Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS03330 and HMPREF1058_RS03325 are separated by 123 nucleotides HMPREF1058_RS03325 and HMPREF1058_RS03320 are separated by 12 nucleotides HMPREF1058_RS03320 and HMPREF1058_RS03315 are separated by 47 nucleotides HMPREF1058_RS03315 and HMPREF1058_RS03310 are separated by 173 nucleotides HMPREF1058_RS03310 and HMPREF1058_RS03305 are separated by 32 nucleotides
HMPREF1058_RS03330: HMPREF1058_RS03330 - glutamine synthetase, at 306,838 to 308,340
_RS03330
HMPREF1058_RS03325: HMPREF1058_RS03325 - tRNA-Gly, at 308,464 to 308,536
_RS03325
HMPREF1058_RS03320: HMPREF1058_RS03320 - tRNA-Leu, at 308,549 to 308,632
_RS03320
HMPREF1058_RS03315: HMPREF1058_RS03315 - tRNA-Gly, at 308,680 to 308,752
_RS03315
HMPREF1058_RS03310: HMPREF1058_RS03310 - carboxynorspermidine decarboxylase, at 308,926 to 310,089
_RS03310
HMPREF1058_RS03305: HMPREF1058_RS03305 - UvrD-helicase domain-containing protein, at 310,122 to 312,512
_RS03305
Position (kb) 308
309
310
311 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 308.016 kb on - strand, within HMPREF1058_RS03330 at 308.202 kb on + strand at 308.254 kb on + strand at 308.323 kb on + strand at 308.366 kb on + strand at 308.374 kb on + strand at 308.420 kb on - strand at 308.439 kb on - strand at 308.528 kb on + strand at 308.670 kb on + strand at 308.872 kb on - strand at 308.872 kb on - strand at 308.922 kb on - strand at 308.934 kb on + strand at 308.971 kb on + strand at 308.972 kb on - strand at 308.982 kb on + strand at 308.985 kb on + strand at 308.986 kb on - strand at 308.991 kb on + strand at 309.006 kb on + strand at 309.063 kb on + strand, within HMPREF1058_RS03310 at 309.063 kb on + strand, within HMPREF1058_RS03310 at 309.064 kb on - strand, within HMPREF1058_RS03310 at 309.064 kb on - strand, within HMPREF1058_RS03310 at 309.088 kb on + strand, within HMPREF1058_RS03310 at 309.125 kb on + strand, within HMPREF1058_RS03310 at 309.126 kb on - strand, within HMPREF1058_RS03310 at 309.316 kb on + strand, within HMPREF1058_RS03310 at 309.319 kb on - strand, within HMPREF1058_RS03310 at 309.319 kb on - strand, within HMPREF1058_RS03310 at 309.526 kb on + strand, within HMPREF1058_RS03310 at 309.527 kb on - strand, within HMPREF1058_RS03310 at 309.527 kb on - strand, within HMPREF1058_RS03310 at 309.541 kb on - strand, within HMPREF1058_RS03310 at 309.747 kb on + strand, within HMPREF1058_RS03310 at 309.752 kb on + strand, within HMPREF1058_RS03310 at 309.780 kb on + strand, within HMPREF1058_RS03310 at 309.787 kb on + strand, within HMPREF1058_RS03310 at 309.788 kb on - strand, within HMPREF1058_RS03310 at 309.789 kb on + strand, within HMPREF1058_RS03310 at 309.845 kb on + strand, within HMPREF1058_RS03310 at 309.846 kb on - strand, within HMPREF1058_RS03310 at 309.893 kb on + strand, within HMPREF1058_RS03310 at 309.912 kb on + strand, within HMPREF1058_RS03310 at 309.912 kb on + strand, within HMPREF1058_RS03310 at 309.913 kb on - strand, within HMPREF1058_RS03310 at 310.052 kb on - strand at 310.052 kb on - strand at 310.081 kb on - strand at 310.354 kb on - strand at 310.356 kb on - strand at 310.368 kb on - strand, within HMPREF1058_RS03305 at 310.581 kb on - strand, within HMPREF1058_RS03305 at 310.581 kb on - strand, within HMPREF1058_RS03305 at 310.629 kb on - strand, within HMPREF1058_RS03305 at 310.655 kb on - strand, within HMPREF1058_RS03305
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 308,016 - HMPREF1058_RS03330 0.78 +0.9 308,202 + -2.8 308,254 + -1.1 308,323 + -2.0 308,366 + -1.6 308,374 + -2.6 308,420 - +1.2 308,439 - +1.7 308,528 + -0.0 308,670 + +0.4 308,872 - -1.9 308,872 - +1.9 308,922 - +0.1 308,934 + -2.4 308,971 + -0.9 308,972 - -4.9 308,982 + -4.3 308,985 + -2.8 308,986 - -5.2 308,991 + -4.7 309,006 + -3.1 309,063 + HMPREF1058_RS03310 0.12 -1.5 309,063 + HMPREF1058_RS03310 0.12 -5.1 309,064 - HMPREF1058_RS03310 0.12 -2.3 309,064 - HMPREF1058_RS03310 0.12 -1.5 309,088 + HMPREF1058_RS03310 0.14 -3.1 309,125 + HMPREF1058_RS03310 0.17 -1.3 309,126 - HMPREF1058_RS03310 0.17 -4.1 309,316 + HMPREF1058_RS03310 0.34 -2.5 309,319 - HMPREF1058_RS03310 0.34 -4.7 309,319 - HMPREF1058_RS03310 0.34 -1.9 309,526 + HMPREF1058_RS03310 0.52 -4.3 309,527 - HMPREF1058_RS03310 0.52 -4.2 309,527 - HMPREF1058_RS03310 0.52 -2.3 309,541 - HMPREF1058_RS03310 0.53 -4.3 309,747 + HMPREF1058_RS03310 0.71 -0.7 309,752 + HMPREF1058_RS03310 0.71 -4.6 309,780 + HMPREF1058_RS03310 0.73 -2.4 309,787 + HMPREF1058_RS03310 0.74 -3.6 309,788 - HMPREF1058_RS03310 0.74 -3.6 309,789 + HMPREF1058_RS03310 0.74 -2.5 309,845 + HMPREF1058_RS03310 0.79 -3.1 309,846 - HMPREF1058_RS03310 0.79 -0.9 309,893 + HMPREF1058_RS03310 0.83 -0.2 309,912 + HMPREF1058_RS03310 0.85 -1.8 309,912 + HMPREF1058_RS03310 0.85 -2.6 309,913 - HMPREF1058_RS03310 0.85 -2.5 310,052 - -3.4 310,052 - -2.1 310,081 - -2.8 310,354 - -1.6 310,356 - -1.1 310,368 - HMPREF1058_RS03305 0.10 -0.0 310,581 - HMPREF1058_RS03305 0.19 -1.7 310,581 - HMPREF1058_RS03305 0.19 -0.5 310,629 - HMPREF1058_RS03305 0.21 -0.6 310,655 - HMPREF1058_RS03305 0.22 -2.1
Or see this region's nucleotide sequence