Experiment: M9 with Ethanol 2.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000426 and ABIE53_000427 are separated by 72 nucleotides ABIE53_000427 and ABIE53_000428 are separated by 57 nucleotides ABIE53_000428 and ABIE53_000429 overlap by 4 nucleotides
ABIE53_000426: ABIE53_000426 - integrase/recombinase XerC, at 461,641 to 462,564
_000426
ABIE53_000427: ABIE53_000427 - uncharacterized protein YigA (DUF484 family), at 462,637 to 463,392
_000427
ABIE53_000428: ABIE53_000428 - diaminopimelate epimerase, at 463,450 to 464,352
_000428
ABIE53_000429: ABIE53_000429 - KDO2-lipid IV(A) lauroyltransferase, at 464,349 to 465,233
_000429
Position (kb)
462
463
464 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 461.678 kb on + strand at 461.714 kb on + strand at 461.714 kb on + strand at 461.714 kb on + strand at 461.715 kb on - strand at 462.326 kb on + strand, within ABIE53_000426 at 462.327 kb on - strand, within ABIE53_000426 at 462.411 kb on + strand, within ABIE53_000426 at 462.536 kb on + strand at 462.536 kb on + strand at 462.536 kb on + strand at 462.537 kb on - strand at 462.537 kb on - strand at 462.537 kb on - strand at 462.927 kb on - strand, within ABIE53_000427 at 462.998 kb on + strand, within ABIE53_000427 at 462.999 kb on - strand, within ABIE53_000427 at 462.999 kb on - strand, within ABIE53_000427 at 463.089 kb on - strand, within ABIE53_000427 at 463.332 kb on - strand at 463.332 kb on - strand at 463.332 kb on - strand at 463.375 kb on - strand at 463.375 kb on - strand at 463.375 kb on - strand at 463.414 kb on + strand at 463.414 kb on + strand at 463.414 kb on + strand at 463.415 kb on - strand at 463.415 kb on - strand at 463.415 kb on - strand at 463.415 kb on - strand at 463.415 kb on - strand at 463.415 kb on - strand at 464.319 kb on + strand at 464.320 kb on - strand at 464.320 kb on - strand at 464.320 kb on - strand at 464.320 kb on - strand at 464.320 kb on - strand at 464.320 kb on - strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.324 kb on + strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.325 kb on - strand at 464.353 kb on + strand at 464.353 kb on + strand at 464.353 kb on + strand at 464.354 kb on - strand at 464.354 kb on - strand at 464.354 kb on - strand at 464.354 kb on - strand at 464.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Ethanol 2.5 vol% remove 461,678 + +1.7 461,714 + -0.2 461,714 + -0.1 461,714 + -1.4 461,715 - -0.6 462,326 + ABIE53_000426 0.74 +0.7 462,327 - ABIE53_000426 0.74 +0.1 462,411 + ABIE53_000426 0.83 +2.1 462,536 + +1.7 462,536 + +0.0 462,536 + +1.0 462,537 - -0.8 462,537 - +0.2 462,537 - -0.5 462,927 - ABIE53_000427 0.38 +0.1 462,998 + ABIE53_000427 0.48 -0.6 462,999 - ABIE53_000427 0.48 -1.6 462,999 - ABIE53_000427 0.48 -2.1 463,089 - ABIE53_000427 0.60 -2.3 463,332 - -0.7 463,332 - -2.5 463,332 - -1.0 463,375 - -0.5 463,375 - +1.1 463,375 - -0.2 463,414 + +1.0 463,414 + -0.6 463,414 + -0.6 463,415 - -1.3 463,415 - -0.6 463,415 - -0.3 463,415 - -0.5 463,415 - +0.1 463,415 - +0.0 464,319 + -1.0 464,320 - -0.9 464,320 - -0.6 464,320 - -0.4 464,320 - -1.1 464,320 - -1.1 464,320 - +0.4 464,324 + +0.7 464,324 + -0.5 464,324 + +1.0 464,324 + -0.4 464,324 + -0.5 464,324 + -0.2 464,324 + -0.6 464,324 + -0.1 464,324 + -0.3 464,324 + -0.9 464,325 - -0.5 464,325 - -0.4 464,325 - +0.2 464,325 - +0.8 464,325 - +0.3 464,325 - -0.5 464,325 - +0.8 464,325 - -1.1 464,325 - +1.8 464,325 - +0.8 464,325 - +0.0 464,353 + -0.5 464,353 + -3.8 464,353 + -0.8 464,354 - -2.7 464,354 - -4.2 464,354 - -1.5 464,354 - -1.7 464,354 - -0.8
Or see this region's nucleotide sequence