Experiment: M9 with 3-methyl-2-butenol 2 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_002808 and ABIE53_002809 are separated by 30 nucleotides ABIE53_002809 and ABIE53_002810 are separated by 183 nucleotides
ABIE53_002808: ABIE53_002808 - molybdopterin-biosynthesis enzyme MoeA-like protein, at 3,166,708 to 3,167,535
_002808
ABIE53_002809: ABIE53_002809 - hypothetical protein, at 3,167,566 to 3,168,399
_002809
ABIE53_002810: ABIE53_002810 - sterol desaturase/sphingolipid hydroxylase (fatty acid hydroxylase superfamily), at 3,168,583 to 3,169,482
_002810
Position (kb)
3167
3168
3169 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3166.650 kb on + strand at 3166.651 kb on - strand at 3166.654 kb on - strand at 3166.746 kb on + strand at 3166.777 kb on + strand at 3166.777 kb on + strand at 3166.778 kb on - strand at 3166.847 kb on + strand, within ABIE53_002808 at 3166.847 kb on + strand, within ABIE53_002808 at 3166.847 kb on + strand, within ABIE53_002808 at 3166.847 kb on + strand, within ABIE53_002808 at 3166.847 kb on + strand, within ABIE53_002808 at 3166.847 kb on + strand, within ABIE53_002808 at 3166.848 kb on - strand, within ABIE53_002808 at 3166.848 kb on - strand, within ABIE53_002808 at 3166.848 kb on - strand, within ABIE53_002808 at 3166.940 kb on - strand, within ABIE53_002808 at 3167.012 kb on + strand, within ABIE53_002808 at 3167.013 kb on - strand, within ABIE53_002808 at 3167.075 kb on + strand, within ABIE53_002808 at 3167.075 kb on + strand, within ABIE53_002808 at 3167.075 kb on + strand, within ABIE53_002808 at 3167.076 kb on - strand, within ABIE53_002808 at 3167.076 kb on - strand, within ABIE53_002808 at 3167.076 kb on - strand, within ABIE53_002808 at 3167.076 kb on - strand, within ABIE53_002808 at 3167.076 kb on - strand, within ABIE53_002808 at 3167.111 kb on + strand, within ABIE53_002808 at 3167.111 kb on + strand, within ABIE53_002808 at 3167.111 kb on + strand, within ABIE53_002808 at 3167.112 kb on - strand, within ABIE53_002808 at 3167.144 kb on + strand, within ABIE53_002808 at 3167.144 kb on + strand, within ABIE53_002808 at 3167.144 kb on + strand, within ABIE53_002808 at 3167.144 kb on + strand, within ABIE53_002808 at 3167.144 kb on + strand, within ABIE53_002808 at 3167.144 kb on + strand, within ABIE53_002808 at 3167.144 kb on + strand, within ABIE53_002808 at 3167.145 kb on - strand, within ABIE53_002808 at 3167.145 kb on - strand, within ABIE53_002808 at 3167.145 kb on - strand, within ABIE53_002808 at 3167.145 kb on - strand, within ABIE53_002808 at 3167.154 kb on + strand, within ABIE53_002808 at 3167.155 kb on - strand, within ABIE53_002808 at 3167.216 kb on + strand, within ABIE53_002808 at 3167.216 kb on + strand, within ABIE53_002808 at 3167.216 kb on + strand, within ABIE53_002808 at 3167.217 kb on - strand, within ABIE53_002808 