Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05770 and HMPREF1058_RS05765 are separated by 193 nucleotides HMPREF1058_RS05765 and HMPREF1058_RS05760 are separated by 209 nucleotides HMPREF1058_RS05760 and HMPREF1058_RS05755 are separated by 13 nucleotides
HMPREF1058_RS05770: HMPREF1058_RS05770 - flotillin-like protein FloA, at 83,375 to 84,373
_RS05770
HMPREF1058_RS05765: HMPREF1058_RS05765 - nucleoside phosphorylase, at 84,567 to 85,445
_RS05765
HMPREF1058_RS05760: HMPREF1058_RS05760 - GAF domain-containing protein, at 85,655 to 86,140
_RS05760
HMPREF1058_RS05755: HMPREF1058_RS05755 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 86,154 to 87,647
_RS05755
Position (kb) 84
85
86 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 83.622 kb on - strand, within HMPREF1058_RS05770 at 83.622 kb on - strand, within HMPREF1058_RS05770 at 83.622 kb on - strand at 83.622 kb on - strand, within HMPREF1058_RS05770 at 83.624 kb on - strand, within HMPREF1058_RS05770 at 83.662 kb on - strand, within HMPREF1058_RS05770 at 83.662 kb on - strand, within HMPREF1058_RS05770 at 83.737 kb on - strand, within HMPREF1058_RS05770 at 83.767 kb on + strand, within HMPREF1058_RS05770 at 83.767 kb on + strand, within HMPREF1058_RS05770 at 83.814 kb on + strand, within HMPREF1058_RS05770 at 83.915 kb on - strand at 83.915 kb on - strand, within HMPREF1058_RS05770 at 83.974 kb on + strand, within HMPREF1058_RS05770 at 83.980 kb on + strand, within HMPREF1058_RS05770 at 83.985 kb on + strand, within HMPREF1058_RS05770 at 84.004 kb on - strand, within HMPREF1058_RS05770 at 84.040 kb on + strand, within HMPREF1058_RS05770 at 84.041 kb on - strand, within HMPREF1058_RS05770 at 84.042 kb on + strand, within HMPREF1058_RS05770 at 84.113 kb on - strand, within HMPREF1058_RS05770 at 84.205 kb on - strand, within HMPREF1058_RS05770 at 84.265 kb on - strand, within HMPREF1058_RS05770 at 84.278 kb on + strand at 84.298 kb on - strand at 84.307 kb on + strand at 84.307 kb on + strand at 84.325 kb on + strand at 84.363 kb on + strand at 84.364 kb on - strand at 84.371 kb on + strand at 84.377 kb on + strand at 84.380 kb on + strand at 84.392 kb on - strand at 84.441 kb on + strand at 84.445 kb on - strand at 84.475 kb on + strand at 84.476 kb on - strand at 84.532 kb on - strand at 84.566 kb on + strand at 84.579 kb on + strand at 84.618 kb on - strand at 84.667 kb on + strand, within HMPREF1058_RS05765 at 84.668 kb on - strand, within HMPREF1058_RS05765 at 84.689 kb on - strand, within HMPREF1058_RS05765 at 84.694 kb on + strand, within HMPREF1058_RS05765 at 84.697 kb on + strand, within HMPREF1058_RS05765 at 84.697 kb on + strand, within HMPREF1058_RS05765 at 84.697 kb on + strand, within HMPREF1058_RS05765 at 84.731 kb on - strand, within HMPREF1058_RS05765 at 84.731 kb on - strand, within HMPREF1058_RS05765 at 84.731 kb on - strand, within HMPREF1058_RS05765 at 84.765 kb on + strand, within HMPREF1058_RS05765 at 84.765 kb on + strand, within