Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS20410 and HMPREF1058_RS17975 are separated by 148 nucleotides HMPREF1058_RS17975 and HMPREF1058_RS17970 are separated by 14 nucleotides HMPREF1058_RS17970 and HMPREF1058_RS17965 are separated by 11 nucleotides HMPREF1058_RS17965 and HMPREF1058_RS17960 overlap by 4 nucleotides
HMPREF1058_RS20410: HMPREF1058_RS20410 - hypothetical protein, at 288,443 to 288,675
_RS20410
HMPREF1058_RS17975: HMPREF1058_RS17975 - ATP-dependent dethiobiotin synthetase BioD, at 288,824 to 289,468
_RS17975
HMPREF1058_RS17970: HMPREF1058_RS17970 - malonyl-ACP O-methyltransferase BioC, at 289,483 to 290,238
_RS17970
HMPREF1058_RS17965: HMPREF1058_RS17965 - DUF452 family protein, at 290,250 to 290,903
_RS17965
HMPREF1058_RS17960: HMPREF1058_RS17960 - 8-amino-7-oxononanoate synthase, at 290,900 to 292,051
_RS17960
Position (kb) 289
290
291 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 288.490 kb on + strand, within HMPREF1058_RS20410 at 288.490 kb on + strand, within HMPREF1058_RS20410 at 288.491 kb on - strand, within HMPREF1058_RS20410 at 288.493 kb on + strand, within HMPREF1058_RS20410 at 288.520 kb on - strand, within HMPREF1058_RS20410 at 288.624 kb on + strand, within HMPREF1058_RS20410 at 288.625 kb on - strand, within HMPREF1058_RS20410 at 288.652 kb on + strand at 288.652 kb on + strand at 288.652 kb on + strand at 288.673 kb on + strand at 288.719 kb on + strand at 288.719 kb on + strand at 288.721 kb on + strand at 288.722 kb on - strand at 288.726 kb on + strand at 288.727 kb on - strand at 288.744 kb on + strand at 288.745 kb on - strand at 288.773 kb on + strand at 288.836 kb on + strand at 288.875 kb on - strand at 288.887 kb on - strand at 288.899 kb on - strand, within HMPREF1058_RS17975 at 288.899 kb on - strand, within HMPREF1058_RS17975 at 288.942 kb on + strand, within HMPREF1058_RS17975 at 288.942 kb on + strand, within HMPREF1058_RS17975 at 288.951 kb on + strand, within HMPREF1058_RS17975 at 288.952 kb on + strand, within HMPREF1058_RS17975 at 288.977 kb on + strand, within HMPREF1058_RS17975 at 288.978 kb on - strand, within HMPREF1058_RS17975 at 289.000 kb on + strand, within HMPREF1058_RS17975 at 289.009 kb on + strand, within HMPREF1058_RS17975 at 289.009 kb on + strand, within HMPREF1058_RS17975 at 289.065 kb on + strand, within HMPREF1058_RS17975 at 289.070 kb on + strand, within HMPREF1058_RS17975 at 289.071 kb on - strand, within HMPREF1058_RS17975 at 289.081 kb on + strand, within HMPREF1058_RS17975 at 289.122 kb on - strand, within HMPREF1058_RS17975 at 289.138 kb on - strand, within HMPREF1058_RS17975 at 289.278 kb on + strand, within HMPREF1058_RS17975 at 289.279 kb on - strand, within HMPREF1058_RS17975 at 289.363 kb on + strand, within HMPREF1058_RS17975 at 289.367 kb on - strand, within HMPREF1058_RS17975 at 289.454 kb on + strand at 289.507 kb on - strand at 289.508 kb on + strand at 289.509 kb on - strand at 289.512 kb on + strand at 289.512 kb on + strand at 289.709 kb on + strand, within