at 3167.217 kb on - strand, within ABIE53_002808 at 3167.370 kb on + strand, within ABIE53_002808 at 3167.414 kb on + strand, within ABIE53_002808 at 3167.414 kb on + strand, within ABIE53_002808 at 3167.414 kb on + strand, within ABIE53_002808 at 3167.414 kb on + strand, within ABIE53_002808 at 3167.415 kb on - strand, within ABIE53_002808 at 3167.415 kb on - strand, within ABIE53_002808 at 3167.415 kb on - strand, within ABIE53_002808 at 3167.551 kb on + strand at 3167.551 kb on + strand at 3167.551 kb on + strand at 3167.552 kb on - strand at 3167.570 kb on + strand at 3167.570 kb on + strand at 3167.571 kb on - strand at 3167.571 kb on - strand at 3167.571 kb on - strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.642 kb on + strand at 3167.643 kb on - strand at 3167.643 kb on - strand at 3167.643 kb on - strand at 3167.643 kb on - strand at 3167.643 kb on - strand at 3167.643 kb on - strand at 3167.646 kb on - strand at 3167.646 kb on - strand at 3167.646 kb on - strand at 3167.684 kb on + strand, within ABIE53_002809 at 3167.684 kb on + strand, within ABIE53_002809 at 3167.685 kb on - strand, within ABIE53_002809 at 3167.685 kb on - strand, within ABIE53_002809 at 3167.685 kb on - strand, within ABIE53_002809 at 3167.870 kb on + strand, within ABIE53_002809 at 3167.870 kb on + strand, within ABIE53_002809 at 3167.870 kb on + strand, within ABIE53_002809 at 3167.870 kb on + strand, within ABIE53_002809 at 3167.870 kb on + strand, within ABIE53_002809 at 3167.870 kb on + strand, within ABIE53_002809 at 3167.870 kb on + strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.871 kb on - strand, within ABIE53_002809 at 3167.885 kb on + strand, within ABIE53_002809 at 3167.885 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.977 kb on + strand, within ABIE53_002809 at 3167.978 kb on - strand, within ABIE53_002809 at 3167.978 kb on - strand, within ABIE53_002809 at 3167.978 kb on - strand, within ABIE53_002809 at 3167.978 kb on - strand, within ABIE53_002809 at 3167.978 kb on - strand, within ABIE53_002809 at 3167.978 kb on - strand, within ABIE53_002809 at 3167.978 kb on - strand, within ABIE53_002809 at 3168.014 kb on + strand, within ABIE53_002809 at 3168.112 kb on + strand, within ABIE53_002809 at 3168.113 kb on - strand, within ABIE53_002809 at 3168.113 kb on - strand, within ABIE53_002809 at 3168.113 kb on - strand, within ABIE53_002809 at 3168.113 kb on - strand, within ABIE53_002809 at 3168.113 kb on - strand, within ABIE53_002809 at 3168.209 kb on + strand, within ABIE53_002809 at 3168.209 kb on + strand, within ABIE53_002809 at 3168.209 kb on + strand, within ABIE53_002809 at 3168.210 kb on - strand, within ABIE53_002809 at 3168.210 kb on - strand, within ABIE53_002809 at 3168.276 kb on + strand, within ABIE53_002809 at 3168.276 kb on + strand, within ABIE53_002809 