HMPREF1058_RS05765 at 84.765 kb on + strand, within HMPREF1058_RS05765 at 84.766 kb on - strand, within HMPREF1058_RS05765 at 84.766 kb on - strand, within HMPREF1058_RS05765 at 84.768 kb on - strand, within HMPREF1058_RS05765 at 84.768 kb on - strand, within HMPREF1058_RS05765 at 84.787 kb on + strand, within HMPREF1058_RS05765 at 84.788 kb on - strand, within HMPREF1058_RS05765 at 84.802 kb on + strand, within HMPREF1058_RS05765 at 84.802 kb on + strand, within HMPREF1058_RS05765 at 84.815 kb on + strand, within HMPREF1058_RS05765 at 84.815 kb on + strand, within HMPREF1058_RS05765 at 84.815 kb on + strand, within HMPREF1058_RS05765 at 84.816 kb on - strand, within HMPREF1058_RS05765 at 84.818 kb on - strand, within HMPREF1058_RS05765 at 84.942 kb on - strand at 84.957 kb on + strand, within HMPREF1058_RS05765 at 84.958 kb on - strand, within HMPREF1058_RS05765 at 85.060 kb on - strand, within HMPREF1058_RS05765 at 85.076 kb on + strand, within HMPREF1058_RS05765 at 85.081 kb on + strand, within HMPREF1058_RS05765 at 85.081 kb on + strand, within HMPREF1058_RS05765 at 85.081 kb on + strand, within HMPREF1058_RS05765 at 85.081 kb on + strand, within HMPREF1058_RS05765 at 85.081 kb on + strand, within HMPREF1058_RS05765 at 85.082 kb on - strand, within HMPREF1058_RS05765 at 85.099 kb on - strand, within HMPREF1058_RS05765 at 85.162 kb on + strand, within HMPREF1058_RS05765 at 85.349 kb on - strand, within HMPREF1058_RS05765 at 85.349 kb on - strand, within HMPREF1058_RS05765 at 85.353 kb on - strand, within HMPREF1058_RS05765 at 85.444 kb on - strand at 85.444 kb on - strand at 85.455 kb on + strand at 85.463 kb on + strand at 85.467 kb on + strand at 85.468 kb on - strand at 85.468 kb on - strand at 85.468 kb on - strand at 85.596 kb on + strand at 85.639 kb on + strand at 85.649 kb on - strand at 85.685 kb on - strand at 85.693 kb on - strand at 85.731 kb on + strand, within HMPREF1058_RS05760 at 85.843 kb on + strand, within HMPREF1058_RS05760 at 85.902 kb on + strand, within HMPREF1058_RS05760 at 85.903 kb on - strand at 85.950 kb on + strand, within HMPREF1058_RS05760 at 85.969 kb on + strand, within HMPREF1058_RS05760 at 85.970 kb on - strand, within HMPREF1058_RS05760 at 86.011 kb on + strand, within HMPREF1058_RS05760 at 86.031 kb on + strand, within HMPREF1058_RS05760 at 86.057 kb on - strand, within HMPREF1058_RS05760 at 86.119 kb on - strand at 86.122 kb on + strand at 86.219 kb on - strand at 86.298 kb on + strand at 86.299 kb on - strand at 86.299 kb on - strand at 86.300 kb on + strand at 86.301 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 83,622 - HMPREF1058_RS05770 0.25 -1.3 83,622 - HMPREF1058_RS05770 0.25 -0.9 83,622 - +0.3 83,622 - HMPREF1058_RS05770 0.25 -1.6 83,624 - HMPREF1058_RS05770 0.25 -0.7 83,662 - HMPREF1058_RS05770 0.29 -1.1 83,662 - HMPREF1058_RS05770 0.29 -1.5 83,737 - HMPREF1058_RS05770 0.36 -1.1 83,767 + HMPREF1058_RS05770 0.39 +0.2 83,767 + HMPREF1058_RS05770 0.39 +1.5 83,814 + HMPREF1058_RS05770 