Experiment: M9 with Isopropyl alcohol 21 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_001310 and ABIE53_001311 are separated by 179 nucleotides ABIE53_001311 and ABIE53_001312 are separated by 244 nucleotides
ABIE53_001310: ABIE53_001310 - protein TonB, at 1,464,189 to 1,464,725
_001310
ABIE53_001311: ABIE53_001311 - argininosuccinate lyase, at 1,464,905 to 1,466,311
_001311
ABIE53_001312: ABIE53_001312 - transcriptional regulator with XRE-family HTH domain, at 1,466,556 to 1,467,368
_001312
Position (kb)
1464
1465
1466
1467 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1463.954 kb on + strand at 1463.954 kb on + strand at 1463.954 kb on + strand at 1463.955 kb on - strand at 1463.955 kb on - strand at 1463.955 kb on - strand at 1463.955 kb on - strand at 1463.955 kb on - strand at 1464.065 kb on + strand at 1464.065 kb on + strand at 1464.066 kb on - strand at 1464.066 kb on - strand at 1464.066 kb on - strand at 1464.133 kb on + strand at 1464.133 kb on + strand at 1464.385 kb on + strand, within ABIE53_001310 at 1464.385 kb on + strand, within ABIE53_001310 at 1464.385 kb on + strand, within ABIE53_001310 at 1464.386 kb on - strand, within ABIE53_001310 at 1464.386 kb on - strand, within ABIE53_001310 at 1464.481 kb on + strand, within ABIE53_001310 at 1464.481 kb on + strand, within ABIE53_001310 at 1464.481 kb on + strand, within ABIE53_001310 at 1464.481 kb on + strand, within ABIE53_001310 at 1464.481 kb on + strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.482 kb on - strand, within ABIE53_001310 at 1464.763 kb on + strand at 1464.763 kb on + strand at 1464.764 kb on - strand at 1464.825 kb on - strand at 1464.906 kb on + strand at 1464.907 kb on - strand at 1466.326 kb on - strand at 1466.326 kb on - strand at 1466.326 kb on - strand at 1466.326 kb on - strand at 1466.326 kb on - strand at 1466.360 kb on - strand at 1466.360 kb on - strand at 1466.360 kb on - strand at 1466.360 kb on - strand at 1466.455 kb on + strand at 1466.456 kb on - strand at 1466.624 kb on - strand at 1466.866 kb on + strand, within ABIE53_001312 at 1466.866 kb on + strand, within ABIE53_001312 at 1466.866 kb on + strand, within ABIE53_001312 at 1466.866 kb on + strand, within ABIE53_001312 at 1466.867 kb on - strand, within ABIE53_001312 at 1466.968 kb on + strand, within ABIE53_001312 at 1466.968 kb on + strand, within ABIE53_001312 at 1466.968 kb on + strand, within ABIE53_001312 at 1466.969 kb on - strand, within ABIE53_001312 at 1466.969 kb on - strand, within ABIE53_001312 at 1466.969 kb on - strand, within ABIE53_001312 at 1467.076 kb on + strand, within ABIE53_001312 at 1467.076 kb on + strand, within ABIE53_001312 at 1467.076 kb on + strand, within ABIE53_001312 at 1467.076 kb on + strand, within ABIE53_001312 at 1467.077 kb on - strand, within