at 3168.277 kb on - strand, within ABIE53_002809 at 3168.277 kb on - strand, within ABIE53_002809 at 3168.277 kb on - strand, within ABIE53_002809 at 3168.277 kb on - strand, within ABIE53_002809 at 3168.461 kb on + strand at 3168.462 kb on - strand at 3168.462 kb on - strand at 3168.489 kb on + strand at 3168.489 kb on + strand at 3168.489 kb on + strand at 3168.489 kb on + strand at 3168.490 kb on - strand at 3168.490 kb on - strand at 3168.804 kb on - strand, within ABIE53_002810 at 3168.804 kb on - strand, within ABIE53_002810 at 3168.903 kb on + strand, within ABIE53_002810 at 3169.127 kb on + strand, within ABIE53_002810 at 3169.127 kb on + strand, within ABIE53_002810 at 3169.127 kb on + strand, within ABIE53_002810 at 3169.128 kb on - strand, within ABIE53_002810 at 3169.128 kb on - strand, within ABIE53_002810 at 3169.128 kb on - strand, within ABIE53_002810 at 3169.160 kb on + strand, within ABIE53_002810 at 3169.160 kb on + strand, within ABIE53_002810 at 3169.160 kb on + strand, within ABIE53_002810 at 3169.160 kb on + strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.161 kb on - strand, within ABIE53_002810 at 3169.233 kb on + strand, within ABIE53_002810 at 3169.233 kb on + strand, within ABIE53_002810 at 3169.233 kb on + strand, within ABIE53_002810 at 3169.233 kb on + strand, within ABIE53_002810 at 3169.233 kb on + strand, within ABIE53_002810 at 3169.233 kb on + strand, within ABIE53_002810 at 3169.233 kb on + strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.234 kb on - strand, within ABIE53_002810 at 3169.328 kb on + strand, within ABIE53_002810 at 3169.328 kb on + strand, within ABIE53_002810 at 3169.329 kb on - strand, within ABIE53_002810 at 3169.329 kb on - strand, within ABIE53_002810 at 3169.329 kb on - strand, within ABIE53_002810
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with 3-methyl-2-butenol 2 g/L remove 3,166,650 + -0.7 3,166,651 - -0.8 3,166,654 - +0.3 3,166,746 + +1.1 3,166,777 + +1.9 3,166,777 + -0.3 3,166,778 - -0.5 3,166,847 + ABIE53_002808 0.17 +1.0 3,166,847 + ABIE53_002808 0.17 +1.8 3,166,847 + ABIE53_002808 0.17 +0.4 3,166,847 + ABIE53_002808 0.17 +0.3 3,166,847 + ABIE53_002808 0.17 -0.8 3,166,847 + ABIE53_002808 0.17 +0.2 3,166,848 - ABIE53_002808 0.17 +0.0 3,166,848 - ABIE53_002808 0.17 -0.0 3,166,848 - ABIE53_002808 0.17 +0.3 3,166,940 - ABIE53_002808 0.28 -1.0 3,167,012 + ABIE53_002808 0.37 -0.1 3,167,013 - ABIE53_002808 0.37 +0.1 3,167,075 + ABIE53_002808 0.44 +0.5 3,167,075 + ABIE53_002808 0.44 -0.0 3,167,075 + ABIE53_002808 0.44 -0.1 3,167,076 - ABIE53_002808 0.44 -0.5 3,167,076 - ABIE53_002808 0.44 +0.6 3,167,076 - ABIE53_002808 0.44 +0.3 3,167,076 - ABIE53_002808 0.44 +0.4 3,167,076 - ABIE53_002808 0.44 +0.4 3,167,111 + ABIE53_002808 0.49 -1.3 3,167,111 + ABIE53_002808 0.49 +0.1 3,167,111 + ABIE53_002808 0.49 +0.2 3,167,112 - ABIE53_002808 0.49 -1.6 3,167,144 + ABIE53_002808 0.53 +0.1 3,167,144 + ABIE53_002808 0.53 +0.2 3,167,144 + ABIE53_002808 0.53 -0.5 3,167,144 + ABIE53_002808 0.53 +0.1 3,167,144 + ABIE53_002808 0.53 -0.2 3,167,144 + ABIE53_002808 0.53 -1.3 3,167,144 + ABIE53_002808 0.53 -1.1 3,167,145 - ABIE53_002808 0.53 +2.0 3,167,145 - ABIE53_002808 0.53 -0.5 3,167,145 - ABIE53_002808 0.53 +1.2 3,167,145 - ABIE53_002808 0.53 -1.0 3,167,154 + ABIE53_002808 0.54 +0.1 3,167,155 - ABIE53_002808 0.54 +0.3 3,167,216 + ABIE53_002808 0.61 +1.1 3,167,216 + ABIE53_002808 0.61 -0.5 3,167,216 + ABIE53_002808 0.61 -0.2 3,167,217 - ABIE53_002808 0.61 -1.4 3,167,217 - ABIE53_002808 0.61 -0.8 3,167,370 + ABIE53_002808 0.80 +0.7 3,167,414 + ABIE53_002808 0.85 +0.1 3,167,414 + ABIE53_002808 0.85 -0.4 3,167,414 + ABIE53_002808 0.85 -0.5 3,167,414 + ABIE53_002808 0.85 +0.8 3,167,415 - ABIE53_002808 0.85 -0.0 3,167,415 - ABIE53_002808 0.85 -0.3 3,167,415 - ABIE53_002808 0.85 -0.4 3,167,551 + -0.6 3,167,551 + -0.1 3,167,551 + +1.7 3,167,552 - -0.8 3,167,570 + +0.7 3,167,570 + +0.1 3,167,571 - -1.3 3,167,571 - -0.4 3,167,571 - +0.6 3,167,642 + -0.2 3,167,642 + +1.2 3,167,642 + +0.6 3,167,642 + +0.2 3,167,642 + +0.3 3,167,642 + +0.3 3,167,642 + +0.2 3,167,642 + +0.2 3,167,642 + -0.2 3,167,642 + -0.4 3,167,642 + -0.8 3,167,642 + -0.4 3,167,643 - +0.1 3,167,643 - -0.3 3,167,643 - -0.1 3,167,643 - +1.0 3,167,643 - -0.4 3,167,643 - +1.4 3,167,646 - -0.2 3,167,646 - -0.1 3,167,646 - -1.1 3,167,684 + ABIE53_002809 0.14 -0.3 3,167,684 + ABIE53_002809 0.14 -1.1 3,167,685 - ABIE53_002809 0.14 +0.3 3,167,685 - ABIE53_002809 0.14 +0.4 3,167,685 - ABIE53_002809 0.14 -0.4 3,167,870 + ABIE53_002809 0.36 -0.7 3,167,870 + ABIE53_002809 0.36 -1.0 3,167,870 + ABIE53_002809 0.36 -0.1 3,167,870 + ABIE53_002809 0.36 +0.3 3,167,870 + ABIE53_002809 0.36 +0.6 3,167,870 + ABIE53_002809 0.36 -0.7 3,167,870 + ABIE53_002809 0.36 -0.5 3,167,871 - ABIE53_002809 0.37 -0.9 3,167,871 - ABIE53_002809 0.37 +0.6 3,167,871 - ABIE53_002809 0.37 -0.1 3,167,871 - ABIE53_002809 0.37 +0.3 3,167,871 - ABIE53_002809 0.37 +1.2 3,167,871 - ABIE53_002809 0.37 -1.1 3,167,871 - ABIE53_002809 0.37 -0.5 3,167,871 - ABIE53_002809 0.37 -0.5 3,167,871 - ABIE53_002809 0.37 -0.7 3,167,871 - ABIE53_002809 0.37 -1.1 3,167,885 + ABIE53_002809 0.38 -1.8 3,167,885 + ABIE53_002809 0.38 +0.7 3,167,977 + ABIE53_002809 0.49 +0.9 3,167,977 + ABIE53_002809 0.49 -0.6 3,167,977 + ABIE53_002809 0.49 -0.3 3,167,977 + ABIE53_002809 0.49 +0.6 3,167,977 + ABIE53_002809 0.49 -0.9 3,167,977 + ABIE53_002809 0.49 -0.2 3,167,977 + ABIE53_002809 0.49 -0.1 3,167,977 + ABIE53_002809 0.49 -0.1 3,167,977 + ABIE53_002809 0.49 -1.0 3,167,978 - ABIE53_002809 0.49 -2.8 3,167,978 - ABIE53_002809 0.49 -1.0 3,167,978 - ABIE53_002809 0.49 -0.7 3,167,978 - ABIE53_002809 0.49 -0.6 3,167,978 - ABIE53_002809 