Experiment: R2A with 3-methyl-3-butenol 4.5 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000223 and ABIE53_000224 overlap by 14 nucleotides ABIE53_000224 and ABIE53_000225 are separated by 147 nucleotides
ABIE53_000223: ABIE53_000223 - hypothetical protein, at 227,565 to 227,804
_000223
ABIE53_000224: ABIE53_000224 - hypothetical protein, at 227,791 to 228,126
_000224
ABIE53_000225: ABIE53_000225 - hypothetical protein, at 228,274 to 228,849
_000225
Position (kb) 227
228
229 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 226.796 kb on + strand at 226.797 kb on - strand at 226.806 kb on + strand at 226.807 kb on - strand at 226.807 kb on - strand at 226.807 kb on - strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.836 kb on - strand at 226.836 kb on - strand at 226.838 kb on + strand at 226.838 kb on + strand at 226.839 kb on - strand at 226.839 kb on - strand at 226.878 kb on + strand at 226.879 kb on - strand at 226.879 kb on - strand at 226.929 kb on + strand at 226.930 kb on - strand at 226.930 kb on - strand at 226.965 kb on + strand at 227.046 kb on + strand at 227.047 kb on - strand at 227.047 kb on - strand at 227.107 kb on + strand at 227.108 kb on - strand at 227.108 kb on - strand at 227.114 kb on + strand at 227.114 kb on + strand at 227.114 kb on + strand at 227.115 kb on - strand at 227.115 kb on - strand at 227.115 kb on - strand at 227.156 kb on - strand at 227.156 kb on - strand at 227.156 kb on - strand at 227.166 kb on - strand at 227.166 kb on - strand at 227.169 kb on + strand at 227.169 kb on + strand at 227.169 kb on + strand at 227.169 kb on + strand at 227.169 kb on + strand at 227.170 kb on - strand at 227.170 kb on - strand at 227.170 kb on - strand at 227.170 kb on - strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.333 kb on + strand at 227.333 kb on + strand at 227.334 kb on - strand at 227.334 kb on - strand at 227.371 kb on + strand at 227.371 kb on + strand at 227.372 kb on - strand at 227.372 kb on - strand at 227.416 kb on + strand at 227.417 kb on - strand at 227.417 kb on - strand at 227.460 kb on + strand at 227.460 kb on + strand at 227.461 kb on - strand at 227.468 kb on + strand at 227.468 kb on + strand at 227.468 kb on + strand at 227.468 kb on + strand at 227.468 kb on + strand at 227.469 kb on - strand at 227.469 kb on - strand at 227.514 kb on + strand at 227.515 kb on - strand at 227.559 kb on + strand at 227.559 kb on + strand at 227.559 kb on + strand at 227.559 kb on + strand at 227.560 kb on - strand at 227.560 kb on - strand at 227.658 kb on - strand, within ABIE53_000223 at 227.658 kb on - strand, within ABIE53_000223 at 227.674 kb on + strand, within ABIE53_000223 at 227.709 kb on - strand, within ABIE53_000223 at 227.745 kb on + strand, within ABIE53_000223 at 227.745 kb on + strand, within ABIE53_000223 at 227.746 kb on - strand, within ABIE53_000223 at 227.746 kb on - strand, within ABIE53_000223 at 227.746 kb on - strand, within ABIE53_000223 at 227.843 kb on + strand, within ABIE53_000224 at 227.843 kb on + strand, within ABIE53_000224 at 227.843 kb on + strand, within ABIE53_000224 at 227.843 kb on + strand, within ABIE53_000224 at 227.843 kb on + strand, within ABIE53_000224 at 227.843 kb on + strand, within ABIE53_000224 at 227.843 kb on + strand, within ABIE53_000224 at 227.843 kb on + strand, within ABIE53_000224 at 227.843 kb on + strand, within ABIE53_000224 at 227.844 kb on - strand, within ABIE53_000224 at 227.844 kb on - strand, within ABIE53_000224 at 227.844 kb on - strand, within ABIE53_000224 at 227.844 kb on - strand, within ABIE53_000224 at 227.844 kb on - strand, within ABIE53_000224 at 227.881 kb on + strand, within ABIE53_000224 at 227.881 kb on + strand, within ABIE53_000224 at 227.881 kb on + strand, within ABIE53_000224 at 227.881 kb on + strand, within ABIE53_000224 at 227.881 kb on + strand, within ABIE53_000224 at 227.882 kb on - strand, within ABIE53_000224 at 227.882 kb on - strand, within ABIE53_000224 at 227.882 kb on - strand, within ABIE53_000224 at 227.882 kb on - strand, within ABIE53_000224 at 227.882 kb on - strand, within ABIE53_000224 at 227.882 kb on - strand, within ABIE53_000224 at 227.882 kb on - strand, within ABIE53_000224 at 227.885 kb on + strand, within ABIE53_000224 at 227.885 kb on + strand, within ABIE53_000224 at 227.886 kb on - strand, within ABIE53_000224 at 227.886 kb on - strand, within ABIE53_000224 at 227.927 kb on + strand, within ABIE53_000224 at 227.927 kb on + strand, within ABIE53_000224 at 227.928 kb on - strand, within ABIE53_000224 at 227.970 kb on - strand, within ABIE53_000224 at 227.970 kb on - strand, within ABIE53_000224 at 227.970 kb on - strand, within ABIE53_000224 at 228.005 kb on + strand, within ABIE53_000224 at 228.005 kb on + strand, within ABIE53_000224 at 228.005 kb on + strand, within ABIE53_000224 at 228.006 kb on - strand, within ABIE53_000224 at 228.006 kb on - strand, within ABIE53_000224 at 228.006 kb on - strand, within ABIE53_000224 at 228.018 kb on + strand, within ABIE53_000224 at 228.101 kb on - strand at 228.152 kb on + strand at 228.152 kb on + strand at 228.164 kb on + strand at 228.164 kb on + strand at 228.170 kb on + strand at 228.170 kb on + strand at 228.171 kb on - strand at 228.275 kb on + strand at 228.276 kb on - strand at 228.277 kb on + strand at 228.277 kb on + strand at 228.295 kb on - strand at 228.295 kb on - strand at 228.675 kb on + strand, within ABIE53_000225 at 228.675 kb on + strand, within ABIE53_000225 at 228.676 kb on - strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.690 kb on + strand, within ABIE53_000225 at 228.691 kb on - strand, within ABIE53_000225 at 228.691 kb on - strand, within ABIE53_000225 at 228.691 kb on - strand, within ABIE53_000225 at 228.691 kb on - strand, within ABIE53_000225 at 228.691 kb on - strand, within ABIE53_000225 at 228.755 kb on + strand, within ABIE53_000225 at 228.755 kb on + strand, within ABIE53_000225 at 228.756 kb on - strand, within ABIE53_000225 at 228.756 kb on - strand, within ABIE53_000225 at 228.867 kb on - strand at 228.919 kb on + strand at 228.920 kb on - strand at 228.920 kb on - strand at 228.949 kb on - strand at 228.949 kb on - strand at 228.949 kb on - strand at 229.028 kb on + strand at 229.028 kb on + strand at 229.028 kb on + strand at 229.028 kb on + strand at 229.029 kb on - strand at 229.029 kb on - strand at 229.122 kb on + strand at 229.122 kb on + strand at 229.123 kb on - strand at 229.123 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-methyl-3-butenol 4.5 g/L remove 226,796 + -0.4 226,797 - -0.2 226,806 + +0.7 226,807 - -0.3 226,807 - +0.1 226,807 - -1.4 226,835 + -0.3 226,835 + -1.5 226,835 + -1.0 226,835 + -2.0 226,835 + -0.4 226,836 - -1.2 226,836 - -0.3 226,838 + -0.6 226,838 + -0.5 226,839 - -1.7 226,839 - +0.4 226,878 + +0.5 226,879 - +0.5 226,879 - -0.2 226,929 + -0.4 226,930 - +1.3 226,930 - -0.5 226,965 + +0.1 227,046 + -0.3 227,047 - +0.1 227,047 - +1.1 227,107 + +0.2 227,108 - +0.1 227,108 - +0.2 227,114 + -0.4 227,114 + -0.4 227,114 + +1.6 227,115 - +2.0 227,115 - +0.3 227,115 - -0.5 227,156 - -0.2 227,156 - +0.1 227,156 - -0.3 227,166 - +2.1 227,166 - -0.8 227,169 + -0.4 227,169 + -1.8 227,169 + -1.2 227,169 + -1.4 227,169 + -0.9 227,170 - -1.1 227,170 - +0.9 227,170 - -0.2 227,170 - +0.2 227,285 + +1.0 227,285 + +0.6 