Strain Fitness in Paraburkholderia graminis OAS925 around ABIE53_001544

Experiment: R2A with Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABIE53_001542 and ABIE53_001543 are separated by 4 nucleotidesABIE53_001543 and ABIE53_001544 are separated by 178 nucleotidesABIE53_001544 and ABIE53_001545 are separated by 194 nucleotides ABIE53_001542: ABIE53_001542 - ribosome-associated protein, at 1,719,483 to 1,719,938 _001542 ABIE53_001543: ABIE53_001543 - nicotinate-nucleotide adenylyltransferase, at 1,719,943 to 1,720,548 _001543 ABIE53_001544: ABIE53_001544 - coproporphyrinogen III oxidase, at 1,720,727 to 1,721,650 _001544 ABIE53_001545: ABIE53_001545 - phosphoribosylamine--glycine ligase, at 1,721,845 to 1,723,143 _001545 Position (kb) 1720 1721 1722Strain fitness (log2 ratio) -1 0 1at 1719.932 kb on + strandat 1719.932 kb on + strandat 1719.932 kb on + strandat 1719.932 kb on + strandat 1719.933 kb on - strandat 1720.662 kb on - strandat 1720.804 kb on - strandat 1721.122 kb on - strand, within ABIE53_001544at 1721.122 kb on - strand, within ABIE53_001544at 1721.229 kb on + strand, within ABIE53_001544at 1721.311 kb on + strand, within ABIE53_001544at 1721.312 kb on - strand, within ABIE53_001544at 1721.312 kb on - strand, within ABIE53_001544at 1721.312 kb on - strand, within ABIE53_001544at 1721.795 kb on + strandat 1721.795 kb on + strandat 1721.796 kb on - strandat 1721.796 kb on - strandat 1721.796 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Chloride 200 mM
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1,719,932 + -0.8
1,719,932 + -0.8
1,719,932 + -0.5
1,719,932 + -0.4
1,719,933 - -0.1
1,720,662 - -1.2
1,720,804 - +0.3
1,721,122 - ABIE53_001544 0.43 -0.7
1,721,122 - ABIE53_001544 0.43 -0.0
1,721,229 + ABIE53_001544 0.54 -0.3
1,721,311 + ABIE53_001544 0.63 -1.0
1,721,312 - ABIE53_001544 0.63 +1.3
1,721,312 - ABIE53_001544 0.63 -1.5
1,721,312 - ABIE53_001544 0.63 -1.2
1,721,795 + -1.1
1,721,795 + -1.2
1,721,796 - +0.8
1,721,796 - +0.3
1,721,796 - -1.0

Or see this region's nucleotide sequence