Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06685 and HMPREF1058_RS06680 are separated by 3 nucleotides HMPREF1058_RS06680 and HMPREF1058_RS06675 are separated by 5 nucleotides HMPREF1058_RS06675 and HMPREF1058_RS06670 are separated by 36 nucleotides
HMPREF1058_RS06685: HMPREF1058_RS06685 - O-antigen ligase family protein, at 418,251 to 419,711
_RS06685
HMPREF1058_RS06680: HMPREF1058_RS06680 - hypothetical protein, at 419,715 to 421,862
_RS06680
HMPREF1058_RS06675: HMPREF1058_RS06675 - TolC family protein, at 421,868 to 422,731
_RS06675
HMPREF1058_RS06670: HMPREF1058_RS06670 - sugar transferase, at 422,768 to 423,943
_RS06670
Position (kb) 419
420
421
422 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 418.727 kb on + strand, within HMPREF1058_RS06685 at 418.763 kb on + strand, within HMPREF1058_RS06685 at 418.873 kb on - strand, within HMPREF1058_RS06685 at 418.930 kb on - strand, within HMPREF1058_RS06685 at 418.961 kb on + strand, within HMPREF1058_RS06685 at 419.044 kb on + strand, within HMPREF1058_RS06685 at 419.045 kb on - strand, within HMPREF1058_RS06685 at 419.083 kb on + strand, within HMPREF1058_RS06685 at 419.101 kb on - strand, within HMPREF1058_RS06685 at 419.217 kb on + strand, within HMPREF1058_RS06685 at 419.217 kb on + strand, within HMPREF1058_RS06685 at 419.218 kb on - strand, within HMPREF1058_RS06685 at 419.229 kb on - strand, within HMPREF1058_RS06685 at 419.229 kb on - strand, within HMPREF1058_RS06685 at 419.229 kb on - strand, within HMPREF1058_RS06685 at 419.305 kb on + strand, within HMPREF1058_RS06685 at 419.316 kb on + strand, within HMPREF1058_RS06685 at 419.317 kb on - strand, within HMPREF1058_RS06685 at 419.317 kb on - strand, within HMPREF1058_RS06685 at 419.360 kb on + strand, within HMPREF1058_RS06685 at 419.362 kb on + strand, within HMPREF1058_RS06685 at 419.363 kb on - strand at 419.453 kb on + strand, within HMPREF1058_RS06685 at 419.454 kb on - strand, within HMPREF1058_RS06685 at 419.460 kb on - strand, within HMPREF1058_RS06685 at 419.482 kb on + strand at 419.483 kb on - strand, within HMPREF1058_RS06685 at 419.490 kb on - strand, within HMPREF1058_RS06685 at 419.557 kb on - strand, within HMPREF1058_RS06685 at 419.557 kb on - strand, within HMPREF1058_RS06685 at 419.564 kb on + strand, within HMPREF1058_RS06685 at 419.688 kb on + strand at 419.690 kb on + strand at 419.691 kb on - strand at 419.713 kb on - strand at 419.808 kb on - strand at 419.822 kb on + strand at 419.880 kb on + strand at 419.881 kb on - strand at 419.921 kb on + strand at 420.045 kb on + strand, within HMPREF1058_RS06680 at 420.045 kb on + strand, within HMPREF1058_RS06680 at 420.046 kb on - strand, within HMPREF1058_RS06680 at 420.069 kb on + strand, within HMPREF1058_RS06680 at 420.073 kb on + strand, within HMPREF1058_RS06680 