Experiment: R2A with 3-methyl-3-butenol 4.5 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_006396 and ABIE53_006397 are separated by 97 nucleotides ABIE53_006397 and ABIE53_006398 are separated by 36 nucleotides ABIE53_006398 and ABIE53_006399 are separated by 130 nucleotides
ABIE53_006396: ABIE53_006396 - DNA-binding HxlR family transcriptional regulator, at 2,774,276 to 2,774,650
_006396
ABIE53_006397: ABIE53_006397 - putative dinucleotide-binding enzyme, at 2,774,748 to 2,775,509
_006397
ABIE53_006398: ABIE53_006398 - FMN-dependent oxidoreductase (nitrilotriacetate monooxygenase family), at 2,775,546 to 2,776,901
_006398
ABIE53_006399: ABIE53_006399 - peptide/nickel transport system substrate-binding protein, at 2,777,032 to 2,778,621
_006399
Position (kb)
2775
2776
2777 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2774.667 kb on + strand at 2774.667 kb on + strand at 2774.679 kb on - strand at 2774.689 kb on + strand at 2774.690 kb on - strand at 2774.690 kb on - strand at 2774.690 kb on - strand at 2774.696 kb on - strand at 2774.696 kb on - strand at 2774.741 kb on + strand at 2774.742 kb on - strand at 2774.956 kb on + strand, within ABIE53_006397 at 2775.024 kb on + strand, within ABIE53_006397 at 2775.024 kb on + strand, within ABIE53_006397 at 2775.025 kb on - strand, within ABIE53_006397 at 2775.145 kb on - strand, within ABIE53_006397 at 2775.325 kb on - strand, within ABIE53_006397 at 2775.516 kb on + strand at 2775.517 kb on - strand at 2775.539 kb on + strand at 2775.539 kb on + strand at 2775.552 kb on + strand at 2775.552 kb on + strand at 2775.625 kb on + strand at 2775.666 kb on + strand at 2775.715 kb on - strand, within ABIE53_006398 at 2775.736 kb on + strand, within ABIE53_006398 at 2775.736 kb on + strand, within ABIE53_006398 at 2775.736 kb on + strand, within ABIE53_006398 at 2775.737 kb on - strand, within ABIE53_006398 at 2776.669 kb on + strand, within ABIE53_006398 at 2776.784 kb on - strand at 2777.043 kb on + strand at 2777.046 kb on - strand at 2777.046 kb on - strand at 2777.046 kb on - strand at 2777.091 kb on - strand at 2777.091 kb on - strand at 2777.121 kb on + strand at 2777.155 kb on - strand at 2777.245 kb on + strand, within ABIE53_006399 at 2777.391 kb on - strand, within ABIE53_006399 at 2777.391 kb on - strand, within ABIE53_006399 at 2777.405 kb on + strand, within ABIE53_006399 at 2777.405 kb on + strand, within ABIE53_006399 at 2777.405 kb on + strand, within ABIE53_006399 at 2777.405 kb on + strand, within ABIE53_006399 at 2777.406 kb on - strand, within ABIE53_006399 at 2777.406 kb on - strand, within ABIE53_006399 at 2777.505 kb on - strand, within ABIE53_006399 at 2777.505 kb on - strand, within ABIE53_006399 at 2777.547 kb on + strand, within ABIE53_006399 at 2777.548 kb on - strand, within ABIE53_006399 at 2777.549 kb on + strand, within ABIE53_006399 at 2777.600 kb on + strand, within ABIE53_006399 at 2777.601 kb on - strand, within ABIE53_006399 at 2777.621 kb on + strand, within ABIE53_006399
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-methyl-3-butenol 4.5 g/L remove 2,774,667 + -0.4 2,774,667 + -0.6 2,774,679 - +0.0 2,774,689 + -1.4 2,774,690 - +0.9 2,774,690 - +0.4 2,774,690 - -0.3 2,774,696 - +0.3 2,774,696 - +0.1 2,774,741 + +0.3 2,774,742 - -0.2 2,774,956 + ABIE53_006397 0.27 -1.1 2,775,024 + ABIE53_006397 0.36 +0.3 2,775,024 + ABIE53_006397 0.36 +0.1 2,775,025 - ABIE53_006397 0.36 +0.5 2,775,145 - ABIE53_006397 0.52 -1.0 2,775,325 - ABIE53_006397 0.76 -1.0 2,775,516 + +0.9 2,775,517 - +1.1 2,775,539 + -0.9 2,775,539 + -2.3 2,775,552 + +1.0 2,775,552 + +0.8 2,775,625 + +0.1 2,775,666 + +0.1 2,775,715 - ABIE53_006398 0.12 +1.7 2,775,736 + ABIE53_006398 0.14 -0.8 2,775,736 + ABIE53_006398 0.14 -0.5 2,775,736 + ABIE53_006398 0.14 +1.1 2,775,737 - ABIE53_006398 0.14 -0.2 2,776,669 + ABIE53_006398 0.83 -3.1 2,776,784 - -0.8 2,777,043 + +0.1 2,777,046 - -0.2 2,777,046 - +0.1 2,777,046 - +0.6 2,777,091 - -0.7 2,777,091 - -0.2 2,777,121 + +0.2 2,777,155 - -0.0 2,777,245 + ABIE53_006399 0.13 +0.6 2,777,391 - ABIE53_006399 0.23 +0.5 2,777,391 - ABIE53_006399 0.23 -1.9 2,777,405 + ABIE53_006399 0.23 -0.3 2,777,405 + ABIE53_006399 0.23 +2.2 2,777,405 + ABIE53_006399 0.23 -0.7 2,777,405 + ABIE53_006399 0.23 +0.9 2,777,406 - ABIE53_006399 0.24 +0.2 2,777,406 - ABIE53_006399 0.24 -1.5 2,777,505 - ABIE53_006399 0.30 +0.3 2,777,505 - ABIE53_006399 0.30 -2.3 2,777,547 + ABIE53_006399 0.32 +0.5 2,777,548 - ABIE53_006399 0.32 -0.1 2,777,549 + ABIE53_006399 0.33 -0.3 2,777,600 + ABIE53_006399 0.36 -1.0 2,777,601 - ABIE53_006399 0.36 +0.9 2,777,621 + ABIE53_006399 0.37 +0.1
Or see this region's nucleotide sequence