0.44 +1.7 83,915 - -1.0 83,915 - HMPREF1058_RS05770 0.54 -0.6 83,974 + HMPREF1058_RS05770 0.60 +0.6 83,980 + HMPREF1058_RS05770 0.61 +0.9 83,985 + HMPREF1058_RS05770 0.61 +1.6 84,004 - HMPREF1058_RS05770 0.63 -3.6 84,040 + HMPREF1058_RS05770 0.67 +0.7 84,041 - HMPREF1058_RS05770 0.67 -2.7 84,042 + HMPREF1058_RS05770 0.67 +0.6 84,113 - HMPREF1058_RS05770 0.74 -3.4 84,205 - HMPREF1058_RS05770 0.83 -2.9 84,265 - HMPREF1058_RS05770 0.89 +0.3 84,278 + -0.2 84,298 - -2.8 84,307 + +0.1 84,307 + +2.2 84,325 + -0.9 84,363 + -1.9 84,364 - -4.5 84,371 + +0.5 84,377 + +0.4 84,380 + -0.0 84,392 - -0.7 84,441 + +0.6 84,445 - -0.4 84,475 + +1.0 84,476 - -3.0 84,532 - -1.6 84,566 + -2.3 84,579 + -1.0 84,618 - -1.7 84,667 + HMPREF1058_RS05765 0.11 -3.2 84,668 - HMPREF1058_RS05765 0.11 -0.9 84,689 - HMPREF1058_RS05765 0.14 -3.1 84,694 + HMPREF1058_RS05765 0.14 -3.3 84,697 + HMPREF1058_RS05765 0.15 -3.7 84,697 + HMPREF1058_RS05765 0.15 -0.4 84,697 + HMPREF1058_RS05765 0.15 -2.5 84,731 - HMPREF1058_RS05765 0.19 -4.5 84,731 - HMPREF1058_RS05765 0.19 -1.6 84,731 - HMPREF1058_RS05765 0.19 -1.8 84,765 + HMPREF1058_RS05765 0.23 -1.0 84,765 + HMPREF1058_RS05765 0.23 -3.2 84,765 + HMPREF1058_RS05765 0.23 -0.7 84,766 - HMPREF1058_RS05765 0.23 -2.8 84,766 - HMPREF1058_RS05765 0.23 -3.1 84,768 - HMPREF1058_RS05765 0.23 -2.1 84,768 - HMPREF1058_RS05765 0.23 -0.4 84,787 + HMPREF1058_RS05765 0.25 -2.6 84,788 - HMPREF1058_RS05765 0.25 -0.9 84,802 + HMPREF1058_RS05765 0.27 -3.2 84,802 + HMPREF1058_RS05765 0.27 -3.3 84,815 + HMPREF1058_RS05765 0.28 -2.8 84,815 + HMPREF1058_RS05765 0.28 -1.9 84,815 + HMPREF1058_RS05765 0.28 -0.0 84,816 - HMPREF1058_RS05765 0.28 -2.6 84,818 - HMPREF1058_RS05765 0.29 -0.9 84,942 - -1.1 84,957 + HMPREF1058_RS05765 0.44 -0.8 84,958 - HMPREF1058_RS05765 0.44 -1.5 85,060 - HMPREF1058_RS05765 0.56 -0.4 85,076 + HMPREF1058_RS05765 0.58 -2.3 85,081 + HMPREF1058_RS05765 0.58 -3.7 85,081 + HMPREF1058_RS05765 0.58 -3.1 85,081 + HMPREF1058_RS05765 0.58 -3.3 85,081 + HMPREF1058_RS05765 0.58 -2.1 85,081 + HMPREF1058_RS05765 0.58 -4.2 85,082 - HMPREF1058_RS05765 0.59 -1.9 85,099 - HMPREF1058_RS05765 0.61 -0.6 85,162 + HMPREF1058_RS05765 0.68 -1.1 85,349 - HMPREF1058_RS05765 0.89 -2.8 85,349 - HMPREF1058_RS05765 0.89 -2.3 85,353 - HMPREF1058_RS05765 0.89 -2.3 85,444 - -2.0 85,444 - -3.3 85,455 + -0.0 85,463 + +0.6 85,467 + -0.9 85,468 - -3.1 85,468 - -2.0 85,468 - -3.1 85,596 + +0.6 85,639 + -0.4 85,649 - -0.7 85,685 - -1.9 85,693 - -0.0 85,731 + HMPREF1058_RS05760 0.16 -0.3 85,843 + HMPREF1058_RS05760 0.39 +0.3 85,902 + HMPREF1058_RS05760 0.51 -1.8 85,903 - -0.9 85,950 + HMPREF1058_RS05760 0.61 -0.1 85,969 + HMPREF1058_RS05760 0.65 +0.4 85,970 - HMPREF1058_RS05760 0.65 -3.8 86,011 + HMPREF1058_RS05760 0.73 -1.3 86,031 + HMPREF1058_RS05760 0.77 +1.5 86,057 - HMPREF1058_RS05760 0.83 -1.1 86,119 - -2.8 86,122 + -0.0 86,219 - -1.4 86,298 + -3.4 86,299 - -2.1 86,299 - -2.9 86,300 + -3.5 86,301 - -2.9
Or see this region's nucleotide sequence