE53_004909 at 1128.022 kb on + strand, within ABIE53_004909 at 1128.022 kb on + strand, within ABIE53_004909 at 1128.023 kb on - strand, within ABIE53_004909 at 1128.403 kb on + strand, within ABIE53_004909 at 1128.403 kb on + strand, within ABIE53_004909 at 1128.403 kb on + strand, within ABIE53_004909 at 1128.403 kb on + strand, within ABIE53_004909 at 1128.403 kb on + strand, within ABIE53_004909 at 1128.403 kb on + strand, within ABIE53_004909 at 1128.508 kb on + strand at 1128.508 kb on + strand at 1128.508 kb on + strand at 1128.508 kb on + strand at 1128.508 kb on + strand at 1128.508 kb on + strand at 1128.509 kb on - strand at 1128.509 kb on - strand at 1128.509 kb on - strand at 1128.509 kb on - strand at 1128.509 kb on - strand at 1128.509 kb on - strand at 1128.509 kb on - strand at 1128.758 kb on - strand, within ABIE53_004910 at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.830 kb on + strand at 1128.831 kb on - strand at 1128.831 kb on - strand at 1128.831 kb on - strand at 1128.831 kb on - strand at 1128.831 kb on - strand at 1128.831 kb on - strand at 1128.831 kb on - strand at 1128.960 kb on - strand at 1128.962 kb on + strand at 1128.962 kb on + strand at 1128.962 kb on + strand at 1128.962 kb on + strand at 1128.962 kb on + strand at 1128.963 kb on - strand at 1128.963 kb on - strand at 1128.963 kb on - strand at 1128.963 kb on - strand at 1128.963 kb on - strand at 1128.963 kb on - strand at 1128.996 kb on - strand at 1129.025 kb on - strand at 1129.025 kb on - strand at 1129.025 kb on - strand at 1129.057 kb on + strand at 1129.059 kb on + strand, within ABIE53_004911 at 1129.059 kb on + strand, within ABIE53_004911 at 1129.059 kb on + strand, within ABIE53_004911 at 1129.059 kb on + strand, within ABIE53_004911 at 1129.059 kb on + strand, within ABIE53_004911 at 1129.059 kb on + strand, within ABIE53_004911 at 1129.059 kb on + strand, within ABIE53_004911 at 1129.059 kb on + strand, within ABIE53_004911 at 1129.059 kb on + strand, within ABIE53_004911 at 1129.060 kb on - strand, within ABIE53_004911 at 1129.060 kb on - strand, within ABIE53_004911 at 1129.060 kb on - strand, within ABIE53_004911 at 1129.060 kb on - strand, within ABIE53_004911 at 1129.060 kb on - strand, within ABIE53_004911 at 1129.060 kb on - strand, within ABIE53_004911 at 1129.211 kb on - strand, within ABIE53_004911 at 1129.242 kb on - strand at 1129.301 kb on + strand at 1129.301 kb on + strand at 1129.302 kb on - strand at 1129.302 kb on - strand at 1129.356 kb on + strand at 1129.580 kb on + strand at 1129.580 kb on + strand at 1129.580 kb on + strand at 1129.580 kb on + strand at 1129.581 kb on - strand at 1129.581 kb on - strand at 1129.581 kb on - strand at 1129.581 kb on - strand at 1129.581 kb on - strand at 1129.581 kb on - strand at 1129.616 kb on + strand at 1129.616 kb on + strand at 1129.616 kb on + strand at 1129.616 kb on + strand at 1129.617 kb on - strand at 1129.617 kb on - strand at 1129.617 kb on - strand at 1129.617 kb on - strand at 1129.647 kb on - strand at 1129.647 kb on - strand at 1129.647 kb on - strand at 1129.651 kb on + strand at 1129.651 kb on + strand at 1129.651 kb on + strand at 1129.651 kb on + strand at 1129.651 kb on + strand at 1129.651 kb on + strand at 1129.652 kb on - strand at 1129.652 kb on - strand at 1129.762 kb on + strand at 1129.762 kb on + strand at 1129.762 kb on + strand at 1129.762 kb on + strand at 1129.762 kb on + strand at 1129.762 kb on + strand at 1129.762 kb on + strand at 1129.763 kb on - strand at 1129.763 kb on - strand at 1129.763 kb on - strand at 1129.763 kb on - strand at 1129.763 kb on - strand at 1129.773 kb on + strand at 1129.773 kb on + strand at 1129.773 kb on + strand at 1129.773 kb on + strand at 1129.773 kb on + strand at 1129.773 kb on + strand at 1129.774 kb on - strand at 1129.786 