at 420.073 kb on + strand, within HMPREF1058_RS06680 at 420.073 kb on + strand, within HMPREF1058_RS06680 at 420.074 kb on - strand, within HMPREF1058_RS06680 at 420.090 kb on + strand, within HMPREF1058_RS06680 at 420.153 kb on + strand, within HMPREF1058_RS06680 at 420.194 kb on + strand, within HMPREF1058_RS06680 at 420.198 kb on + strand, within HMPREF1058_RS06680 at 420.227 kb on + strand, within HMPREF1058_RS06680 at 420.227 kb on + strand, within HMPREF1058_RS06680 at 420.231 kb on + strand, within HMPREF1058_RS06680 at 420.231 kb on + strand at 420.290 kb on + strand at 420.291 kb on - strand, within HMPREF1058_RS06680 at 420.291 kb on - strand, within HMPREF1058_RS06680 at 420.292 kb on + strand, within HMPREF1058_RS06680 at 420.293 kb on - strand, within HMPREF1058_RS06680 at 420.306 kb on + strand, within HMPREF1058_RS06680 at 420.399 kb on + strand, within HMPREF1058_RS06680 at 420.430 kb on - strand, within HMPREF1058_RS06680 at 420.454 kb on - strand, within HMPREF1058_RS06680 at 420.454 kb on - strand, within HMPREF1058_RS06680 at 420.587 kb on + strand, within HMPREF1058_RS06680 at 420.587 kb on + strand, within HMPREF1058_RS06680 at 420.588 kb on - strand, within HMPREF1058_RS06680 at 420.588 kb on - strand, within HMPREF1058_RS06680 at 420.588 kb on - strand, within HMPREF1058_RS06680 at 420.588 kb on - strand, within HMPREF1058_RS06680 at 420.590 kb on - strand, within HMPREF1058_RS06680 at 420.687 kb on + strand, within HMPREF1058_RS06680 at 420.690 kb on - strand, within HMPREF1058_RS06680 at 420.713 kb on + strand, within HMPREF1058_RS06680 at 420.771 kb on + strand at 420.771 kb on + strand, within HMPREF1058_RS06680 at 420.772 kb on - strand, within HMPREF1058_RS06680 at 420.843 kb on + strand, within HMPREF1058_RS06680 at 420.843 kb on + strand, within HMPREF1058_RS06680 at 420.844 kb on - strand, within HMPREF1058_RS06680 at 420.995 kb on + strand, within HMPREF1058_RS06680 at 420.996 kb on - strand, within HMPREF1058_RS06680 at 420.998 kb on - strand, within HMPREF1058_RS06680 at 421.004 kb on - strand, within HMPREF1058_RS06680 at 421.108 kb on + strand, within HMPREF1058_RS06680 at 421.128 kb on + strand, within HMPREF1058_RS06680 at 421.129 kb on - strand, within HMPREF1058_RS06680 at 421.284 kb on - strand, within HMPREF1058_RS06680 at 421.286 kb on - strand, within HMPREF1058_RS06680 at 421.290 kb on + strand, within HMPREF1058_RS06680 at 421.312 kb on + strand, within HMPREF1058_RS06680 at 421.329 kb on - strand, within HMPREF1058_RS06680 at 421.329 kb on - strand, within HMPREF1058_RS06680 at 421.357 kb on - strand, within HMPREF1058_RS06680 at 421.377 kb on + strand, within HMPREF1058_RS06680 at 421.377 kb on + strand, within HMPREF1058_RS06680 at 421.462 kb on + strand, within HMPREF1058_RS06680 at 421.488 kb on + strand, within