Experiment: M9 with Isopropyl alcohol 21 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_001226 and ABIE53_001227 are separated by 22 nucleotides ABIE53_001227 and ABIE53_001228 overlap by 4 nucleotides ABIE53_001228 and ABIE53_001229 are separated by 127 nucleotides
ABIE53_001226: ABIE53_001226 - sulfite reductase (NADPH) hemoprotein beta-component, at 1,369,843 to 1,371,522
_001226
ABIE53_001227: ABIE53_001227 - uncharacterized protein (DUF934 family), at 1,371,545 to 1,372,102
_001227
ABIE53_001228: ABIE53_001228 - phosphoadenosine phosphosulfate reductase, at 1,372,099 to 1,372,851
_001228
ABIE53_001229: ABIE53_001229 - sulfate adenylyltransferase subunit 2, at 1,372,979 to 1,373,941
_001229
Position (kb)
1372
1373 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1371.204 kb on + strand, within ABIE53_001226 at 1371.205 kb on - strand, within ABIE53_001226 at 1371.454 kb on + strand at 1371.454 kb on + strand at 1371.455 kb on - strand at 1371.455 kb on - strand at 1371.481 kb on - strand at 1371.481 kb on - strand at 1371.493 kb on + strand at 1371.520 kb on + strand at 1371.520 kb on + strand at 1371.535 kb on + strand at 1371.536 kb on - strand at 1371.553 kb on + strand at 1371.553 kb on + strand at 1371.553 kb on + strand at 1371.554 kb on - strand at 1371.554 kb on - strand at 1371.831 kb on - strand, within ABIE53_001227 at 1371.867 kb on - strand, within ABIE53_001227 at 1371.867 kb on - strand, within ABIE53_001227 at 1372.011 kb on - strand, within ABIE53_001227 at 1372.113 kb on - strand at 1372.346 kb on - strand, within ABIE53_001228 at 1372.397 kb on - strand, within ABIE53_001228 at 1372.397 kb on - strand, within ABIE53_001228 at 1372.397 kb on - strand, within ABIE53_001228 at 1372.397 kb on - strand, within ABIE53_001228 at 1372.427 kb on - strand, within ABIE53_001228 at 1372.427 kb on - strand, within ABIE53_001228 at 1372.427 kb on - strand, within ABIE53_001228 at 1372.427 kb on - strand, within ABIE53_001228 at 1372.640 kb on - strand, within ABIE53_001228 at 1372.861 kb on + strand at 1372.913 kb on + strand at 1373.074 kb on + strand at 1373.074 kb on + strand at 1373.222 kb on - strand, within ABIE53_001229 at 1373.240 kb on + strand, within ABIE53_001229 at 1373.240 kb on + strand, within ABIE53_001229 at 1373.241 kb on - strand, within ABIE53_001229 at 1373.241 kb on - strand, within ABIE53_001229 at 1373.414 kb on + strand, within ABIE53_001229 at 1373.414 kb on + strand, within ABIE53_001229 at 1373.414 kb on + strand, within ABIE53_001229 at 1373.414 kb on + strand, within ABIE53_001229 at 1373.415 kb on - strand, within ABIE53_001229 at 1373.415 kb on - strand, within ABIE53_001229 at 1373.417 kb on - strand, within ABIE53_001229 at 1373.543 kb on + strand, within ABIE53_001229 at 1373.543 kb on + strand, within ABIE53_001229 at 1373.544 kb on - strand, within ABIE53_001229 