Experiment: R2A with 3-methyl-3-butenol 4.5 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_005847 and ABIE53_005848 are separated by 19 nucleotides ABIE53_005848 and ABIE53_005849 are separated by 5 nucleotides ABIE53_005849 and ABIE53_005850 are separated by 27 nucleotides ABIE53_005850 and ABIE53_005851 are separated by 62 nucleotides ABIE53_005851 and ABIE53_005852 are separated by 55 nucleotides
ABIE53_005847: ABIE53_005847 - L-lactate dehydrogenase (cytochrome), at 2,158,546 to 2,159,724
_005847
ABIE53_005848: ABIE53_005848 - NAD(P)-dependent dehydrogenase (short-subunit alcohol dehydrogenase family), at 2,159,744 to 2,160,538
_005848
ABIE53_005849: ABIE53_005849 - uncharacterized protein YndB with AHSA1/START domain, at 2,160,544 to 2,161,047
_005849
ABIE53_005850: ABIE53_005850 - hypothetical protein, at 2,161,075 to 2,161,569
_005850
ABIE53_005851: ABIE53_005851 - hypothetical protein, at 2,161,632 to 2,161,808
_005851
ABIE53_005852: ABIE53_005852 - DNA-binding transcriptional LysR family regulator, at 2,161,864 to 2,162,745
_005852
Position (kb)
2160
2161
2162 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2159.609 kb on + strand at 2159.610 kb on - strand at 2159.627 kb on + strand at 2159.628 kb on - strand at 2159.628 kb on - strand at 2159.628 kb on - strand at 2159.722 kb on + strand at 2159.723 kb on - strand at 2159.729 kb on + strand at 2159.729 kb on + strand at 2159.730 kb on - strand at 2159.823 kb on + strand at 2159.823 kb on + strand at 2159.824 kb on - strand, within ABIE53_005848 at 2159.824 kb on - strand, within ABIE53_005848 at 2159.852 kb on - strand, within ABIE53_005848 at 2160.016 kb on + strand, within ABIE53_005848 at 2160.078 kb on + strand, within ABIE53_005848 at 2160.079 kb on - strand, within ABIE53_005848 at 2160.101 kb on - strand, within ABIE53_005848 at 2160.168 kb on + strand, within ABIE53_005848 at 2160.168 kb on + strand, within ABIE53_005848 at 2160.168 kb on + strand, within ABIE53_005848 at 2160.169 kb on - strand, within ABIE53_005848 at 2160.169 kb on - strand, within ABIE53_005848 at 2160.169 kb on - strand, within ABIE53_005848 at 2160.430 kb on + strand, within ABIE53_005848 at 2160.431 kb on - strand, within ABIE53_005848 at 2160.538 kb on + strand at 2160.539 kb on - strand at 2160.545 kb on + strand at 2160.545 kb on + strand at 2160.546 kb on - strand at 2160.562 kb on + strand at 2160.562 kb on + strand at 2160.563 kb on - strand at 2160.563 kb on - strand at 2160.611 kb on + strand, within ABIE53_005849 at 2160.617 kb on + strand, within ABIE53_005849 at 2160.617 kb on + strand, within ABIE53_005849 at 2160.617 kb on + strand, within ABIE53_005849 at 2160.618 kb on - strand, within ABIE53_005849 at 2160.618 kb on - strand, within ABIE53_005849 at 2160.845 kb on + strand, within ABIE53_005849 at 2160.846 kb on - strand, within ABIE53_005849 at 2160.846 kb on - strand, within ABIE53_005849 at 2160.846 kb on - strand, within ABIE53_005849 at 2160.912 kb on - strand, within ABIE53_005849 at 2160.992 kb on + strand, within ABIE53_005849 at 2161.016 kb on + strand at 2161.016 kb on + strand at 2161.049 kb on + strand at 2161.050 kb on - strand at 2161.256 kb on + strand, within ABIE53_005850 at 2161.257 kb on - strand, within ABIE53_005850 at 2161.366 kb on + strand, within ABIE53_005850 at 