Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS14395 and HMPREF1058_RS21430 are separated by 9 nucleotides HMPREF1058_RS21430 and HMPREF1058_RS14390 are separated by 102 nucleotides HMPREF1058_RS14390 and HMPREF1058_RS14385 are separated by 5 nucleotides HMPREF1058_RS14385 and HMPREF1058_RS14380 are separated by 21 nucleotides HMPREF1058_RS14380 and HMPREF1058_RS14375 are separated by 82 nucleotides
HMPREF1058_RS14395: HMPREF1058_RS14395 - HAD family hydrolase, at 1,213,648 to 1,214,301
_RS14395
HMPREF1058_RS21430: HMPREF1058_RS21430 - 6-phosphofructokinase, at 1,214,311 to 1,214,436
_RS21430
HMPREF1058_RS14390: HMPREF1058_RS14390 - DoxX family protein, at 1,214,539 to 1,214,979
_RS14390
HMPREF1058_RS14385: HMPREF1058_RS14385 - DUF4435 domain-containing protein, at 1,214,985 to 1,215,980
_RS14385
HMPREF1058_RS14380: HMPREF1058_RS14380 - AAA family ATPase, at 1,216,002 to 1,216,823
_RS14380
HMPREF1058_RS14375: HMPREF1058_RS14375 - DMT family transporter, at 1,216,906 to 1,217,832
_RS14375
Position (kb) 1214
1215
1216 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 1214.045 kb on + strand, within HMPREF1058_RS14395 at 1214.046 kb on - strand, within HMPREF1058_RS14395 at 1214.133 kb on + strand at 1214.134 kb on - strand, within HMPREF1058_RS14395 at 1214.135 kb on + strand, within HMPREF1058_RS14395 at 1214.141 kb on - strand, within HMPREF1058_RS14395 at 1214.154 kb on + strand, within HMPREF1058_RS14395 at 1214.155 kb on - strand, within HMPREF1058_RS14395 at 1214.160 kb on - strand, within HMPREF1058_RS14395 at 1214.160 kb on - strand, within HMPREF1058_RS14395 at 1214.160 kb on - strand, within HMPREF1058_RS14395 at 1214.189 kb on + strand, within HMPREF1058_RS14395 at 1214.198 kb on + strand, within HMPREF1058_RS14395 at 1214.198 kb on + strand, within HMPREF1058_RS14395 at 1214.198 kb on + strand, within HMPREF1058_RS14395 at 1214.198 kb on + strand, within HMPREF1058_RS14395 at 1214.199 kb on - strand, within HMPREF1058_RS14395 at 1214.262 kb on - strand at 1214.262 kb on - strand at 1214.316 kb on + strand at 1214.391 kb on + strand, within HMPREF1058_RS21430 at 1214.392 kb on - strand, within HMPREF1058_RS21430 at 1214.394 kb on - strand, within HMPREF1058_RS21430 at 1214.394 kb on - strand, within HMPREF1058_RS21430 at 1214.396 kb on - strand, within HMPREF1058_RS21430 at 1214.505 kb on - strand at 1214.514 kb on - strand at 1214.562 kb on - strand at 1214.589 kb on + strand, within HMPREF1058_RS14390 at 1214.633 kb on + strand, within HMPREF1058_RS14390 at 1214.633 kb on + strand, within HMPREF1058_RS14390 at 1214.634 kb on - strand, within HMPREF1058_RS14390 at 1214.708 kb on + strand, within HMPREF1058_RS14390 at 1214.873 kb on + strand, within HMPREF1058_RS14390 at 1214.894 kb on + strand at 1214.894 kb on + strand, within HMPREF1058_RS14390 at 1214.895 kb on - strand, within HMPREF1058_RS14390 at 