HMPREF1058_RS17970 at 289.710 kb on - strand, within HMPREF1058_RS17970 at 289.846 kb on - strand, within HMPREF1058_RS17970 at 289.848 kb on + strand, within HMPREF1058_RS17970 at 289.849 kb on - strand, within HMPREF1058_RS17970 at 289.904 kb on - strand, within HMPREF1058_RS17970 at 290.017 kb on - strand, within HMPREF1058_RS17970 at 290.123 kb on + strand, within HMPREF1058_RS17970 at 290.124 kb on - strand, within HMPREF1058_RS17970 at 290.132 kb on - strand, within HMPREF1058_RS17970 at 290.155 kb on + strand, within HMPREF1058_RS17970 at 290.230 kb on - strand at 290.251 kb on + strand at 290.306 kb on + strand at 290.307 kb on - strand at 290.383 kb on - strand, within HMPREF1058_RS17965 at 290.657 kb on + strand, within HMPREF1058_RS17965 at 290.924 kb on + strand at 291.012 kb on + strand at 291.095 kb on - strand, within HMPREF1058_RS17960
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 288,490 + HMPREF1058_RS20410 0.20 +2.6 288,490 + HMPREF1058_RS20410 0.20 +0.9 288,491 - HMPREF1058_RS20410 0.21 -1.0 288,493 + HMPREF1058_RS20410 0.21 -0.9 288,520 - HMPREF1058_RS20410 0.33 +0.5 288,624 + HMPREF1058_RS20410 0.78 -1.3 288,625 - HMPREF1058_RS20410 0.78 -0.5 288,652 + -0.4 288,652 + -1.9 288,652 + -2.8 288,673 + -0.8 288,719 + +0.0 288,719 + -2.3 288,721 + -2.5 288,722 - -1.0 288,726 + -2.3 288,727 - -1.8 288,744 + +1.2 288,745 - -1.2 288,773 + +1.9 288,836 + -3.7 288,875 - -2.5 288,887 - -3.6 288,899 - HMPREF1058_RS17975 0.12 -1.6 288,899 - HMPREF1058_RS17975 0.12 -3.3 288,942 + HMPREF1058_RS17975 0.18 -4.6 288,942 + HMPREF1058_RS17975 0.18 -2.7 288,951 + HMPREF1058_RS17975 0.20 -3.7 288,952 + HMPREF1058_RS17975 0.20 -3.7 288,977 + HMPREF1058_RS17975 0.24 -3.8 288,978 - HMPREF1058_RS17975 0.24 -2.6 289,000 + HMPREF1058_RS17975 0.27 -3.0 289,009 + HMPREF1058_RS17975 0.29 -4.4 289,009 + HMPREF1058_RS17975 0.29 -2.6 289,065 + HMPREF1058_RS17975 0.37 -2.3 289,070 + HMPREF1058_RS17975 0.38 -4.9 289,071 - HMPREF1058_RS17975 0.38 -4.8 289,081 + HMPREF1058_RS17975 0.40 -5.1 289,122 - HMPREF1058_RS17975 0.46 -4.1 289,138 - HMPREF1058_RS17975 0.49 -1.8 289,278 + HMPREF1058_RS17975 0.70 -2.0 289,279 - HMPREF1058_RS17975 0.71 -2.4 289,363 + HMPREF1058_RS17975 0.84 -1.1 289,367 - HMPREF1058_RS17975 0.84 -2.7 289,454 + -2.6 289,507 - -2.4 289,508 + -3.4 289,509 - -1.8 289,512 + -3.6 289,512 + -1.3 289,709 + HMPREF1058_RS17970 0.30 -3.3 289,710 - HMPREF1058_RS17970 0.30 -1.6 289,846 - HMPREF1058_RS17970 0.48 -3.9 289,848 + HMPREF1058_RS17970 0.48 -4.1 289,849 - HMPREF1058_RS17970 0.48 -3.7 289,904 - HMPREF1058_RS17970 0.56 -4.8 290,017 - HMPREF1058_RS17970 0.71 -0.7 290,123 + HMPREF1058_RS17970 0.85 -3.0 290,124 - HMPREF1058_RS17970 0.85 -1.6 290,132 - HMPREF1058_RS17970 0.86 -4.5 290,155 + HMPREF1058_RS17970 0.89 -2.1 290,230 - -2.2 290,251 + -2.8 290,306 + +0.0 290,307 - -0.5 290,383 - HMPREF1058_RS17965 0.20 -2.7 290,657 + HMPREF1058_RS17965 0.62 -3.0 290,924 + -1.5 291,012 + -2.9 291,095 - HMPREF1058_RS17960 0.17 -2.3
Or see this region's nucleotide sequence