ABIE53_001312 at 1467.077 kb on - strand, within ABIE53_001312 at 1467.172 kb on + strand, within ABIE53_001312 at 1467.172 kb on + strand, within ABIE53_001312 at 1467.172 kb on + strand, within ABIE53_001312 at 1467.172 kb on + strand, within ABIE53_001312 at 1467.173 kb on - strand, within ABIE53_001312 at 1467.173 kb on - strand, within ABIE53_001312 at 1467.173 kb on - strand, within ABIE53_001312 at 1467.173 kb on - strand, within ABIE53_001312 at 1467.297 kb on - strand at 1467.297 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Isopropyl alcohol 21 g/L remove 1,463,954 + -0.4 1,463,954 + -1.0 1,463,954 + -0.1 1,463,955 - -0.8 1,463,955 - -0.2 1,463,955 - -0.1 1,463,955 - +2.2 1,463,955 - -0.2 1,464,065 + +0.1 1,464,065 + +0.3 1,464,066 - -0.1 1,464,066 - -0.1 1,464,066 - -0.4 1,464,133 + +0.5 1,464,133 + -0.1 1,464,385 + ABIE53_001310 0.36 -0.4 1,464,385 + ABIE53_001310 0.36 +0.8 1,464,385 + ABIE53_001310 0.36 -0.2 1,464,386 - ABIE53_001310 0.37 +0.5 1,464,386 - ABIE53_001310 0.37 +0.8 1,464,481 + ABIE53_001310 0.54 -0.5 1,464,481 + ABIE53_001310 0.54 -0.6 1,464,481 + ABIE53_001310 0.54 +0.4 1,464,481 + ABIE53_001310 0.54 +1.0 1,464,481 + ABIE53_001310 0.54 -0.8 1,464,482 - ABIE53_001310 0.55 -0.5 1,464,482 - ABIE53_001310 0.55 -0.2 1,464,482 - ABIE53_001310 0.55 -0.5 1,464,482 - ABIE53_001310 0.55 -0.8 1,464,482 - ABIE53_001310 0.55 -0.8 1,464,482 - ABIE53_001310 0.55 +0.0 1,464,482 - ABIE53_001310 0.55 -0.7 1,464,482 - ABIE53_001310 0.55 -1.0 1,464,482 - ABIE53_001310 0.55 -0.2 1,464,763 + -0.1 1,464,763 + +0.6 1,464,764 - -1.0 1,464,825 - -0.1 1,464,906 + -0.5 1,464,907 - -1.2 1,466,326 - +0.3 1,466,326 - -0.7 1,466,326 - +0.3 1,466,326 - -0.7 1,466,326 - +0.2 1,466,360 - -0.1 1,466,360 - -0.8 1,466,360 - +0.9 1,466,360 - -0.3 1,466,455 + -1.0 1,466,456 - +0.7 1,466,624 - +0.8 1,466,866 + ABIE53_001312 0.38 -1.3 1,466,866 + ABIE53_001312 0.38 -0.1 1,466,866 + ABIE53_001312 0.38 -0.3 1,466,866 + ABIE53_001312 0.38 -2.5 1,466,867 - ABIE53_001312 0.38 -1.7 1,466,968 + ABIE53_001312 0.51 -0.4 1,466,968 + ABIE53_001312 0.51 -0.0 1,466,968 + ABIE53_001312 0.51 -3.3 1,466,969 - ABIE53_001312 0.51 -0.0 1,466,969 - ABIE53_001312 0.51 +0.1 1,466,969 - ABIE53_001312 0.51 -0.8 1,467,076 + ABIE53_001312 0.64 -1.4 1,467,076 + ABIE53_001312 0.64 -2.4 1,467,076 + ABIE53_001312 0.64 -1.4 1,467,076 + ABIE53_001312 0.64 -0.9 1,467,077 - ABIE53_001312 0.64 -1.1 1,467,077 - ABIE53_001312 0.64 -2.1 1,467,172 + ABIE53_001312 0.76 -1.0 1,467,172 + ABIE53_001312 0.76 -0.2 1,467,172 + ABIE53_001312 0.76 -1.1 1,467,172 + ABIE53_001312 0.76 -2.4 1,467,173 - ABIE53_001312 0.76 -0.2 1,467,173 - ABIE53_001312 0.76 -1.4 1,467,173 - ABIE53_001312 0.76 -1.1 1,467,173 - ABIE53_001312 0.76 -1.2 1,467,297 - -0.3 1,467,297 - -2.6
Or see this region's nucleotide sequence