at 1373.621 kb on + strand, within ABIE53_001229 at 1373.622 kb on - strand, within ABIE53_001229 at 1373.657 kb on + strand, within ABIE53_001229 at 1373.657 kb on + strand, within ABIE53_001229 at 1373.657 kb on + strand, within ABIE53_001229 at 1373.657 kb on + strand, within ABIE53_001229 at 1373.657 kb on + strand, within ABIE53_001229 at 1373.658 kb on - strand, within ABIE53_001229 at 1373.658 kb on - strand, within ABIE53_001229 at 1373.658 kb on - strand, within ABIE53_001229 at 1373.785 kb on + strand, within ABIE53_001229 at 1373.785 kb on + strand, within ABIE53_001229 at 1373.785 kb on + strand, within ABIE53_001229 at 1373.785 kb on + strand, within ABIE53_001229 at 1373.786 kb on - strand, within ABIE53_001229
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Isopropyl alcohol 21 g/L remove 1,371,204 + ABIE53_001226 0.81 -4.3 1,371,205 - ABIE53_001226 0.81 -2.3 1,371,454 + -0.7 1,371,454 + -3.0 1,371,455 - -0.5 1,371,455 - -1.3 1,371,481 - -3.3 1,371,481 - -1.4 1,371,493 + -1.4 1,371,520 + +0.7 1,371,520 + +1.2 1,371,535 + -2.0 1,371,536 - -1.8 1,371,553 + -0.1 1,371,553 + -1.2 1,371,553 + -1.3 1,371,554 - -2.2 1,371,554 - -0.2 1,371,831 - ABIE53_001227 0.51 -2.4 1,371,867 - ABIE53_001227 0.58 +0.1 1,371,867 - ABIE53_001227 0.58 -0.7 1,372,011 - ABIE53_001227 0.84 -2.4 1,372,113 - -1.0 1,372,346 - ABIE53_001228 0.33 -0.6 1,372,397 - ABIE53_001228 0.40 -5.4 1,372,397 - ABIE53_001228 0.40 +2.3 1,372,397 - ABIE53_001228 0.40 -2.6 1,372,397 - ABIE53_001228 0.40 -5.1 1,372,427 - ABIE53_001228 0.44 -2.7 1,372,427 - ABIE53_001228 0.44 -2.8 1,372,427 - ABIE53_001228 0.44 -4.0 1,372,427 - ABIE53_001228 0.44 -1.2 1,372,640 - ABIE53_001228 0.72 -1.9 1,372,861 + +0.5 1,372,913 + -0.5 1,373,074 + -2.1 1,373,074 + -2.6 1,373,222 - ABIE53_001229 0.25 -1.5 1,373,240 + ABIE53_001229 0.27 -4.7 1,373,240 + ABIE53_001229 0.27 -0.8 1,373,241 - ABIE53_001229 0.27 -1.3 1,373,241 - ABIE53_001229 0.27 -3.5 1,373,414 + ABIE53_001229 0.45 -2.5 1,373,414 + ABIE53_001229 0.45 -1.7 1,373,414 + ABIE53_001229 0.45 -5.5 1,373,414 + ABIE53_001229 0.45 -3.0 1,373,415 - ABIE53_001229 0.45 +0.0 1,373,415 - ABIE53_001229 0.45 -0.9 1,373,417 - ABIE53_001229 0.45 -1.8 1,373,543 + ABIE53_001229 0.59 -2.5 1,373,543 + ABIE53_001229 0.59 +0.5 1,373,544 - ABIE53_001229 0.59 -0.1 1,373,621 + ABIE53_001229 0.67 -4.6 1,373,622 - ABIE53_001229 0.67 -5.2 1,373,657 + ABIE53_001229 0.70 -1.9 1,373,657 + ABIE53_001229 0.70 -0.4 1,373,657 + ABIE53_001229 0.70 -4.3 1,373,657 + ABIE53_001229 0.70 -2.3 1,373,657 + ABIE53_001229 0.70 -4.5 1,373,658 - ABIE53_001229 0.71 -2.0 1,373,658 - ABIE53_001229 0.71 -1.5 1,373,658 - ABIE53_001229 0.71 -2.1 1,373,785 + ABIE53_001229 0.84 -1.4 1,373,785 + ABIE53_001229 0.84 -5.5 1,373,785 + ABIE53_001229 0.84 -1.3 1,373,785 + ABIE53_001229 0.84 -1.7 1,373,786 - ABIE53_001229 0.84 +0.5
Or see this region's nucleotide sequence