kb on + strand at 1129.786 kb on + strand at 1129.787 kb on - strand at 1129.787 kb on - strand at 1129.787 kb on - strand at 1129.787 kb on - strand at 1129.819 kb on + strand at 1129.820 kb on - strand at 1129.820 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Chloride 200 mM remove 1,127,821 + ABIE53_004909 0.41 -1.2 1,127,842 + ABIE53_004909 0.42 -2.0 1,127,842 + ABIE53_004909 0.42 +0.7 1,127,843 - ABIE53_004909 0.42 +0.5 1,127,843 - ABIE53_004909 0.42 -0.9 1,127,843 - ABIE53_004909 0.42 +0.4 1,127,843 - ABIE53_004909 0.42 -0.3 1,127,843 - ABIE53_004909 0.42 -1.8 1,127,858 - ABIE53_004909 0.43 -0.7 1,127,858 - ABIE53_004909 0.43 +0.1 1,127,858 - ABIE53_004909 0.43 -1.0 1,127,858 - ABIE53_004909 0.43 -0.2 1,127,952 + ABIE53_004909 0.50 +0.3 1,127,953 - ABIE53_004909 0.51 -1.1 1,127,953 - ABIE53_004909 0.51 +1.0 1,127,977 + ABIE53_004909 0.52 -2.2 1,127,978 - ABIE53_004909 0.52 +1.3 1,127,978 - ABIE53_004909 0.52 -0.4 1,127,978 - ABIE53_004909 0.52 +1.2 1,128,022 + ABIE53_004909 0.56 +1.4 1,128,022 + ABIE53_004909 0.56 -0.7 1,128,023 - ABIE53_004909 0.56 -0.1 1,128,403 + ABIE53_004909 0.85 -1.8 1,128,403 + ABIE53_004909 0.85 -0.9 1,128,403 + ABIE53_004909 0.85 -2.2 1,128,403 + ABIE53_004909 0.85 +1.3 1,128,403 + ABIE53_004909 0.85 +0.9 1,128,403 + ABIE53_004909 0.85 -0.0 1,128,508 + +1.6 1,128,508 + +1.2 1,128,508 + -0.3 1,128,508 + -0.7 1,128,508 + -1.0 1,128,508 + +0.2 1,128,509 - -3.2 1,128,509 - +0.6 1,128,509 - +0.3 1,128,509 - -1.2 1,128,509 - -0.0 1,128,509 - +0.2 1,128,509 - -1.4 1,128,758 - ABIE53_004910 0.59 -0.5 1,128,830 + +0.9 1,128,830 + +2.6 1,128,830 + -0.6 1,128,830 + +0.2 1,128,830 + -1.2 1,128,830 + +0.4 1,128,830 + -1.4 1,128,830 + +0.4 1,128,830 + +1.0 1,128,830 + -2.2 1,128,831 - +0.2 1,128,831 - -0.6 1,128,831 - -1.8 1,128,831 - -0.1 1,128,831 - +1.4 1,128,831 - -1.0 1,128,831 - +0.6 1,128,960 - -0.8 1,128,962 + -0.4 1,128,962 + +0.1 1,128,962 + +2.9 1,128,962 + -0.3 1,128,962 + -0.6 1,128,963 - -1.3 1,128,963 - +0.1 1,128,963 - -0.3 1,128,963 - -1.2 1,128,963 - -0.8 1,128,963 - -1.1 1,128,996 - -1.0 1,129,025 - -1.3 1,129,025 - +0.2 1,129,025 - -0.1 1,129,057 + -1.4 1,129,059 + ABIE53_004911 0.11 -0.4 1,129,059 + ABIE53_004911 0.11 -1.4 1,129,059 + ABIE53_004911 0.11 +0.2 1,129,059 + ABIE53_004911 0.11 +0.0 1,129,059 + ABIE53_004911 0.11 +0.2 1,129,059 + ABIE53_004911 0.11 +0.6 1,129,059 + ABIE53_004911 0.11 +0.0 1,129,059 + ABIE53_004911 0.11 +1.6 1,129,059 + ABIE53_004911 0.11 +0.6 1,129,060 - ABIE53_004911 0.11 +0.9 1,129,060 - ABIE53_004911 0.11 -0.7 1,129,060 - ABIE53_004911 0.11 +0.1 1,129,060 - ABIE53_004911 0.11 -1.0 1,129,060 - ABIE53_004911 0.11 -0.7 1,129,060 - ABIE53_004911 0.11 +0.1 1,129,211 - ABIE53_004911 0.84 -0.9 1,129,242 - -1.1 1,129,301 + +0.3 1,129,301 + -0.8 1,129,302 - -2.9 1,129,302 - -1.6 1,129,356 + -0.5 1,129,580 + +3.2 1,129,580 + +0.4 1,129,580 + -0.6 1,129,580 + -1.1 1,129,581 - -0.4 1,129,581 - -1.5 1,129,581 - +1.9 1,129,581 - +0.3 1,129,581 - -0.1 1,129,581 - +0.3 1,129,616 + -0.4 1,129,616 + -0.2 1,129,616 + +0.2 1,129,616 + +0.6 1,129,617 - -0.5 1,129,617 - -0.5 1,129,617 - +0.5 1,129,617 - -1.1 1,129,647 - -1.4 1,129,647 - -0.3 1,129,647 - -0.5 1,129,651 + +1.3 1,129,651 + +0.6 1,129,651 + +0.5 1,129,651 + -1.1 1,129,651 + +0.0 1,129,651 + +0.4 1,129,652 - +0.2 1,129,652 - -0.2 1,129,762 + -2.0 1,129,762 + -0.2 1,129,762 + -1.5 1,129,762 + -1.0 1,129,762 + -0.9 1,129,762 + -1.2 1,129,762 + -0.7 1,129,763 - -0.3 1,129,763 - -0.4 1,129,763 - -0.5 1,129,763 - -0.4 1,129,763 - -2.0 1,129,773 + +1.9 1,129,773 + -0.3 1,129,773 + +0.4 1,129,773 + -0.5 1,129,773 + -1.3 1,129,773 + -1.1 1,129,774 - +0.4 1,129,786 + -0.2 1,129,786 + -0.3 1,129,787 - +0.3 1,129,787 - -1.0 1,129,787 - +0.0 1,129,787 - +0.6 1,129,819 + -1.7 1,129,820 - -2.2 1,129,820 - -1.5
Or see this region's nucleotide sequence