Experiment: M9 with 3-methyl-3-butenol 4.5 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_004465 and ABIE53_004466 are separated by 462 nucleotides ABIE53_004466 and ABIE53_004467 are separated by 5 nucleotides ABIE53_004467 and ABIE53_004468 are separated by 19 nucleotides
ABIE53_004465: ABIE53_004465 - hypothetical protein, at 655,523 to 656,017
_004465
ABIE53_004466: ABIE53_004466 - hypothetical protein, at 656,480 to 656,767
_004466
ABIE53_004467: ABIE53_004467 - protein phosphatase, at 656,773 to 657,543
_004467
ABIE53_004468: ABIE53_004468 - transcriptional regulator with GAF, ATPase, and Fis domain, at 657,563 to 658,921
_004468
Position (kb) 656
657
658 Strain fitness (log2 ratio) -2
-1
0
1
2 at 655.896 kb on + strand, within ABIE53_004465 at 655.896 kb on + strand, within ABIE53_004465 at 655.896 kb on + strand, within ABIE53_004465 at 655.896 kb on + strand, within ABIE53_004465 at 655.897 kb on - strand, within ABIE53_004465 at 655.897 kb on - strand, within ABIE53_004465 at 655.897 kb on - strand, within ABIE53_004465 at 655.897 kb on - strand, within ABIE53_004465 at 656.125 kb on + strand at 656.304 kb on + strand at 656.305 kb on - strand at 656.371 kb on + strand at 656.420 kb on + strand at 656.420 kb on + strand at 656.421 kb on - strand at 656.488 kb on + strand at 656.488 kb on + strand at 656.488 kb on + strand at 656.489 kb on - strand at 656.594 kb on + strand, within ABIE53_004466 at 656.594 kb on + strand, within ABIE53_004466 at 656.594 kb on + strand, within ABIE53_004466 at 656.594 kb on + strand, within ABIE53_004466 at 656.595 kb on - strand, within ABIE53_004466 at 656.657 kb on + strand, within ABIE53_004466 at 656.657 kb on + strand, within ABIE53_004466 at 656.657 kb on + strand, within ABIE53_004466 at 656.657 kb on + strand, within ABIE53_004466 at 656.657 kb on + strand, within ABIE53_004466 at 656.657 kb on + strand, within ABIE53_004466 at 656.657 kb on + strand, within ABIE53_004466 at 656.657 kb on + strand, within ABIE53_004466 at 656.658 kb on - strand, within ABIE53_004466 at 656.759 kb on - strand at 656.759 kb on - strand at 657.116 kb on - strand, within ABIE53_004467 at 657.116 kb on - strand, within ABIE53_004467 at 657.116 kb on - strand, within ABIE53_004467 at 657.143 kb on - strand, within ABIE53_004467 at 657.222 kb on + strand, within ABIE53_004467 at 657.258 kb on + strand, within ABIE53_004467 at 657.258 kb on + strand, within ABIE53_004467 at 657.258 kb on + strand, within ABIE53_004467 at 657.259 kb on - strand, within ABIE53_004467 at 657.264 kb on + strand, within ABIE53_004467 at 657.265 kb on - strand, within ABIE53_004467 at 657.265 kb on - strand, within ABIE53_004467 at 657.431 kb on + strand, within ABIE53_004467 at 657.432 kb on - strand, within ABIE53_004467 at 657.505 kb on + strand at 657.506 kb on - strand at 657.993 kb on + strand, within ABIE53_004468 at 657.994 kb on - strand, within ABIE53_004468 at 657.994 kb on - strand, within ABIE53_004468 at 657.994 kb on - strand, within ABIE53_004468 at 657.994 kb on - strand, within ABIE53_004468 at 658.149 kb on + strand, within ABIE53_004468 at 658.340 kb on - strand, within ABIE53_004468 at 658.433 kb on + strand, within ABIE53_004468 at 658.434 kb on - strand, within ABIE53_004468 at 658.468 kb on + strand, within ABIE53_004468 at 658.477 kb on + strand, within ABIE53_004468
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with 3-methyl-3-butenol 4.5 g/L remove 655,896 + ABIE53_004465 0.75 +0.6 655,896 + ABIE53_004465 0.75 +0.1 655,896 + ABIE53_004465 0.75 +0.1 655,896 + ABIE53_004465 0.75 -1.1 655,897 - ABIE53_004465 0.76 -0.3 655,897 - ABIE53_004465 0.76 -0.5 655,897 - ABIE53_004465 0.76 -0.4 655,897 - ABIE53_004465 0.76 +0.3 656,125 + -0.1 656,304 + -0.4 656,305 - +0.1 656,371 + +0.7 656,420 + +1.2 656,420 + -0.3 656,421 - -1.7 656,488 + +0.0 656,488 + +0.8 656,488 + +0.5 656,489 - -0.1 656,594 + ABIE53_004466 0.40 -0.2 656,594 + ABIE53_004466 0.40 -0.3 656,594 + ABIE53_004466 0.40 -1.0 656,594 + ABIE53_004466 0.40 +1.0 656,595 - ABIE53_004466 0.40 +0.3 656,657 + ABIE53_004466 0.61 -0.0 656,657 + ABIE53_004466 0.61 -1.8 656,657 + ABIE53_004466 0.61 -1.1 656,657 + ABIE53_004466 0.61 +0.3 656,657 + ABIE53_004466 0.61 +0.2 656,657 + ABIE53_004466 0.61 -1.3 656,657 + ABIE53_004466 0.61 -0.3 656,657 + ABIE53_004466 0.61 -0.9 656,658 - ABIE53_004466 0.62 -0.5 656,759 - -0.1 656,759 - +0.2 657,116 - ABIE53_004467 0.44 -0.3 657,116 - ABIE53_004467 0.44 -0.9 657,116 - ABIE53_004467 0.44 -0.0 657,143 - ABIE53_004467 0.48 +0.4 657,222 + ABIE53_004467 0.58 -1.2 657,258 + ABIE53_004467 0.63 -0.9 657,258 + ABIE53_004467 0.63 -0.9 657,258 + ABIE53_004467 0.63 -2.0 657,259 - ABIE53_004467 0.63 -0.6 657,264 + ABIE53_004467 0.64 +0.5 657,265 - ABIE53_004467 0.64 -1.0 657,265 - ABIE53_004467 0.64 -0.3 657,431 + ABIE53_004467 0.85 +2.4 657,432 - ABIE53_004467 0.85 -0.5 657,505 + -1.5 657,506 - +1.3 657,993 + ABIE53_004468 0.32 +0.4 657,994 - ABIE53_004468 0.32 +0.2 657,994 - ABIE53_004468 0.32 -1.6 657,994 - ABIE53_004468 0.32 -0.5 657,994 - ABIE53_004468 0.32 -1.2 658,149 + ABIE53_004468 0.43 +0.1 658,340 - ABIE53_004468 0.57 -1.4 658,433 + ABIE53_004468 0.64 +1.0 658,434 - ABIE53_004468 0.64 -0.5 658,468 + ABIE53_004468 0.67 -1.5 658,477 + ABIE53_004468 0.67 +0.3
Or see this region's nucleotide sequence