2161.375 kb on - strand, within ABIE53_005850 at 2161.375 kb on - strand, within ABIE53_005850 at 2161.375 kb on - strand, within ABIE53_005850 at 2161.430 kb on + strand, within ABIE53_005850 at 2161.431 kb on - strand, within ABIE53_005850 at 2161.565 kb on + strand at 2161.565 kb on + strand at 2161.566 kb on - strand at 2161.566 kb on - strand at 2161.566 kb on - strand at 2161.566 kb on - strand at 2161.566 kb on - strand at 2161.566 kb on - strand at 2161.692 kb on + strand, within ABIE53_005851 at 2161.693 kb on - strand, within ABIE53_005851 at 2161.751 kb on + strand, within ABIE53_005851 at 2161.752 kb on - strand, within ABIE53_005851 at 2161.752 kb on - strand, within ABIE53_005851 at 2161.752 kb on - strand, within ABIE53_005851 at 2161.765 kb on + strand, within ABIE53_005851 at 2161.765 kb on + strand, within ABIE53_005851 at 2161.766 kb on - strand, within ABIE53_005851 at 2161.809 kb on - strand at 2161.966 kb on - strand, within ABIE53_005852
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-methyl-3-butenol 4.5 g/L remove 2,159,609 + -1.6 2,159,610 - -0.9 2,159,627 + +0.8 2,159,628 - +0.5 2,159,628 - -0.5 2,159,628 - -1.0 2,159,722 + +1.4 2,159,723 - +0.8 2,159,729 + +0.3 2,159,729 + +0.3 2,159,730 - -0.4 2,159,823 + +0.2 2,159,823 + -0.6 2,159,824 - ABIE53_005848 0.10 +0.7 2,159,824 - ABIE53_005848 0.10 -0.2 2,159,852 - ABIE53_005848 0.14 +0.7 2,160,016 + ABIE53_005848 0.34 +0.5 2,160,078 + ABIE53_005848 0.42 -0.4 2,160,079 - ABIE53_005848 0.42 -0.2 2,160,101 - ABIE53_005848 0.45 -0.7 2,160,168 + ABIE53_005848 0.53 +0.5 2,160,168 + ABIE53_005848 0.53 -0.2 2,160,168 + ABIE53_005848 0.53 -0.3 2,160,169 - ABIE53_005848 0.53 +0.1 2,160,169 - ABIE53_005848 0.53 +1.3 2,160,169 - ABIE53_005848 0.53 -0.3 2,160,430 + ABIE53_005848 0.86 +0.3 2,160,431 - ABIE53_005848 0.86 -0.5 2,160,538 + -1.3 2,160,539 - -0.2 2,160,545 + -0.2 2,160,545 + -2.0 2,160,546 - -0.1 2,160,562 + -0.6 2,160,562 + +2.1 2,160,563 - +0.9 2,160,563 - -0.2 2,160,611 + ABIE53_005849 0.13 -0.2 2,160,617 + ABIE53_005849 0.14 -0.6 2,160,617 + ABIE53_005849 0.14 -0.9 2,160,617 + ABIE53_005849 0.14 +2.5 2,160,618 - ABIE53_005849 0.15 -0.5 2,160,618 - ABIE53_005849 0.15 -1.4 2,160,845 + ABIE53_005849 0.60 -0.3 2,160,846 - ABIE53_005849 0.60 -0.4 2,160,846 - ABIE53_005849 0.60 -0.7 2,160,846 - ABIE53_005849 0.60 +0.1 2,160,912 - ABIE53_005849 0.73 +0.4 2,160,992 + ABIE53_005849 0.89 +1.9 2,161,016 + +1.1 2,161,016 + -1.0 2,161,049 + -0.4 2,161,050 - +0.9 2,161,256 + ABIE53_005850 0.37 -0.9 2,161,257 - ABIE53_005850 0.37 +0.0 2,161,366 + ABIE53_005850 0.59 -1.3 2,161,375 - ABIE53_005850 0.61 +0.3 2,161,375 - ABIE53_005850 0.61 +0.0 2,161,375 - ABIE53_005850 0.61 -0.3 2,161,430 + ABIE53_005850 0.72 -0.5 2,161,431 - ABIE53_005850 0.72 -1.2 2,161,565 + +1.4 2,161,565 + +0.3 2,161,566 - -1.0 2,161,566 - -0.6 2,161,566 - -0.8 2,161,566 - -0.1 2,161,566 - -0.1 2,161,566 - +1.1 2,161,692 + ABIE53_005851 0.34 -0.8 2,161,693 - ABIE53_005851 0.34 +0.2 2,161,751 + ABIE53_005851 0.67 +0.5 2,161,752 - ABIE53_005851 0.68 +0.4 2,161,752 - ABIE53_005851 0.68 -1.6 2,161,752 - ABIE53_005851 0.68 -0.3 2,161,765 + ABIE53_005851 0.75 -0.1 2,161,765 + ABIE53_005851 0.75 -0.5 2,161,766 - ABIE53_005851 0.76 -1.4 2,161,809 - -0.1 2,161,966 - ABIE53_005852 0.12 -0.0
Or see this region's nucleotide sequence