HMPREF1058_RS06680 at 421.489 kb on - strand, within HMPREF1058_RS06680 at 421.580 kb on - strand, within HMPREF1058_RS06680 at 421.611 kb on + strand, within HMPREF1058_RS06680 at 421.612 kb on - strand, within HMPREF1058_RS06680 at 421.614 kb on + strand, within HMPREF1058_RS06680 at 421.638 kb on - strand, within HMPREF1058_RS06680 at 421.651 kb on - strand at 421.695 kb on + strand at 421.695 kb on + strand at 421.696 kb on - strand at 421.744 kb on + strand at 421.745 kb on - strand at 421.766 kb on + strand at 421.779 kb on + strand at 421.793 kb on + strand at 421.793 kb on + strand at 421.794 kb on - strand at 421.794 kb on - strand at 421.794 kb on - strand at 421.803 kb on + strand at 421.804 kb on - strand at 421.829 kb on - strand at 421.829 kb on - strand at 421.956 kb on - strand, within HMPREF1058_RS06675 at 422.012 kb on - strand, within HMPREF1058_RS06675 at 422.012 kb on - strand, within HMPREF1058_RS06675 at 422.021 kb on + strand, within HMPREF1058_RS06675 at 422.062 kb on + strand, within HMPREF1058_RS06675 at 422.089 kb on + strand, within HMPREF1058_RS06675 at 422.102 kb on - strand, within HMPREF1058_RS06675 at 422.134 kb on + strand, within HMPREF1058_RS06675 at 422.137 kb on - strand, within HMPREF1058_RS06675 at 422.138 kb on + strand, within HMPREF1058_RS06675 at 422.138 kb on + strand, within HMPREF1058_RS06675 at 422.138 kb on + strand, within HMPREF1058_RS06675 at 422.138 kb on + strand, within HMPREF1058_RS06675 at 422.146 kb on + strand, within HMPREF1058_RS06675 at 422.196 kb on + strand, within HMPREF1058_RS06675 at 422.196 kb on + strand, within HMPREF1058_RS06675 at 422.197 kb on - strand, within HMPREF1058_RS06675 at 422.197 kb on - strand, within HMPREF1058_RS06675 at 422.198 kb on + strand, within HMPREF1058_RS06675 at 422.214 kb on - strand, within HMPREF1058_RS06675 at 422.229 kb on + strand, within HMPREF1058_RS06675 at 422.229 kb on + strand, within HMPREF1058_RS06675 at 422.363 kb on + strand, within HMPREF1058_RS06675 at 422.364 kb on - strand, within HMPREF1058_RS06675 at 422.367 kb on + strand, within HMPREF1058_RS06675 at 422.368 kb on - strand, within HMPREF1058_RS06675 at 422.368 kb on - strand, within HMPREF1058_RS06675 at 422.368 kb on - strand, within HMPREF1058_RS06675 at 422.452 kb on + strand, within HMPREF1058_RS06675 at 422.486 kb on + strand, within HMPREF1058_RS06675 at 422.499 kb on - strand, within HMPREF1058_RS06675 at 422.516 kb on + strand, within HMPREF1058_RS06675 at 422.517 kb on - strand, within HMPREF1058_RS06675 at 422.564 kb on + strand, within HMPREF1058_RS06675 at 422.603 kb on - strand, within HMPREF1058_RS06675 at 422.613 kb on - strand, within HMPREF1058_RS06675 at 422.615 kb on - strand, within HMPREF1058_RS06675 at 422.698 kb on - strand at 422.736 kb on + strand at 422.737 