0.49 -0.1 3,167,978 - ABIE53_002809 0.49 +0.3 3,167,978 - ABIE53_002809 0.49 +0.2 3,168,014 + ABIE53_002809 0.54 +0.8 3,168,112 + ABIE53_002809 0.65 +0.9 3,168,113 - ABIE53_002809 0.66 +1.1 3,168,113 - ABIE53_002809 0.66 -0.8 3,168,113 - ABIE53_002809 0.66 -1.5 3,168,113 - ABIE53_002809 0.66 -0.8 3,168,113 - ABIE53_002809 0.66 -1.0 3,168,209 + ABIE53_002809 0.77 -0.8 3,168,209 + ABIE53_002809 0.77 +0.4 3,168,209 + ABIE53_002809 0.77 +0.8 3,168,210 - ABIE53_002809 0.77 -0.9 3,168,210 - ABIE53_002809 0.77 -0.7 3,168,276 + ABIE53_002809 0.85 -0.5 3,168,276 + ABIE53_002809 0.85 -2.3 3,168,277 - ABIE53_002809 0.85 -0.1 3,168,277 - ABIE53_002809 0.85 +0.4 3,168,277 - ABIE53_002809 0.85 -0.7 3,168,277 - ABIE53_002809 0.85 -0.3 3,168,461 + +1.2 3,168,462 - +0.2 3,168,462 - +1.2 3,168,489 + -1.0 3,168,489 + +0.5 3,168,489 + +0.3 3,168,489 + +1.0 3,168,490 - +0.4 3,168,490 - +1.7 3,168,804 - ABIE53_002810 0.25 +1.5 3,168,804 - ABIE53_002810 0.25 +1.5 3,168,903 + ABIE53_002810 0.36 +2.3 3,169,127 + ABIE53_002810 0.60 +1.7 3,169,127 + ABIE53_002810 0.60 +3.0 3,169,127 + ABIE53_002810 0.60 +0.1 3,169,128 - ABIE53_002810 0.61 +1.4 3,169,128 - ABIE53_002810 0.61 +0.1 3,169,128 - ABIE53_002810 0.61 +0.6 3,169,160 + ABIE53_002810 0.64 +0.8 3,169,160 + ABIE53_002810 0.64 -0.2 3,169,160 + ABIE53_002810 0.64 +2.8 3,169,160 + ABIE53_002810 0.64 +2.8 3,169,161 - ABIE53_002810 0.64 +2.0 3,169,161 - ABIE53_002810 0.64 -0.2 3,169,161 - ABIE53_002810 0.64 +1.3 3,169,161 - ABIE53_002810 0.64 +1.1 3,169,161 - ABIE53_002810 0.64 +0.8 3,169,161 - ABIE53_002810 0.64 +0.6 3,169,161 - ABIE53_002810 0.64 +1.1 3,169,161 - ABIE53_002810 0.64 +0.6 3,169,161 - ABIE53_002810 0.64 +2.0 3,169,161 - ABIE53_002810 0.64 +0.9 3,169,233 + ABIE53_002810 0.72 +1.4 3,169,233 + ABIE53_002810 0.72 +2.8 3,169,233 + ABIE53_002810 0.72 +2.0 3,169,233 + ABIE53_002810 0.72 +2.2 3,169,233 + ABIE53_002810 0.72 +1.3 3,169,233 + ABIE53_002810 0.72 +1.2 3,169,233 + ABIE53_002810 0.72 +1.1 3,169,234 - ABIE53_002810 0.72 +1.2 3,169,234 - ABIE53_002810 0.72 +1.8 3,169,234 - ABIE53_002810 0.72 +2.4 3,169,234 - ABIE53_002810 0.72 +0.9 3,169,234 - ABIE53_002810 0.72 +0.9 3,169,234 - ABIE53_002810 0.72 +0.8 3,169,234 - ABIE53_002810 0.72 +0.4 3,169,234 - ABIE53_002810 0.72 +0.4 3,169,234 - ABIE53_002810 0.72 +1.3 3,169,234 - ABIE53_002810 0.72 +0.4 3,169,234 - ABIE53_002810 0.72 +0.3 3,169,234 - ABIE53_002810 0.72 +0.5 3,169,234 - ABIE53_002810 0.72 +0.6 3,169,234 - ABIE53_002810 0.72 +0.1 3,169,234 - ABIE53_002810 0.72 +1.0 3,169,234 - ABIE53_002810 0.72 +0.5 3,169,234 - ABIE53_002810 0.72 +0.6 3,169,234 - ABIE53_002810 0.72 +1.0 3,169,328 + ABIE53_002810 0.83 +2.7 3,169,328 + ABIE53_002810 0.83 +1.7 3,169,329 - ABIE53_002810 0.83 +2.3 3,169,329 - ABIE53_002810 0.83 +1.1 3,169,329 - ABIE53_002810 0.83 -0.2
Or see this region's nucleotide sequence