Experiment: R2A with Isopropyl alcohol 21 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_002050 and ABIE53_002051 are separated by 88 nucleotides ABIE53_002051 and ABIE53_002052 are separated by 78 nucleotides ABIE53_002052 and ABIE53_002053 are separated by 94 nucleotides
ABIE53_002050: ABIE53_002050 - NitT/TauT family transport system substrate-binding protein, at 2,299,109 to 2,300,146
_002050
ABIE53_002051: ABIE53_002051 - NitT/TauT family transport system ATP-binding protein, at 2,300,235 to 2,301,047
_002051
ABIE53_002052: ABIE53_002052 - NitT/TauT family transport system permease protein, at 2,301,126 to 2,301,950
_002052
ABIE53_002053: ABIE53_002053 - NADPH2:quinone reductase, at 2,302,045 to 2,303,019
_002053
Position (kb)
2301
2302 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2300.144 kb on + strand at 2300.145 kb on - strand at 2300.145 kb on - strand at 2300.145 kb on - strand at 2300.145 kb on - strand at 2300.198 kb on + strand at 2300.198 kb on + strand at 2300.199 kb on - strand at 2300.199 kb on - strand at 2300.199 kb on - strand at 2300.199 kb on - strand at 2300.199 kb on - strand at 2300.199 kb on - strand at 2300.307 kb on + strand at 2300.307 kb on + strand at 2300.307 kb on + strand at 2300.307 kb on + strand at 2300.309 kb on + strand at 2300.309 kb on + strand at 2300.310 kb on - strand at 2300.310 kb on - strand at 2300.310 kb on - strand at 2300.446 kb on + strand, within ABIE53_002051 at 2300.446 kb on + strand, within ABIE53_002051 at 2300.447 kb on - strand, within ABIE53_002051 at 2300.487 kb on + strand, within ABIE53_002051 at 2300.487 kb on + strand, within ABIE53_002051 at 2300.487 kb on + strand, within ABIE53_002051 at 2300.488 kb on - strand, within ABIE53_002051 at 2300.488 kb on - strand, within ABIE53_002051 at 2300.488 kb on - strand, within ABIE53_002051 at 2300.488 kb on - strand, within ABIE53_002051 at 2300.488 kb on - strand, within ABIE53_002051 at 2300.488 kb on - strand, within ABIE53_002051 at 2300.488 kb on - strand, within ABIE53_002051 at 2300.488 kb on - strand, within ABIE53_002051 at 2300.782 kb on + strand, within ABIE53_002051 at 2300.782 kb on + strand, within ABIE53_002051 at 2300.782 kb on + strand, within ABIE53_002051 at 2300.782 kb on + strand, within ABIE53_002051 at 2300.782 kb on + strand, within ABIE53_002051 at 2300.783 kb on - strand, within ABIE53_002051 at 2300.783 kb on - strand, within ABIE53_002051 at 2300.783 kb on - strand, within ABIE53_002051 at 2300.783 kb on - strand, within ABIE53_002051 at 2300.783 kb on - strand, within ABIE53_002051 at 2301.013 kb on + strand at 2301.013 kb on + strand at 2301.013 kb on + strand at 2301.013 kb on + strand at 2301.013 kb on + strand at 2301.014 kb on - strand at 2301.014 kb on - strand at 2301.014 kb on - strand at 2301.014 kb on - strand at 2301.021 kb on + strand at 2301.022 kb on - strand at 2301.043 kb on + strand at 2301.043 kb on + strand at 2301.043 kb on + strand at 