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 418,727 + HMPREF1058_RS06685 0.33 +1.8 418,763 + HMPREF1058_RS06685 0.35 -1.2 418,873 - HMPREF1058_RS06685 0.43 -2.4 418,930 - HMPREF1058_RS06685 0.46 -3.8 418,961 + HMPREF1058_RS06685 0.49 -2.1 419,044 + HMPREF1058_RS06685 0.54 -0.1 419,045 - HMPREF1058_RS06685 0.54 -0.8 419,083 + HMPREF1058_RS06685 0.57 +1.1 419,101 - HMPREF1058_RS06685 0.58 +1.8 419,217 + HMPREF1058_RS06685 0.66 +1.7 419,217 + HMPREF1058_RS06685 0.66 -0.4 419,218 - HMPREF1058_RS06685 0.66 -0.2 419,229 - HMPREF1058_RS06685 0.67 -1.2 419,229 - HMPREF1058_RS06685 0.67 +1.0 419,229 - HMPREF1058_RS06685 0.67 -3.9 419,305 + HMPREF1058_RS06685 0.72 +0.0 419,316 + HMPREF1058_RS06685 0.73 -1.3 419,317 - HMPREF1058_RS06685 0.73 +1.0 419,317 - HMPREF1058_RS06685 0.73 +1.2 419,360 + HMPREF1058_RS06685 0.76 +1.8 419,362 + HMPREF1058_RS06685 0.76 +2.6 419,363 - +0.3 419,453 + HMPREF1058_RS06685 0.82 +0.0 419,454 - HMPREF1058_RS06685 0.82 +1.6 419,460 - HMPREF1058_RS06685 0.83 -1.2 419,482 + -0.2 419,483 - HMPREF1058_RS06685 0.84 -2.8 419,490 - HMPREF1058_RS06685 0.85 -3.6 419,557 - HMPREF1058_RS06685 0.89 +0.2 419,557 - HMPREF1058_RS06685 0.89 -1.1 419,564 + HMPREF1058_RS06685 0.90 -0.8 419,688 + -0.3 419,690 + -0.2 419,691 - -1.6 419,713 - -0.5 419,808 - -0.2 419,822 + +2.0 419,880 + -2.2 419,881 - +1.2 419,921 + -0.4 420,045 + HMPREF1058_RS06680 0.15 -0.7 420,045 + HMPREF1058_RS06680 0.15 -3.5 420,046 - HMPREF1058_RS06680 0.15 -0.7 420,069 + HMPREF1058_RS06680 0.16 +1.0 420,073 + HMPREF1058_RS06680 0.17 -0.9 420,073 + HMPREF1058_RS06680 0.17 -0.4 420,073 + HMPREF1058_RS06680 0.17 +1.5 420,074 - HMPREF1058_RS06680 0.17 -1.6 420,090 + HMPREF1058_RS06680 0.17 +0.5 420,153 + HMPREF1058_RS06680 0.20 +0.4 420,194 + HMPREF1058_RS06680 0.22 -1.5 420,198 + HMPREF1058_RS06680 0.22 +0.0 420,227 + HMPREF1058_RS06680 0.24 +0.4 420,227 + HMPREF1058_RS06680 0.24 -1.3 420,231 + HMPREF1058_RS06680 0.24 +0.5 420,231 + +0.4 420,290 + -0.5 420,291 - HMPREF1058_RS06680 0.27 -0.4 420,291 - HMPREF1058_RS06680 0.27 +0.8 420,292 + HMPREF1058_RS06680 0.27 +1.2 420,293 - HMPREF1058_RS06680 0.27 -3.1 420,306 + HMPREF1058_RS06680 0.28 +1.0 420,399 + HMPREF1058_RS06680 0.32 +1.1 420,430 - HMPREF1058_RS06680 0.33 -2.9 420,454 - HMPREF1058_RS06680 0.34 -0.5 420,454 - HMPREF1058_RS06680 0.34 -1.5 420,587 + HMPREF1058_RS06680 0.41 +0.4 420,587 + HMPREF1058_RS06680 0.41 -1.6 420,588 - HMPREF1058_RS06680 0.41 -1.1 420,588 - HMPREF1058_RS06680 0.41 -1.2 420,588 - HMPREF1058_RS06680 0.41 -1.1 420,588 - HMPREF1058_RS06680 0.41 -1.2 420,590 - HMPREF1058_RS06680 0.41 -0.2 420,687 + HMPREF1058_RS06680 0.45 -2.0 420,690 - HMPREF1058_RS06680 0.45 -2.7 420,713 + HMPREF1058_RS06680 0.46 -2.7 420,771 + -0.7 420,771 + HMPREF1058_RS06680 0.49 -3.2 420,772 - HMPREF1058_RS06680 0.49 +2.0 420,843 + HMPREF1058_RS06680 