Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06620 and HMPREF1058_RS06615 are separated by 175 nucleotides HMPREF1058_RS06615 and HMPREF1058_RS06610 overlap by 1 nucleotides
HMPREF1058_RS06620: HMPREF1058_RS06620 - mannose-1-phosphate guanylyltransferase, at 435,545 to 436,609
_RS06620
HMPREF1058_RS06615: HMPREF1058_RS06615 - GDP-mannose 4,6-dehydratase, at 436,785 to 437,867
_RS06615
HMPREF1058_RS06610: HMPREF1058_RS06610 - GDP-L-fucose synthase, at 437,867 to 438,943
_RS06610
Position (kb) 436
437
438 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 436.607 kb on + strand at 436.611 kb on + strand at 436.642 kb on + strand at 436.643 kb on - strand at 436.643 kb on - strand at 436.653 kb on + strand at 436.654 kb on - strand at 436.654 kb on - strand at 436.717 kb on - strand at 436.717 kb on - strand at 436.723 kb on - strand at 436.726 kb on + strand at 436.730 kb on + strand at 436.730 kb on + strand at 436.731 kb on - strand at 436.731 kb on - strand at 436.731 kb on - strand at 436.773 kb on - strand at 436.812 kb on - strand at 436.812 kb on - strand at 436.927 kb on - strand, within HMPREF1058_RS06615 at 437.016 kb on - strand, within HMPREF1058_RS06615 at 437.048 kb on + strand, within HMPREF1058_RS06615 at 437.133 kb on - strand, within HMPREF1058_RS06615 at 437.151 kb on - strand at 437.324 kb on + strand, within HMPREF1058_RS06615 at 437.493 kb on + strand, within HMPREF1058_RS06615 at 437.504 kb on + strand, within HMPREF1058_RS06615 at 437.505 kb on - strand, within HMPREF1058_RS06615 at 437.562 kb on + strand, within HMPREF1058_RS06615 at 437.563 kb on - strand, within HMPREF1058_RS06615 at 437.586 kb on - strand, within HMPREF1058_RS06615 at 437.697 kb on + strand, within HMPREF1058_RS06615 at 437.819 kb on + strand at 437.865 kb on + strand at 437.886 kb on + strand at 437.886 kb on + strand at 437.887 kb on - strand at 437.889 kb on - strand at 437.889 kb on - strand at 437.923 kb on + strand at 437.954 kb on - strand at 437.954 kb on - strand at 438.042 kb on - strand, within HMPREF1058_RS06610 at 438.166 kb on - strand, within HMPREF1058_RS06610 at 438.260 kb on + strand, within HMPREF1058_RS06610 at 438.261 kb on - strand, within HMPREF1058_RS06610 at 438.263 kb on - strand, within HMPREF1058_RS06610 at 438.275 kb on + strand, within HMPREF1058_RS06610 at 438.342 kb on - strand, within HMPREF1058_RS06610 at 438.383 kb on - strand, within HMPREF1058_RS06610 at 438.484 kb on + strand, within HMPREF1058_RS06610 at 438.579 kb on + strand, within HMPREF1058_RS06610 at 438.650 kb on + strand, within HMPREF1058_RS06610 at 438.651 kb on - strand, within HMPREF1058_RS06610 at 438.651 kb on - strand, within HMPREF1058_RS06610 at 438.683 kb on + strand, within HMPREF1058_RS06610 at 438.684 kb on - strand, within HMPREF1058_RS06610 at 438.838 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 436,607 + -0.4 436,611 + +1.9 436,642 + +2.6 436,643 - +1.4 436,643 - -2.4 436,653 + +0.4 436,654 - -0.2 436,654 - +1.9 436,717 - -3.3 436,717 - -1.2 436,723 - -1.1 436,726 + -2.6 436,730 + +0.6 436,730 + -0.3 436,731 - -2.6 436,731 - +1.2 436,731 - -0.2 436,773 - -3.3 436,812 - +1.1 436,812 - +2.3 436,927 - HMPREF1058_RS06615 0.13 +3.5 437,016 - HMPREF1058_RS06615 0.21 -1.4 437,048 + HMPREF1058_RS06615 0.24 +0.3 437,133 - HMPREF1058_RS06615 0.32 -1.0 437,151 - +2.2 437,324 + HMPREF1058_RS06615 0.50 -2.8 437,493 + HMPREF1058_RS06615 0.65 -1.7 437,504 + HMPREF1058_RS06615 0.66 -1.1 437,505 - HMPREF1058_RS06615 0.66 +0.4 437,562 + HMPREF1058_RS06615 0.72 +0.2 437,563 - HMPREF1058_RS06615 0.72 -0.8 437,586 - HMPREF1058_RS06615 0.74 +2.7 437,697 + HMPREF1058_RS06615 0.84 -2.4 437,819 + -2.9 437,865 + -1.1 437,886 + -1.2 437,886 + +3.5 437,887 - -0.3 437,889 - -1.1 437,889 - -1.4 437,923 + -1.3 437,954 - +1.5 437,954 - +0.6 438,042 - HMPREF1058_RS06610 0.16 -2.5 438,166 - HMPREF1058_RS06610 0.28 +1.2 438,260 + HMPREF1058_RS06610 0.36 +1.7 438,261 - HMPREF1058_RS06610 0.37 +0.3 438,263 - HMPREF1058_RS06610 0.37 -0.4 438,275 + HMPREF1058_RS06610 0.38 +1.9 438,342 - HMPREF1058_RS06610 0.44 -2.3 438,383 - HMPREF1058_RS06610 0.48 +0.6 438,484 + HMPREF1058_RS06610 0.57 +0.6 438,579 + HMPREF1058_RS06610 0.66 +2.0 438,650 + HMPREF1058_RS06610 0.73 -0.0 438,651 - HMPREF1058_RS06610 0.73 +0.0 438,651 - HMPREF1058_RS06610 0.73 -0.2 438,683 + HMPREF1058_RS06610 0.76 +1.0 438,684 - HMPREF1058_RS06610 0.76 -2.9 438,838 + -0.5
Or see this region's nucleotide sequence