2301.043 kb on + strand at 2301.043 kb on + strand at 2301.043 kb on + strand at 2301.043 kb on + strand at 2301.044 kb on - strand at 2301.044 kb on - strand at 2301.044 kb on - strand at 2301.044 kb on - strand at 2301.044 kb on - strand at 2301.083 kb on + strand at 2301.083 kb on + strand at 2301.083 kb on + strand at 2301.083 kb on + strand at 2301.084 kb on - strand at 2301.084 kb on - strand at 2301.084 kb on - strand at 2301.089 kb on + strand at 2301.089 kb on + strand at 2301.089 kb on + strand at 2301.089 kb on + strand at 2301.089 kb on + strand at 2301.090 kb on - strand at 2301.207 kb on + strand at 2301.207 kb on + strand at 2301.207 kb on + strand at 2301.208 kb on - strand at 2301.244 kb on - strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.336 kb on + strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.337 kb on - strand, within ABIE53_002052 at 2301.416 kb on - strand, within ABIE53_002052 at 2301.416 kb on - strand, within ABIE53_002052 at 2301.416 kb on - strand, within ABIE53_002052 at 2301.416 kb on - strand, within ABIE53_002052 at 2301.416 kb on - strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.459 kb on + strand, within ABIE53_002052 at 2301.460 kb on - strand, within ABIE53_002052 at 2301.460 kb on - strand, within ABIE53_002052 at 2301.460 kb on - strand, within ABIE53_002052 at 2301.460 kb on - strand, within ABIE53_002052 at 2301.460 kb on - strand, within ABIE53_002052 at 2301.460 kb on - strand, within ABIE53_002052 at 2301.460 kb on - strand, within ABIE53_002052 at 2301.591 kb on + strand, within ABIE53_002052 at 2301.591 kb on + strand, within ABIE53_002052 at 2301.591 kb on + strand, within ABIE53_002052 at 2301.591 kb on + strand, within ABIE53_002052 at 2301.592 kb on - strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.672 kb on + strand, within ABIE53_002052 at 2301.673 kb on - strand, within ABIE53_002052 at 2301.673 kb on - strand, within ABIE53_002052 at 2301.673 kb on - strand, within ABIE53_002052 at 2301.673 kb on - strand, within ABIE53_002052 at 2301.673 kb on - strand, within ABIE53_002052 at 2301.673 kb on - strand, within ABIE53_002052 at 2301.678 kb on + strand, within ABIE53_002052 at 2301.678 kb on + strand, within ABIE53_002052 at 2301.678 kb on + strand, within ABIE53_002052 at 2301.678 kb on + strand, within ABIE53_002052 at 2301.678 kb on + strand, within ABIE53_002052 at 2301.679 kb on - strand, within ABIE53_002052 at 2301.679 kb on - strand, within ABIE53_002052 at 2301.762 kb on + strand, within ABIE53_002052 at 2301.762 kb on + strand, within ABIE53_002052 at 2301.762 kb on + strand, within ABIE53_002052 at 2301.762 kb on + strand, within ABIE53_002052 at 2301.789 kb on + strand, within ABIE53_002052 at 2301.790 kb on - strand, within ABIE53_002052 at 2301.949 kb on - strand at 2301.949 kb on - strand at 2302.046 kb on + strand at 2302.089 kb on + strand at 2302.090 kb on - strand at 2302.319 kb on + strand, within ABIE53_002053 