Experiment: M9 with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000575 and ABIE53_000576 are separated by 125 nucleotides ABIE53_000576 and ABIE53_000577 are separated by 6 nucleotides
ABIE53_000575: ABIE53_000575 - putative alpha/beta hydrolase family esterase, at 625,613 to 626,215
_000575
ABIE53_000576: ABIE53_000576 - sulfate transport system substrate-binding protein, at 626,341 to 627,372
_000576
ABIE53_000577: ABIE53_000577 - biotin synthase, at 627,379 to 628,473
_000577
Position (kb)
626
627
628 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 625.435 kb on + strand at 625.435 kb on + strand at 625.436 kb on - strand at 625.436 kb on - strand at 625.436 kb on - strand at 625.453 kb on + strand at 625.454 kb on - strand at 625.880 kb on + strand, within ABIE53_000575 at 625.880 kb on + strand, within ABIE53_000575 at 625.881 kb on - strand, within ABIE53_000575 at 626.129 kb on + strand, within ABIE53_000575 at 626.130 kb on - strand, within ABIE53_000575 at 626.130 kb on - strand, within ABIE53_000575 at 626.130 kb on - strand, within ABIE53_000575 at 626.130 kb on - strand, within ABIE53_000575 at 626.130 kb on - strand, within ABIE53_000575 at 626.130 kb on - strand, within ABIE53_000575 at 626.214 kb on - strand at 626.261 kb on + strand at 626.261 kb on + strand at 626.261 kb on + strand at 626.261 kb on + strand at 626.261 kb on + strand at 626.261 kb on + strand at 626.261 kb on + strand at 626.262 kb on - strand at 626.262 kb on - strand at 626.262 kb on - strand at 626.262 kb on - strand at 626.262 kb on - strand at 626.262 kb on - strand at 626.263 kb on + strand at 626.264 kb on - strand at 626.264 kb on - strand at 626.320 kb on - strand at 626.420 kb on + strand at 626.462 kb on - strand, within ABIE53_000576 at 626.462 kb on - strand, within ABIE53_000576 at 626.468 kb on + strand, within ABIE53_000576 at 626.468 kb on + strand, within ABIE53_000576 at 626.469 kb on - strand, within ABIE53_000576 at 626.469 kb on - strand, within ABIE53_000576 at 626.482 kb on + strand, within ABIE53_000576 at 626.482 kb on + strand, within ABIE53_000576 at 626.483 kb on - strand, within ABIE53_000576 at 626.483 kb on - strand, within ABIE53_000576 at 626.483 kb on - strand, within ABIE53_000576 at 626.515 kb on + strand, within ABIE53_000576 at 626.515 kb on + strand, within ABIE53_000576 at 626.516 kb on - strand, within ABIE53_000576 at 626.516 kb on - strand, within ABIE53_000576 at 626.516 kb on - strand, within ABIE53_000576 at 626.516 kb on - strand, within ABIE53_000576 at 626.517 kb on + strand, within ABIE53_000576 at 626.517 kb on + strand, within ABIE53_000576 at 626.517 kb on + strand, within ABIE53_000576 at 626.517 kb on + strand, within ABIE53_000576 at 626.518 kb on - strand, within ABIE53_000576 at 626.518 kb on - strand, within ABIE53_000576 at 626.518 kb on - strand, within ABIE53_000576 at 626.588 kb on + strand, within ABIE53_000576 at 626.713 kb on + strand, within ABIE53_000576 at 626.839 kb on + strand, within ABIE53_000576 at 626.840 kb on - strand, within ABIE53_000576 at 626.845 kb on + strand, within ABIE53_000576 at 626.845 