1214.895 kb on - strand, within HMPREF1058_RS14390 at 1214.895 kb on - strand, within HMPREF1058_RS14390 at 1214.901 kb on - strand, within HMPREF1058_RS14390 at 1214.903 kb on + strand, within HMPREF1058_RS14390 at 1214.908 kb on + strand, within HMPREF1058_RS14390 at 1214.908 kb on + strand, within HMPREF1058_RS14390 at 1214.908 kb on + strand, within HMPREF1058_RS14390 at 1214.908 kb on + strand, within HMPREF1058_RS14390 at 1214.908 kb on + strand, within HMPREF1058_RS14390 at 1214.909 kb on - strand, within HMPREF1058_RS14390 at 1214.990 kb on + strand at 1215.009 kb on + strand at 1215.010 kb on - strand at 1215.047 kb on + strand at 1215.048 kb on - strand at 1215.048 kb on - strand at 1215.048 kb on - strand at 1215.078 kb on + strand at 1215.128 kb on + strand, within HMPREF1058_RS14385 at 1215.132 kb on - strand, within HMPREF1058_RS14385 at 1215.132 kb on - strand, within HMPREF1058_RS14385 at 1215.132 kb on - strand, within HMPREF1058_RS14385 at 1215.132 kb on - strand, within HMPREF1058_RS14385 at 1215.193 kb on - strand, within HMPREF1058_RS14385 at 1215.195 kb on + strand, within HMPREF1058_RS14385 at 1215.210 kb on + strand, within HMPREF1058_RS14385 at 1215.215 kb on + strand, within HMPREF1058_RS14385 at 1215.245 kb on + strand, within HMPREF1058_RS14385 at 1215.246 kb on - strand, within HMPREF1058_RS14385 at 1215.247 kb on + strand, within HMPREF1058_RS14385 at 1215.251 kb on + strand, within HMPREF1058_RS14385 at 1215.251 kb on + strand, within HMPREF1058_RS14385 at 1215.251 kb on + strand, within HMPREF1058_RS14385 at 1215.251 kb on + strand, within HMPREF1058_RS14385 at 1215.300 kb on + strand, within HMPREF1058_RS14385 at 1215.301 kb on - strand, within HMPREF1058_RS14385 at 1215.369 kb on - strand, within HMPREF1058_RS14385 at 1215.395 kb on - strand, within HMPREF1058_RS14385 at 1215.422 kb on - strand, within HMPREF1058_RS14385 at 1215.504 kb on + strand, within HMPREF1058_RS14385 at 1215.514 kb on + strand, within HMPREF1058_RS14385 at 1215.524 kb on + strand, within HMPREF1058_RS14385 at 1215.598 kb on + strand, within HMPREF1058_RS14385 at 1215.622 kb on + strand, within HMPREF1058_RS14385 at 1215.622 kb on + strand, within HMPREF1058_RS14385 at 1215.622 kb on + strand, within HMPREF1058_RS14385 at 1215.622 kb on + strand, within HMPREF1058_RS14385 at 1215.622 kb on + strand, within HMPREF1058_RS14385 at 1215.623 kb on - strand at 1215.623 kb on - strand, within HMPREF1058_RS14385 at 1215.628 kb on + strand, within HMPREF1058_RS14385 at 1215.628 kb on + strand at 1215.629 kb on - strand, within HMPREF1058_RS14385 at 1215.629 kb on - strand, within HMPREF1058_RS14385 at 1215.654 kb on + strand, within HMPREF1058_RS14385 at 1215.654 kb on + strand, within HMPREF1058_RS14385 at 1215.657 kb on + strand, within HMPREF1058_RS14385 at 1215.657 kb on + strand, within HMPREF1058_RS14385 at 1215.657 kb on + strand, within HMPREF1058_RS14385 at 1215.658 kb on - strand, within HMPREF1058_RS14385 at 1215.659 kb on + strand, within HMPREF1058_RS14385 at 1215.721 kb on - strand, within HMPREF1058_RS14385 at 1215.726 kb on - strand, within HMPREF1058_RS14385 at 1215.866 kb on + strand, within HMPREF1058_RS14385 at 1215.875 kb on - strand, within HMPREF1058_RS14385 at 1215.886 kb on + strand at 1215.887 kb on - strand at 1215.943 kb on + strand at 1215.943 kb on + strand at 1215.945 kb on + strand at 1215.946 kb on - strand at 1215.983 kb on + strand at 1216.013 kb on + strand at 1216.013 kb on + strand at 1216.014 kb on - strand at 1216.090 kb on - strand, within HMPREF1058_RS14380 at 1216.121 kb on + strand, within HMPREF1058_RS14380 at 1216.121 kb on + strand at 1216.369 kb on + strand, within HMPREF1058_RS14380 at 1216.453 kb on + strand, within HMPREF1058_RS14380 at 1216.566 kb on - strand, within HMPREF1058_RS14380 at 1216.800 kb on + strand at 1216.803 kb on - strand at 1216.803 kb on - strand at 1216.804 kb on + strand at 1216.804 kb on + strand at 1216.804 kb on + strand at 1216.805 kb on - strand at 1216.836 kb on + strand at 1216.837 kb on - strand at 1216.843 kb on - strand at 1216.898 kb on + strand at 1216.921 kb on + strand at 1216.922 kb on - strand at 1216.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 1,214,045 + HMPREF1058_RS14395 0.61 -1.2 1,214,046 - HMPREF1058_RS14395 0.61 -1.2 1,214,133 + -0.2 1,214,134 - HMPREF1058_RS14395 0.74 -0.1 1,214,135 + HMPREF1058_RS14395 0.74 +0.2 1,214,141 - HMPREF1058_RS14395 0.75 -0.4 1,214,154 + HMPREF1058_RS14395 0.77 -0.8 1,214,155 - HMPREF1058_RS14395 0.78 -0.7 1,214,160 - HMPREF1058_RS14395 0.78 -1.2 1,214,160 - HMPREF1058_RS14395 0.78 -0.9 1,214,160 - HMPREF1058_RS14395 0.78 +0.1 1,214,189 + HMPREF1058_RS14395 0.83 -0.5 1,214,198 + HMPREF1058_RS14395 0.84 -1.6 1,214,198 + HMPREF1058_RS14395 0.84 -0.6 1,214,198 + HMPREF1058_RS14395 0.84 -0.6 1,214,198 + HMPREF1058_RS14395 0.84 -0.8 1,214,199 - HMPREF1058_RS14395 0.84 -1.3 1,214,262 - +0.5 1,214,262 - -0.8 1,214,316 + +0.5 1,214,391 + HMPREF1058_RS21430 0.63 +2.8 1,214,392 - HMPREF1058_RS21430 0.64 +0.3 1,214,394 - HMPREF1058_RS21430 0.66 +0.9 1,214,394 - HMPREF1058_RS21430 0.66 +0.6 1,214,396 - HMPREF1058_RS21430 0.67 -0.8 1,214,505 - -0.6 1,214,514 - +0.2 1,214,562 - -1.9 1,214,589 + HMPREF1058_RS14390 0.11 +1.7 1,214,633 + HMPREF1058_RS14390 0.21 +0.0 1,214,633 + HMPREF1058_RS14390 0.21 +1.3 1,214,634 - HMPREF1058_RS14390 0.22 +1.5 1,214,708 + HMPREF1058_RS14390 0.38 -0.6 1,214,873 + HMPREF1058_RS14390 0.76 -0.2 1,214,894 + +0.3 1,214,894 + HMPREF1058_RS14390 0.80 +0.6 1,214,895 - HMPREF1058_RS14390 0.81 +0.0 1,214,895 - HMPREF1058_RS14390 0.81 -0.4 1,214,895 - HMPREF1058_RS14390 0.81 -0.6 1,214,901 - HMPREF1058_RS14390 0.82 -1.4 1,214,903 + HMPREF1058_RS14390 0.83 -1.4 1,214,908 + HMPREF1058_RS14390 0.84 +1.7 1,214,908 + HMPREF1058_RS14390 0.84 -1.1 1,214,908 + HMPREF1058_RS14390 0.84 +0.2 1,214,908 + HMPREF1058_RS14390 0.84 +0.9 1,214,908 + HMPREF1058_RS14390 0.84 +1.7 1,214,909 - HMPREF1058_RS14390 0.84 +1.7 1,214,990 + -2.0 1,215,009 + -5.7 1,215,010 - -3.3 1,215,047 + -3.4 1,215,048 - -4.3 1,215,048 - -4.7 1,215,048 - -5.1 1,215,078 + -2.1 1,215,128 + HMPREF1058_RS14385 0.14 -3.4 1,215,132 - HMPREF1058_RS14385 0.15 -2.8 1,215,132 - HMPREF1058_RS14385 0.15 -3.7 1,215,132 - HMPREF1058_RS14385 0.15 -4.9 1,215,132 - HMPREF1058_RS14385 0.15 -4.4 1,215,193 - HMPREF1058_RS14385 0.21 -5.6 1,215,195 + HMPREF1058_RS14385 0.21 -4.4 1,215,210 + HMPREF1058_RS14385 0.23 -1.7 1,215,215 + HMPREF1058_RS14385 0.23 -4.4 1,215,245 + HMPREF1058_RS14385 0.26 -0.7 1,215,246 - HMPREF1058_RS14385 0.26 -2.1 1,215,247 + HMPREF1058_RS14385 0.26 -2.8 1,215,251 + HMPREF1058_RS14385 0.27 -5.3 1,215,251 + HMPREF1058_RS14385 0.27 -4.2 1,215,251 + HMPREF1058_RS14385 0.27 -5.2 1,215,251 + HMPREF1058_RS14385 0.27 -0.2 1,215,300 + HMPREF1058_RS14385 0.32 -3.7 1,215,301 - HMPREF1058_RS14385 0.32 -3.4 1,215,369 - HMPREF1058_RS14385 0.39 -4.8 1,215,395 - HMPREF1058_RS14385 0.41 -2.1 1,215,422 - HMPREF1058_RS14385 0.44 -6.3 1,215,504 + HMPREF1058_RS14385 0.52 -4.6 1,215,514 + HMPREF1058_RS14385 0.53 -3.1 1,215,524 + HMPREF1058_RS14385 0.54 -5.0 1,215,598 + HMPREF1058_RS14385 0.62 -4.3 1,215,622 + HMPREF1058_RS14385 0.64 -3.6 1,215,622 + HMPREF1058_RS14385 0.64 -2.4 1,215,622 + HMPREF1058_RS14385 0.64 -3.9 1,215,622 + HMPREF1058_RS14385 0.64 -5.1 1,215,622 + HMPREF1058_RS14385 0.64 -1.9 1,215,623 - -0.6 1,215,623 - HMPREF1058_RS14385 0.64 -2.1 1,215,628 + HMPREF1058_RS14385 0.65 -0.7 1,215,628 + -0.4 1,215,629 - HMPREF1058_RS14385 0.65 -1.5 1,215,629 - HMPREF1058_RS14385 0.65 -1.2 1,215,654 + HMPREF1058_RS14385 0.67 -5.2 1,215,654 + HMPREF1058_RS14385 0.67 -4.8 1,215,657 + HMPREF1058_RS14385 0.67 -1.3 1,215,657 + HMPREF1058_RS14385 0.67 -5.3 1,215,657 + HMPREF1058_RS14385 0.67 -4.7 1,215,658 - HMPREF1058_RS14385 0.68 -3.3 1,215,659 + HMPREF1058_RS14385 0.68 -1.9 1,215,721 - HMPREF1058_RS14385 0.74 -2.4 1,215,726 - HMPREF1058_RS14385 0.74 -3.4 1,215,866 + HMPREF1058_RS14385 0.88 -2.1 1,215,875 - HMPREF1058_RS14385 0.89 -4.2 1,215,886 + -0.9 1,215,887 - -2.6 1,215,943 + -2.1 1,215,943 + -1.7 1,215,945 + -4.7 1,215,946 - -1.6 1,215,983 + -4.2 1,216,013 + -3.1 1,216,013 + -3.4 1,216,014 - -4.7 1,216,090 - HMPREF1058_RS14380 0.11 -5.3 1,216,121 + HMPREF1058_RS14380 0.14 -1.9 1,216,121 + -1.6 1,216,369 + HMPREF1058_RS14380 0.45 -6.3 1,216,453 + HMPREF1058_RS14380 0.55 -5.0 1,216,566 - HMPREF1058_RS14380 0.69 -4.3 1,216,800 + -2.1 1,216,803 - -0.4 1,216,803 - -5.1 1,216,804 + -4.2 1,216,804 + -3.4 1,216,804 + -1.1 1,216,805 - -5.0 1,216,836 + -4.7 1,216,837 - +0.1 1,216,843 - +0.1 1,216,898 + -1.3 1,216,921 + -4.2 1,216,922 - -2.7 1,216,956 - -1.6
Or see this region's nucleotide sequence