227,285 + -1.5 227,285 + +0.1 227,285 + -1.2 227,285 + +0.9 227,285 + -1.0 227,285 + -0.7 227,285 + -0.2 227,286 - +0.4 227,286 - +0.0 227,286 - -1.5 227,286 - -0.2 227,286 - -0.3 227,286 - +0.3 227,286 - +0.1 227,286 - -1.5 227,286 - -0.9 227,286 - -0.1 227,333 + -0.7 227,333 + -0.4 227,334 - +0.2 227,334 - -0.2 227,371 + +0.9 227,371 + -0.4 227,372 - +0.1 227,372 - -0.8 227,416 + -0.2 227,417 - -2.1 227,417 - +0.2 227,460 + -0.1 227,460 + +0.8 227,461 - +0.2 227,468 + -0.3 227,468 + -0.7 227,468 + -0.1 227,468 + -0.5 227,468 + +0.3 227,469 - -0.8 227,469 - -0.3 227,514 + +0.1 227,515 - -0.2 227,559 + -0.6 227,559 + -0.6 227,559 + -0.7 227,559 + +0.0 227,560 - +0.6 227,560 - -0.1 227,658 - ABIE53_000223 0.39 +2.2 227,658 - ABIE53_000223 0.39 -0.6 227,674 + ABIE53_000223 0.45 +1.4 227,709 - ABIE53_000223 0.60 +0.1 227,745 + ABIE53_000223 0.75 +0.6 227,745 + ABIE53_000223 0.75 -1.2 227,746 - ABIE53_000223 0.75 -1.2 227,746 - ABIE53_000223 0.75 -0.7 227,746 - ABIE53_000223 0.75 -1.2 227,843 + ABIE53_000224 0.15 -1.3 227,843 + ABIE53_000224 0.15 +0.2 227,843 + ABIE53_000224 0.15 -0.3 227,843 + ABIE53_000224 0.15 +0.2 227,843 + ABIE53_000224 0.15 -0.4 227,843 + ABIE53_000224 0.15 +1.4 227,843 + ABIE53_000224 0.15 -0.5 227,843 + ABIE53_000224 0.15 -2.6 227,843 + ABIE53_000224 0.15 -0.1 227,844 - ABIE53_000224 0.16 +0.3 227,844 - ABIE53_000224 0.16 -0.2 227,844 - ABIE53_000224 0.16 +0.7 227,844 - ABIE53_000224 0.16 +1.9 227,844 - ABIE53_000224 0.16 -0.1 227,881 + ABIE53_000224 0.27 +0.5 227,881 + ABIE53_000224 0.27 -0.5 227,881 + ABIE53_000224 0.27 +0.0 227,881 + ABIE53_000224 0.27 +0.1 227,881 + ABIE53_000224 0.27 +1.0 227,882 - ABIE53_000224 0.27 -0.5 227,882 - ABIE53_000224 0.27 -0.2 227,882 - ABIE53_000224 0.27 +0.8 227,882 - ABIE53_000224 0.27 +0.1 227,882 - ABIE53_000224 0.27 +0.8 227,882 - ABIE53_000224 0.27 -0.1 227,882 - ABIE53_000224 0.27 -0.3 227,885 + ABIE53_000224 0.28 -0.1 227,885 + ABIE53_000224 0.28 -0.0 227,886 - ABIE53_000224 0.28 -0.6 227,886 - ABIE53_000224 0.28 -0.8 227,927 + ABIE53_000224 0.40 -0.6 227,927 + ABIE53_000224 0.40 -0.6 227,928 - ABIE53_000224 0.41 -0.1 227,970 - ABIE53_000224 0.53 -0.5 227,970 - ABIE53_000224 0.53 -0.6 227,970 - ABIE53_000224 0.53 +1.1 228,005 + ABIE53_000224 0.64 +0.3 228,005 + ABIE53_000224 0.64 +0.0 228,005 + ABIE53_000224 0.64 +2.9 228,006 - ABIE53_000224 0.64 -0.4 228,006 - ABIE53_000224 0.64 +0.5 228,006 - ABIE53_000224 0.64 +1.3 228,018 + ABIE53_000224 0.68 -0.7 228,101 - -0.5 228,152 + -0.2 228,152 + -0.3 228,164 + -0.8 228,164 + -1.4 228,170 + -0.3 228,170 + +2.0 228,171 - +1.0 228,275 + -0.1 228,276 - -1.6 228,277 + +0.3 228,277 + +0.6 228,295 - +0.3 228,295 - -1.0 228,675 + ABIE53_000225 0.70 +0.5 228,675 + ABIE53_000225 0.70 -0.6 228,676 - ABIE53_000225 0.70 +1.3 228,690 + ABIE53_000225 0.72 -0.4 228,690 + ABIE53_000225 0.72 -0.2 228,690 + ABIE53_000225 0.72 -0.6 228,690 + ABIE53_000225 0.72 -1.0 228,690 + ABIE53_000225 0.72 +1.9 228,690 + ABIE53_000225 0.72 +1.1 228,690 + ABIE53_000225 0.72 -0.6 228,690 + ABIE53_000225 0.72 -0.9 228,690 + ABIE53_000225 0.72 +0.2 228,691 - ABIE53_000225 0.72 -0.5 228,691 - ABIE53_000225 0.72 -1.8 228,691 - ABIE53_000225 0.72 -0.4 228,691 - ABIE53_000225 0.72 +0.4 228,691 - ABIE53_000225 0.72 +0.3 228,755 + ABIE53_000225 0.84 -1.6 228,755 + ABIE53_000225 0.84 +0.6 228,756 - ABIE53_000225 0.84 -0.4 228,756 - ABIE53_000225 0.84 -0.3 228,867 - -0.8 228,919 + -1.4 228,920 - -0.4 228,920 - +0.3 228,949 - -0.6 228,949 - -0.0 228,949 - -0.1 229,028 + -1.3 229,028 + +0.6 229,028 + -2.2 229,028 + -0.5 229,029 - +0.2 229,029 - -0.3 229,122 + +1.5 229,122 + -0.5 229,123 - -0.1 229,123 - +0.9
Or see this region's nucleotide sequence