at 2302.319 kb on + strand, within ABIE53_002053 at 2302.361 kb on + strand, within ABIE53_002053 at 2302.361 kb on + strand, within ABIE53_002053 at 2302.362 kb on - strand, within ABIE53_002053 at 2302.362 kb on - strand, within ABIE53_002053 at 2302.362 kb on - strand, within ABIE53_002053 at 2302.362 kb on - strand, within ABIE53_002053 at 2302.362 kb on - strand, within ABIE53_002053 at 2302.382 kb on + strand, within ABIE53_002053 at 2302.382 kb on + strand, within ABIE53_002053 at 2302.382 kb on + strand, within ABIE53_002053 at 2302.382 kb on + strand, within ABIE53_002053 at 2302.382 kb on + strand, within ABIE53_002053 at 2302.382 kb on + strand, within ABIE53_002053 at 2302.382 kb on + strand, within ABIE53_002053 at 2302.383 kb on - strand, within ABIE53_002053 at 2302.383 kb on - strand, within ABIE53_002053 at 2302.383 kb on - strand, within ABIE53_002053 at 2302.383 kb on - strand, within ABIE53_002053 at 2302.448 kb on + strand, within ABIE53_002053 at 2302.449 kb on - strand, within ABIE53_002053 at 2302.449 kb on - strand, within ABIE53_002053 at 2302.449 kb on - strand, within ABIE53_002053 at 2302.449 kb on - strand, within ABIE53_002053 at 2302.449 kb on - strand, within ABIE53_002053 at 2302.649 kb on + strand, within ABIE53_002053 at 2302.650 kb on - strand, within ABIE53_002053 at 2302.843 kb on - strand, within ABIE53_002053 at 2302.843 kb on - strand, within ABIE53_002053 at 2302.843 kb on - strand, within ABIE53_002053 at 2302.868 kb on + strand, within ABIE53_002053 at 2302.868 kb on + strand, within ABIE53_002053 at 2302.868 kb on + strand, within ABIE53_002053 at 2302.868 kb on + strand, within ABIE53_002053 at 2302.868 kb on + strand, within ABIE53_002053 at 2302.868 kb on + strand, within ABIE53_002053 at 2302.868 kb on + strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053 at 2302.869 kb on - strand, within ABIE53_002053
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Isopropyl alcohol 21 g/L remove 2,300,144 + -0.7 2,300,145 - -0.4 2,300,145 - +0.6 2,300,145 - +0.9 2,300,145 - -0.4 2,300,198 + -0.7 2,300,198 + +0.6 2,300,199 - -0.0 2,300,199 - -0.0 2,300,199 - -0.0 2,300,199 - -0.8 2,300,199 - -0.3 2,300,199 - -1.0 2,300,307 + -1.0 2,300,307 + -0.8 2,300,307 + +1.6 2,300,307 + +0.6 2,300,309 + -0.5 2,300,309 + -0.7 2,300,310 - -0.6 2,300,310 - -0.6 2,300,310 - -0.2 2,300,446 + ABIE53_002051 0.26 -0.7 2,300,446 + ABIE53_002051 0.26 -0.5 2,300,447 - ABIE53_002051 0.26 -0.4 2,300,487 + ABIE53_002051 0.31 -2.3 2,300,487 + ABIE53_002051 0.31 -2.4 2,300,487 + ABIE53_002051 0.31 -0.3 2,300,488 - ABIE53_002051 0.31 -2.8 2,300,488 - ABIE53_002051 0.31 +0.6 2,300,488 - ABIE53_002051 0.31 +0.4 2,300,488 - ABIE53_002051 0.31 -0.4 2,300,488 - ABIE53_002051 0.31 -0.5 2,300,488 - ABIE53_002051 0.31 +1.4 2,300,488 - ABIE53_002051 0.31 -0.9 2,300,488 - ABIE53_002051 0.31 -0.4 2,300,782 + ABIE53_002051 0.67 -0.4 2,300,782 + ABIE53_002051 0.67 -1.2 2,300,782 + ABIE53_002051 