kb on + strand, within ABIE53_000576 at 626.845 kb on + strand, within ABIE53_000576 at 626.845 kb on + strand, within ABIE53_000576 at 626.845 kb on + strand, within ABIE53_000576 at 626.845 kb on + strand, within ABIE53_000576 at 626.846 kb on - strand, within ABIE53_000576 at 626.846 kb on - strand, within ABIE53_000576 at 626.846 kb on - strand, within ABIE53_000576 at 626.846 kb on - strand, within ABIE53_000576 at 627.067 kb on + strand, within ABIE53_000576 at 627.067 kb on + strand, within ABIE53_000576 at 627.068 kb on - strand, within ABIE53_000576 at 627.157 kb on + strand, within ABIE53_000576 at 627.157 kb on + strand, within ABIE53_000576 at 627.158 kb on - strand, within ABIE53_000576 at 627.158 kb on - strand, within ABIE53_000576 at 627.158 kb on - strand, within ABIE53_000576 at 627.158 kb on - strand, within ABIE53_000576 at 627.166 kb on + strand, within ABIE53_000576 at 627.166 kb on + strand, within ABIE53_000576 at 627.167 kb on - strand, within ABIE53_000576 at 627.167 kb on - strand, within ABIE53_000576 at 627.167 kb on - strand, within ABIE53_000576 at 627.167 kb on - strand, within ABIE53_000576 at 627.370 kb on + strand at 627.370 kb on + strand at 627.370 kb on + strand at 627.370 kb on + strand at 627.370 kb on + strand at 627.371 kb on - strand at 627.371 kb on - strand at 627.371 kb on - strand at 627.371 kb on - strand at 627.371 kb on - strand at 627.371 kb on - strand at 627.380 kb on + strand at 627.380 kb on + strand at 627.381 kb on - strand at 627.522 kb on - strand, within ABIE53_000577 at 627.869 kb on + strand, within ABIE53_000577 at 627.869 kb on + strand, within ABIE53_000577 at 627.870 kb on - strand, within ABIE53_000577 at 627.912 kb on + strand, within ABIE53_000577 at 627.913 kb on - strand, within ABIE53_000577 at 627.913 kb on - strand, within ABIE53_000577 at 627.929 kb on + strand, within ABIE53_000577 at 627.929 kb on + strand, within ABIE53_000577 at 627.929 kb on + strand, within ABIE53_000577 at 627.929 kb on + strand, within ABIE53_000577 at 627.929 kb on + strand, within ABIE53_000577 at 627.930 kb on - strand, within ABIE53_000577 at 627.930 kb on - strand, within ABIE53_000577 at 627.930 kb on - strand, within ABIE53_000577 at 627.932 kb on + strand, within ABIE53_000577 at 627.932 kb on + strand, within ABIE53_000577 at 627.932 kb on + strand, within ABIE53_000577 at 627.932 kb on + strand, within ABIE53_000577 at 627.933 kb on - strand, within ABIE53_000577 at 628.203 kb on - strand, within ABIE53_000577 at 628.331 kb on + strand, within ABIE53_000577 at 628.332 kb on - strand, within ABIE53_000577
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Chloride 200 mM remove 625,435 + +1.0 625,435 + -0.6 625,436 - -0.5 625,436 - -0.7 625,436 - -0.7 625,453 + -1.3 625,454 - -0.4 625,880 + ABIE53_000575 0.44 +1.4 625,880 + ABIE53_000575 0.44 -1.8 625,881 - ABIE53_000575 0.44 +0.0 626,129 + ABIE53_000575 0.86 +0.1 626,130 - ABIE53_000575 0.86 -0.4 626,130 - ABIE53_000575 0.86 +1.3 626,130 - ABIE53_000575 0.86 +0.1 626,130 - ABIE53_000575 0.86 +1.3 626,130 - ABIE53_000575 0.86 -0.2 626,130 - ABIE53_000575 0.86 -0.3 626,214 - +2.4 626,261 + -1.8 626,261 + -0.6 626,261 + -0.3 626,261 + -1.3 626,261 + -1.0 626,261 + -0.5 626,261 + +0.6 626,262 - -0.6 626,262 - -0.1 626,262 - -0.6 626,262 - -1.1 626,262 - -0.4 626,262 - -0.4 626,263 + +0.6 626,264 - -0.3 626,264 - +0.4 626,320 - -1.0 626,420 + -0.3 626,462 - ABIE53_000576 0.12 -0.5 626,462 - ABIE53_000576 0.12 -0.0 626,468 + ABIE53_000576 0.12 -0.2 626,468 + ABIE53_000576 0.12 +0.6 626,469 - ABIE53_000576 0.12 -1.4 626,469 - ABIE53_000576 0.12 +0.0 626,482 + ABIE53_000576 0.14 -1.4 626,482 + ABIE53_000576 0.14 -0.8 626,483 - ABIE53_000576 0.14 -0.4 626,483 - ABIE53_000576 0.14 +0.5 626,483 - ABIE53_000576 0.14 +0.6 626,515 + ABIE53_000576 0.17 +0.9 626,515 + ABIE53_000576 0.17 +1.4 626,516 - ABIE53_000576 0.17 -1.8 626,516 - ABIE53_000576 0.17 -0.7 626,516 - ABIE53_000576 0.17 -2.8 626,516 - ABIE53_000576 0.17 -0.6 626,517 + ABIE53_000576 0.17 -3.3 626,517 + ABIE53_000576 0.17 -0.4 626,517 + ABIE53_000576 0.17 -0.7 626,517 + ABIE53_000576 0.17 -0.3 626,518 - ABIE53_000576 0.17 -1.8 626,518 - ABIE53_000576 0.17 +3.0 626,518 - ABIE53_000576 0.17 +0.3 626,588 + ABIE53_000576 0.24 +2.0 626,713 + ABIE53_000576 0.36 +0.3 626,839 + ABIE53_000576 0.48 +0.3 626,840 - ABIE53_000576 0.48 +1.0 626,845 + ABIE53_000576 0.49 -1.1 626,845 + ABIE53_000576 0.49 +1.2 626,845 + ABIE53_000576 0.49 -0.4 626,845 + ABIE53_000576 0.49 -1.0 626,845 + ABIE53_000576 0.49 -0.4 626,845 + ABIE53_000576 0.49 -0.4 626,846 - ABIE53_000576 0.49 +1.0 626,846 - ABIE53_000576 0.49 +0.7 626,846 - ABIE53_000576 0.49 -1.8 626,846 - ABIE53_000576 0.49 -0.4 627,067 + ABIE53_000576 0.70 -0.0 627,067 + ABIE53_000576 0.70 -1.4 627,068 - ABIE53_000576 0.70 +0.4 627,157 + ABIE53_000576 0.79 -1.7 627,157 + ABIE53_000576 0.79 -0.5 627,158 - ABIE53_000576 0.79 +0.4 627,158 - ABIE53_000576 0.79 +0.7 627,158 - ABIE53_000576 0.79 -0.3 627,158 - ABIE53_000576 0.79 +0.4 627,166 + ABIE53_000576 0.80 +0.0 627,166 + ABIE53_000576 0.80 -0.9 627,167 - ABIE53_000576 0.80 -0.0 627,167 - ABIE53_000576 0.80 -2.1 627,167 - ABIE53_000576 0.80 +0.4 627,167 - ABIE53_000576 0.80 -0.7 627,370 + +0.4 627,370 + +0.6 627,370 + -1.1 627,370 + -3.3 627,370 + -0.1 627,371 - -1.2 627,371 - -0.3 627,371 - -0.3 627,371 - -0.8 627,371 - -1.8 627,371 - -0.5 627,380 + -0.6 627,380 + +1.4 627,381 - +1.0 627,522 - ABIE53_000577 0.13 -3.6 627,869 + ABIE53_000577 0.45 -3.5 627,869 + ABIE53_000577 0.45 -3.5 627,870 - ABIE53_000577 0.45 -4.1 627,912 + ABIE53_000577 0.49 -3.2 627,913 - ABIE53_000577 0.49 -2.1 627,913 - ABIE53_000577 0.49 -3.5 627,929 + ABIE53_000577 0.50 -0.4 627,929 + ABIE53_000577 0.50 -0.7 627,929 + ABIE53_000577 0.50 +1.0 627,929 + ABIE53_000577 0.50 -1.0 627,929 + ABIE53_000577 0.50 -0.3 627,930 - ABIE53_000577 0.50 -0.6 627,930 - ABIE53_000577 0.50 -2.1 627,930 - ABIE53_000577 0.50 -1.2 627,932 + ABIE53_000577 0.51 -1.8 627,932 + ABIE53_000577 0.51 -1.1 627,932 + ABIE53_000577 0.51 -2.7 627,932 + ABIE53_000577 0.51 -0.6 627,933 - ABIE53_000577 0.51 -4.1 628,203 - ABIE53_000577 0.75 -3.8 628,331 + ABIE53_000577 0.87 -1.1 628,332 - ABIE53_000577 0.87 -4.1
Or see this region's nucleotide sequence