0.53 +0.9 420,843 + HMPREF1058_RS06680 0.53 +0.8 420,844 - HMPREF1058_RS06680 0.53 +1.8 420,995 + HMPREF1058_RS06680 0.60 +0.7 420,996 - HMPREF1058_RS06680 0.60 -2.8 420,998 - HMPREF1058_RS06680 0.60 -0.4 421,004 - HMPREF1058_RS06680 0.60 +2.1 421,108 + HMPREF1058_RS06680 0.65 -3.1 421,128 + HMPREF1058_RS06680 0.66 +1.7 421,129 - HMPREF1058_RS06680 0.66 +1.7 421,284 - HMPREF1058_RS06680 0.73 +0.6 421,286 - HMPREF1058_RS06680 0.73 +1.1 421,290 + HMPREF1058_RS06680 0.73 -0.8 421,312 + HMPREF1058_RS06680 0.74 +1.7 421,329 - HMPREF1058_RS06680 0.75 +0.5 421,329 - HMPREF1058_RS06680 0.75 -0.4 421,357 - HMPREF1058_RS06680 0.76 -1.7 421,377 + HMPREF1058_RS06680 0.77 +0.3 421,377 + HMPREF1058_RS06680 0.77 +0.4 421,462 + HMPREF1058_RS06680 0.81 +1.5 421,488 + HMPREF1058_RS06680 0.83 -1.4 421,489 - HMPREF1058_RS06680 0.83 -0.4 421,580 - HMPREF1058_RS06680 0.87 -0.5 421,611 + HMPREF1058_RS06680 0.88 +0.6 421,612 - HMPREF1058_RS06680 0.88 -1.1 421,614 + HMPREF1058_RS06680 0.88 -1.9 421,638 - HMPREF1058_RS06680 0.90 -1.6 421,651 - +0.7 421,695 + +1.2 421,695 + +1.0 421,696 - +1.1 421,744 + -1.5 421,745 - -0.0 421,766 + -2.5 421,779 + -1.6 421,793 + +2.6 421,793 + -1.0 421,794 - +3.9 421,794 - +0.5 421,794 - +0.7 421,803 + -0.4 421,804 - -0.2 421,829 - +1.3 421,829 - -1.1 421,956 - HMPREF1058_RS06675 0.10 +1.0 422,012 - HMPREF1058_RS06675 0.17 +0.9 422,012 - HMPREF1058_RS06675 0.17 -2.3 422,021 + HMPREF1058_RS06675 0.18 -1.5 422,062 + HMPREF1058_RS06675 0.22 -0.3 422,089 + HMPREF1058_RS06675 0.26 +1.0 422,102 - HMPREF1058_RS06675 0.27 +0.4 422,134 + HMPREF1058_RS06675 0.31 -0.7 422,137 - HMPREF1058_RS06675 0.31 -2.3 422,138 + HMPREF1058_RS06675 0.31 +1.4 422,138 + HMPREF1058_RS06675 0.31 -1.1 422,138 + HMPREF1058_RS06675 0.31 -0.9 422,138 + HMPREF1058_RS06675 0.31 -2.3 422,146 + HMPREF1058_RS06675 0.32 +1.3 422,196 + HMPREF1058_RS06675 0.38 +0.4 422,196 + HMPREF1058_RS06675 0.38 -0.4 422,197 - HMPREF1058_RS06675 0.38 -0.2 422,197 - HMPREF1058_RS06675 0.38 -0.7 422,198 + HMPREF1058_RS06675 0.38 -0.8 422,214 - HMPREF1058_RS06675 0.40 -0.2 422,229 + HMPREF1058_RS06675 0.42 +2.6 422,229 + HMPREF1058_RS06675 0.42 -0.5 422,363 + HMPREF1058_RS06675 0.57 -0.9 422,364 - HMPREF1058_RS06675 0.57 -0.3 422,367 + HMPREF1058_RS06675 0.58 +0.6 422,368 - HMPREF1058_RS06675 0.58 -1.3 422,368 - HMPREF1058_RS06675 0.58 -0.5 422,368 - HMPREF1058_RS06675 0.58 +0.8 422,452 + HMPREF1058_RS06675 0.68 -1.8 422,486 + HMPREF1058_RS06675 0.72 -1.3 422,499 - HMPREF1058_RS06675 0.73 +0.7 422,516 + HMPREF1058_RS06675 0.75 +0.8 422,517 - HMPREF1058_RS06675 0.75 -0.4 422,564 + HMPREF1058_RS06675 0.81 -0.9 422,603 - HMPREF1058_RS06675 0.85 +0.6 422,613 - HMPREF1058_RS06675 0.86 -1.6 422,615 - HMPREF1058_RS06675 0.86 +3.2 422,698 - +1.0 422,736 + +2.6 422,737 - -0.9
Or see this region's nucleotide sequence