Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05400 and HMPREF1058_RS05395 are separated by 27 nucleotides HMPREF1058_RS05395 and HMPREF1058_RS05390 are separated by 16 nucleotides HMPREF1058_RS05390 and HMPREF1058_RS05385 are separated by 52 nucleotides
HMPREF1058_RS05400: HMPREF1058_RS05400 - cation:proton antiporter, at 172,226 to 174,472
_RS05400
HMPREF1058_RS05395: HMPREF1058_RS05395 - alpha-glucan family phosphorylase, at 174,500 to 177,058
_RS05395
HMPREF1058_RS05390: HMPREF1058_RS05390 - arginase family protein, at 177,075 to 177,884
_RS05390
HMPREF1058_RS05385: HMPREF1058_RS05385 - methylglyoxal synthase, at 177,937 to 178,473
_RS05385
Position (kb) 174
175
176
177
178 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 173.547 kb on - strand, within HMPREF1058_RS05400 at 173.559 kb on + strand, within HMPREF1058_RS05400 at 173.559 kb on + strand, within HMPREF1058_RS05400 at 173.736 kb on + strand, within HMPREF1058_RS05400 at 173.737 kb on - strand, within HMPREF1058_RS05400 at 173.739 kb on + strand, within HMPREF1058_RS05400 at 173.739 kb on + strand, within HMPREF1058_RS05400 at 173.739 kb on + strand, within HMPREF1058_RS05400 at 173.740 kb on - strand, within HMPREF1058_RS05400 at 173.740 kb on - strand, within HMPREF1058_RS05400 at 173.824 kb on - strand, within HMPREF1058_RS05400 at 173.902 kb on + strand, within HMPREF1058_RS05400 at 174.033 kb on - strand, within HMPREF1058_RS05400 at 174.124 kb on - strand, within HMPREF1058_RS05400 at 174.225 kb on + strand, within HMPREF1058_RS05400 at 174.334 kb on - strand at 174.376 kb on + strand at 174.377 kb on - strand at 174.379 kb on + strand at 174.418 kb on + strand at 174.517 kb on + strand at 174.517 kb on + strand at 174.523 kb on - strand at 174.548 kb on - strand at 174.714 kb on - strand at 174.756 kb on - strand, within HMPREF1058_RS05395 at 174.869 kb on - strand, within HMPREF1058_RS05395 at 174.873 kb on + strand, within HMPREF1058_RS05395 at 174.873 kb on + strand, within HMPREF1058_RS05395 at 175.119 kb on + strand, within HMPREF1058_RS05395 at 175.321 kb on - strand, within HMPREF1058_RS05395 at 175.330 kb on + strand, within HMPREF1058_RS05395 at 175.376 kb on + strand, within HMPREF1058_RS05395 at 175.380 kb on + strand, within HMPREF1058_RS05395 at 175.381 kb on - strand, within HMPREF1058_RS05395 at 175.443 kb on + strand, within HMPREF1058_RS05395 at 175.443 kb on + strand, within HMPREF1058_RS05395 at 175.448 kb on - strand, within HMPREF1058_RS05395 at 175.448 kb on - strand at 175.473 kb on - strand, within HMPREF1058_RS05395 at 175.500 kb on - strand, within HMPREF1058_RS05395 at 175.500 kb on - strand, within HMPREF1058_RS05395 at 175.629 kb on + strand, within HMPREF1058_RS05395 at 175.722 kb on - strand, within HMPREF1058_RS05395 at 175.725 kb on + strand, within HMPREF1058_RS05395 at 176.004 kb on + strand, within HMPREF1058_RS05395 at 176.005 kb on - strand, within HMPREF1058_RS05395 at 176.033 kb on + strand, within HMPREF1058_RS05395 at 176.033 kb on + strand, within HMPREF1058_RS05395 at 176.033 kb on + strand, within HMPREF1058_RS05395 at 176.033 kb on + strand, within HMPREF1058_RS05395 at 176.034 kb on - strand, within HMPREF1058_RS05395 at 176.034 kb on - strand, within HMPREF1058_RS05395 at 176.035 kb on + strand, within HMPREF1058_RS05395 at 176.036 kb on - strand, within HMPREF1058_RS05395 at 176.064 kb on + strand, within HMPREF1058_RS05395 at 176.065 kb on - strand, within HMPREF1058_RS05395 at 176.100 kb on - strand, within HMPREF1058_RS05395 at 176.229 kb on - strand, within HMPREF1058_RS05395 at 176.347 kb on + strand, within HMPREF1058_RS05395 at 176.422 kb on + strand, within HMPREF1058_RS05395 at 176.423 kb on - strand, within HMPREF1058_RS05395 at 176.423 kb on - strand, within HMPREF1058_RS05395 at 176.449 kb on + strand, within HMPREF1058_RS05395 at 176.505 kb on - strand, within HMPREF1058_RS05395 at 176.549 kb on + strand, within HMPREF1058_RS05395 at 176.550 kb on - strand, within HMPREF1058_RS05395 at 176.550 kb on - strand, within HMPREF1058_RS05395 at 176.615 kb on + strand, within HMPREF1058_RS05395 at 176.616 kb on - strand, within HMPREF1058_RS05395 at 176.889 kb on + strand at 176.889 kb on + strand at 176.890 kb on - strand at 176.890 kb on - strand at 176.890 kb on - strand at 176.890 kb on - strand at 176.890 kb on - strand at 176.892 kb on - strand at 176.934 kb on + strand at 176.939 kb on - strand at 177.042 kb on - strand at 177.046 kb on - strand at 177.046 kb on - strand at 177.046 kb on - strand at 177.046 kb on - strand at 177.090 kb on - strand at 177.090 kb on - strand at 177.162 kb on + strand, within HMPREF1058_RS05390 at 177.163 kb on - strand, within HMPREF1058_RS05390 at 177.197 kb on + strand, within HMPREF1058_RS05390 at 177.197 kb on + strand, within HMPREF1058_RS05390 at 177.198 kb on - strand, within HMPREF1058_RS05390 at 177.198 kb on - strand, within HMPREF1058_RS05390 at 177.223 kb on - strand, within HMPREF1058_RS05390 at 177.228 kb on - strand, within HMPREF1058_RS05390 at 177.254 kb on + strand, within HMPREF1058_RS05390 at 177.456 kb on - strand, within HMPREF1058_RS05390 at 177.456 kb on - strand, within HMPREF1058_RS05390 at 177.458 kb on - strand, within HMPREF1058_RS05390 at 177.467 kb on + strand, within HMPREF1058_RS05390 at 177.488 kb on - strand, within HMPREF1058_RS05390 at 177.497 kb on - strand, within HMPREF1058_RS05390 at 177.517 kb on + strand, within HMPREF1058_RS05390 at 177.569 kb on + strand, within HMPREF1058_RS05390 at 177.648 kb on + strand, within HMPREF1058_RS05390 at 177.731 kb on + strand, within HMPREF1058_RS05390 at 177.999 kb on - strand, within HMPREF1058_RS05385 at 178.051 kb on - strand, within HMPREF1058_RS05385
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 173,547 - HMPREF1058_RS05400 0.59 -1.4 173,559 + HMPREF1058_RS05400 0.59 -1.1 173,559 + HMPREF1058_RS05400 0.59 +0.2 173,736 + HMPREF1058_RS05400 0.67 -0.9 173,737 - HMPREF1058_RS05400 0.67 -1.0 173,739 + HMPREF1058_RS05400 0.67 -2.8 173,739 + HMPREF1058_RS05400 0.67 -0.3 173,739 + HMPREF1058_RS05400 0.67 -0.4 173,740 - HMPREF1058_RS05400 0.67 +0.1 173,740 - HMPREF1058_RS05400 0.67 -0.1 173,824 - HMPREF1058_RS05400 0.71 -1.3 173,902 + HMPREF1058_RS05400 0.75 -1.5 174,033 - HMPREF1058_RS05400 0.80 -2.1 174,124 - HMPREF1058_RS05400 0.84 -0.9 174,225 + HMPREF1058_RS05400 0.89 -2.5 174,334 - -1.4 174,376 + +0.2 174,377 - +1.1 174,379 + -1.9 174,418 + -3.6 174,517 + +1.4 174,517 + +0.1 174,523 - -0.0 174,548 - +1.0 174,714 - -0.0 174,756 - HMPREF1058_RS05395 0.10 -0.4 174,869 - HMPREF1058_RS05395 0.14 +2.6 174,873 + HMPREF1058_RS05395 0.15 -0.7 174,873 + HMPREF1058_RS05395 0.15 -1.3 175,119 + HMPREF1058_RS05395 0.24 -2.2 175,321 - HMPREF1058_RS05395 0.32 +2.3 175,330 + HMPREF1058_RS05395 0.32 +0.7 175,376 + HMPREF1058_RS05395 0.34 +0.3 175,380 + HMPREF1058_RS05395 0.34 -0.9 175,381 - HMPREF1058_RS05395 0.34 -0.3 175,443 + HMPREF1058_RS05395 0.37 +2.1 175,443 + HMPREF1058_RS05395 0.37 +0.2 175,448 - HMPREF1058_RS05395 0.37 -0.0 175,448 - +1.0 175,473 - HMPREF1058_RS05395 0.38 +0.2 175,500 - HMPREF1058_RS05395 0.39 +1.8 175,500 - HMPREF1058_RS05395 0.39 -2.1 175,629 + HMPREF1058_RS05395 0.44 +1.8 175,722 - HMPREF1058_RS05395 0.48 +2.9 175,725 + HMPREF1058_RS05395 0.48 +1.3 176,004 + HMPREF1058_RS05395 0.59 -1.6 176,005 - HMPREF1058_RS05395 0.59 +0.2 176,033 + HMPREF1058_RS05395 0.60 +2.8 176,033 + HMPREF1058_RS05395 0.60 -3.1 176,033 + HMPREF1058_RS05395 0.60 -2.5 176,033 + HMPREF1058_RS05395 0.60 -1.8 176,034 - HMPREF1058_RS05395 0.60 -2.3 176,034 - HMPREF1058_RS05395 0.60 -0.2 176,035 + HMPREF1058_RS05395 0.60 -1.8 176,036 - HMPREF1058_RS05395 0.60 +0.2 176,064 + HMPREF1058_RS05395 0.61 -1.9 176,065 - HMPREF1058_RS05395 0.61 -0.7 176,100 - HMPREF1058_RS05395 0.63 -0.7 176,229 - HMPREF1058_RS05395 0.68 +0.2 176,347 + HMPREF1058_RS05395 0.72 -0.0 176,422 + HMPREF1058_RS05395 0.75 -1.0 176,423 - HMPREF1058_RS05395 0.75 +2.0 176,423 - HMPREF1058_RS05395 0.75 -1.6 176,449 + HMPREF1058_RS05395 0.76 +0.6 176,505 - HMPREF1058_RS05395 0.78 -1.1 176,549 + HMPREF1058_RS05395 0.80 +1.0 176,550 - HMPREF1058_RS05395 0.80 -1.1 176,550 - HMPREF1058_RS05395 0.80 -1.1 176,615 + HMPREF1058_RS05395 0.83 +0.6 176,616 - HMPREF1058_RS05395 0.83 -0.0 176,889 + +1.8 176,889 + -2.8 176,890 - +1.6 176,890 - -1.3 176,890 - -3.6 176,890 - -1.2 176,890 - -0.4 176,892 - +0.4 176,934 + -1.2 176,939 - +0.0 177,042 - +0.6 177,046 - -0.6 177,046 - +0.3 177,046 - -1.8 177,046 - +1.3 177,090 - -2.8 177,090 - +1.0 177,162 + HMPREF1058_RS05390 0.11 -1.8 177,163 - HMPREF1058_RS05390 0.11 -0.4 177,197 + HMPREF1058_RS05390 0.15 -1.0 177,197 + HMPREF1058_RS05390 0.15 +1.2 177,198 - HMPREF1058_RS05390 0.15 -1.3 177,198 - HMPREF1058_RS05390 0.15 -1.0 177,223 - HMPREF1058_RS05390 0.18 -0.9 177,228 - HMPREF1058_RS05390 0.19 +1.6 177,254 + HMPREF1058_RS05390 0.22 -2.1 177,456 - HMPREF1058_RS05390 0.47 +0.2 177,456 - HMPREF1058_RS05390 0.47 +0.6 177,458 - HMPREF1058_RS05390 0.47 +2.1 177,467 + HMPREF1058_RS05390 0.48 +2.6 177,488 - HMPREF1058_RS05390 0.51 +0.8 177,497 - HMPREF1058_RS05390 0.52 -0.6 177,517 + HMPREF1058_RS05390 0.55 -0.7 177,569 + HMPREF1058_RS05390 0.61 +0.1 177,648 + HMPREF1058_RS05390 0.71 -0.3 177,731 + HMPREF1058_RS05390 0.81 -1.4 177,999 - HMPREF1058_RS05385 0.12 -0.3 178,051 - HMPREF1058_RS05385 0.21 +0.3
Or see this region's nucleotide sequence