0.67 +0.7 2,300,782 + ABIE53_002051 0.67 -1.4 2,300,782 + ABIE53_002051 0.67 +0.4 2,300,783 - ABIE53_002051 0.67 -0.0 2,300,783 - ABIE53_002051 0.67 -0.4 2,300,783 - ABIE53_002051 0.67 +0.7 2,300,783 - ABIE53_002051 0.67 -0.9 2,300,783 - ABIE53_002051 0.67 -0.7 2,301,013 + -1.4 2,301,013 + +0.7 2,301,013 + -0.8 2,301,013 + -0.8 2,301,013 + +0.2 2,301,014 - -0.1 2,301,014 - +1.0 2,301,014 - -2.6 2,301,014 - +0.3 2,301,021 + -0.4 2,301,022 - -0.0 2,301,043 + -0.6 2,301,043 + -0.6 2,301,043 + +0.6 2,301,043 + +0.5 2,301,043 + -1.3 2,301,043 + -0.8 2,301,043 + +0.5 2,301,044 - -0.8 2,301,044 - +0.4 2,301,044 - +0.2 2,301,044 - -0.5 2,301,044 - -1.1 2,301,083 + +0.8 2,301,083 + -1.4 2,301,083 + -0.8 2,301,083 + -2.2 2,301,084 - -0.1 2,301,084 - +0.2 2,301,084 - -0.3 2,301,089 + +0.4 2,301,089 + -0.5 2,301,089 + -0.8 2,301,089 + -1.4 2,301,089 + +1.2 2,301,090 - +0.2 2,301,207 + +1.2 2,301,207 + -0.8 2,301,207 + -0.8 2,301,208 - +0.5 2,301,244 - ABIE53_002052 0.14 -0.8 2,301,336 + ABIE53_002052 0.25 -0.8 2,301,336 + ABIE53_002052 0.25 -1.0 2,301,336 + ABIE53_002052 0.25 +0.5 2,301,336 + ABIE53_002052 0.25 +1.7 2,301,336 + ABIE53_002052 0.25 -1.3 2,301,336 + ABIE53_002052 0.25 -0.5 2,301,336 + ABIE53_002052 0.25 -0.3 2,301,336 + ABIE53_002052 0.25 -0.5 2,301,336 + ABIE53_002052 0.25 -0.4 2,301,336 + ABIE53_002052 0.25 +0.0 2,301,336 + ABIE53_002052 0.25 +0.4 2,301,336 + ABIE53_002052 0.25 -1.6 2,301,336 + ABIE53_002052 0.25 +2.2 2,301,336 + ABIE53_002052 0.25 -0.5 2,301,336 + ABIE53_002052 0.25 -1.5 2,301,336 + ABIE53_002052 0.25 +1.9 2,301,337 - ABIE53_002052 0.26 -0.7 2,301,337 - ABIE53_002052 0.26 -1.0 2,301,337 - ABIE53_002052 0.26 -0.2 2,301,337 - ABIE53_002052 0.26 +1.3 2,301,337 - ABIE53_002052 0.26 -0.5 2,301,337 - ABIE53_002052 0.26 -0.8 2,301,337 - ABIE53_002052 0.26 +0.0 2,301,337 - ABIE53_002052 0.26 -0.4 2,301,337 - ABIE53_002052 0.26 -0.6 2,301,337 - ABIE53_002052 0.26 -1.2 2,301,416 - ABIE53_002052 0.35 -0.2 2,301,416 - ABIE53_002052 0.35 -0.3 2,301,416 - ABIE53_002052 0.35 -0.5 2,301,416 - ABIE53_002052 0.35 -0.9 2,301,416 - ABIE53_002052 0.35 -1.3 2,301,459 + ABIE53_002052 0.40 -0.1 2,301,459 + ABIE53_002052 0.40 -0.9 2,301,459 + ABIE53_002052 0.40 -0.6 2,301,459 + ABIE53_002052 0.40 +0.1 2,301,459 + ABIE53_002052 0.40 -0.5 2,301,459 + ABIE53_002052 0.40 -0.4 2,301,459 + ABIE53_002052 0.40 -0.8 2,301,459 + ABIE53_002052 0.40 +0.0 2,301,459 + ABIE53_002052 0.40 -0.4 2,301,460 - ABIE53_002052 0.40 +0.4 2,301,460 - ABIE53_002052 0.40 -0.8 2,301,460 - ABIE53_002052 0.40 -0.6 2,301,460 - ABIE53_002052 0.40 -0.5 2,301,460 - ABIE53_002052 0.40 -0.2 2,301,460 - ABIE53_002052 0.40 -0.3 2,301,460 - ABIE53_002052 0.40 -1.8 2,301,591 + ABIE53_002052 0.56 -0.5 2,301,591 + ABIE53_002052 0.56 -0.4 2,301,591 + ABIE53_002052 0.56 +1.2 2,301,591 + ABIE53_002052 0.56 -0.6 2,301,592 - ABIE53_002052 0.56 -0.0 2,301,672 + ABIE53_002052 0.66 -0.3 2,301,672 + ABIE53_002052 0.66 -0.2 2,301,672 + ABIE53_002052 0.66 -0.7 2,301,672 + ABIE53_002052 0.66 -0.2 2,301,672 + ABIE53_002052 0.66 +0.3 2,301,672 + ABIE53_002052 0.66 -0.7 2,301,672 + ABIE53_002052 0.66 +0.2 2,301,672 + ABIE53_002052 0.66 -0.6 2,301,672 + ABIE53_002052 0.66 -0.8 2,301,672 + ABIE53_002052 0.66 -0.3 2,301,672 + ABIE53_002052 0.66 -0.7 2,301,672 + ABIE53_002052 0.66 -1.2 2,301,673 - ABIE53_002052 0.66 -0.1 2,301,673 - ABIE53_002052 0.66 -0.4 2,301,673 - ABIE53_002052 0.66 -0.3 2,301,673 - ABIE53_002052 0.66 -1.2 2,301,673 - ABIE53_002052 0.66 -0.9 2,301,673 - ABIE53_002052 0.66 +0.8 2,301,678 + ABIE53_002052 0.67 -0.9 2,301,678 + ABIE53_002052 0.67 -0.4 2,301,678 + ABIE53_002052 0.67 -0.8 2,301,678 + ABIE53_002052 0.67 +0.2 2,301,678 + ABIE53_002052 0.67 -0.7 2,301,679 - ABIE53_002052 0.67 -0.2 2,301,679 - ABIE53_002052 0.67 -0.4 2,301,762 + ABIE53_002052 0.77 -1.0 2,301,762 + ABIE53_002052 0.77 -0.4 2,301,762 + ABIE53_002052 0.77 +0.2 2,301,762 + ABIE53_002052 0.77 +0.8 2,301,789 + ABIE53_002052 0.80 -0.1 2,301,790 - ABIE53_002052 0.80 +0.2 2,301,949 - -1.2 2,301,949 - +0.1 2,302,046 + -1.2 2,302,089 + -1.1 2,302,090 - +0.7 2,302,319 + ABIE53_002053 0.28 -0.4 2,302,319 + ABIE53_002053 0.28 +1.0 2,302,361 + ABIE53_002053 0.32 -1.2 2,302,361 + ABIE53_002053 0.32 -0.4 2,302,362 - ABIE53_002053 0.33 -0.6 2,302,362 - ABIE53_002053 0.33 +0.6 2,302,362 - ABIE53_002053 0.33 -1.0 2,302,362 - ABIE53_002053 0.33 +0.1 2,302,362 - ABIE53_002053 0.33 +0.3 2,302,382 + ABIE53_002053 0.35 -0.6 2,302,382 + ABIE53_002053 0.35 -0.4 2,302,382 + ABIE53_002053 0.35 -0.8 2,302,382 + ABIE53_002053 0.35 -1.0 2,302,382 + ABIE53_002053 0.35 -0.2 2,302,382 + ABIE53_002053 0.35 -0.6 2,302,382 + ABIE53_002053 0.35 -0.7 2,302,383 - ABIE53_002053 0.35 -0.2 2,302,383 - ABIE53_002053 0.35 +0.4 2,302,383 - ABIE53_002053 0.35 -0.0 2,302,383 - ABIE53_002053 0.35 -0.2 2,302,448 + ABIE53_002053 0.41 -1.1 2,302,449 - ABIE53_002053 0.41 -1.6 2,302,449 - ABIE53_002053 0.41 -1.0 2,302,449 - ABIE53_002053 0.41 +0.4 2,302,449 - ABIE53_002053 0.41 -0.2 2,302,449 - ABIE53_002053 0.41 +1.2 2,302,649 + ABIE53_002053 0.62 -0.9 2,302,650 - ABIE53_002053 0.62 +0.7 2,302,843 - ABIE53_002053 0.82 -0.5 2,302,843 - ABIE53_002053 0.82 -0.4 2,302,843 - ABIE53_002053 0.82 -0.5 2,302,868 + ABIE53_002053 0.84 -0.5 2,302,868 + ABIE53_002053 0.84 -1.1 2,302,868 + ABIE53_002053 0.84 +0.8 2,302,868 + ABIE53_002053 0.84 +0.6 2,302,868 + ABIE53_002053 0.84 +0.3 2,302,868 + ABIE53_002053 0.84 +1.4 2,302,868 + ABIE53_002053 0.84 +0.6 2,302,869 - ABIE53_002053 0.85 +0.4 2,302,869 - ABIE53_002053 0.85 -0.6 2,302,869 - ABIE53_002053 0.85 +0.1 2,302,869 - ABIE53_002053 0.85 +0.4 2,302,869 - ABIE53_002053 0.85 +0.8 2,302,869 - ABIE53_002053 0.85 +1.6 2,302,869 - ABIE53_002053 0.85 -0.7 2,302,869 - ABIE53_002053 0.85 -0.7 2,302,869 - ABIE53_002053 0.85 -0.6 2,302,869 - ABIE53_002053 0.85 +0.4 2,302,869 - ABIE53_002053 0.85 -0.4 2,302,869 - ABIE53_002053 0.85 +0.6 2,302,869 - ABIE53_002053 0.85 -0.9
Or see this region's nucleotide sequence