Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS03295 and HMPREF1058_RS03290 overlap by 11 nucleotides HMPREF1058_RS03290 and HMPREF1058_RS03285 are separated by 17 nucleotides HMPREF1058_RS03285 and HMPREF1058_RS03280 are separated by 8 nucleotides
HMPREF1058_RS03295: HMPREF1058_RS03295 - sulfur carrier protein ThiS, at 313,624 to 313,824
_RS03295
HMPREF1058_RS03290: HMPREF1058_RS03290 - thiamine phosphate synthase, at 313,814 to 314,440
_RS03290
HMPREF1058_RS03285: HMPREF1058_RS03285 - thiazole synthase, at 314,458 to 315,231
_RS03285
HMPREF1058_RS03280: HMPREF1058_RS03280 - phosphomethylpyrimidine synthase ThiC, at 315,240 to 316,940
_RS03280
Position (kb) 314
315
316 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 313.461 kb on + strand at 313.462 kb on - strand at 313.505 kb on + strand at 313.507 kb on + strand at 313.507 kb on + strand at 313.550 kb on + strand at 313.618 kb on + strand at 313.619 kb on - strand at 313.620 kb on + strand at 313.621 kb on - strand at 313.621 kb on - strand at 313.623 kb on + strand at 313.659 kb on - strand, within HMPREF1058_RS03295 at 313.754 kb on + strand, within HMPREF1058_RS03295 at 313.865 kb on + strand at 313.866 kb on - strand at 313.866 kb on - strand at 313.866 kb on - strand at 313.871 kb on + strand at 313.875 kb on - strand at 313.924 kb on - strand, within HMPREF1058_RS03290 at 314.106 kb on + strand, within HMPREF1058_RS03290 at 314.107 kb on - strand, within HMPREF1058_RS03290 at 314.155 kb on - strand, within HMPREF1058_RS03290 at 314.183 kb on + strand, within HMPREF1058_RS03290 at 314.184 kb on - strand, within HMPREF1058_RS03290 at 314.388 kb on + strand at 314.389 kb on - strand at 314.441 kb on + strand at 314.455 kb on - strand at 314.455 kb on - strand at 314.475 kb on - strand at 314.490 kb on + strand at 314.623 kb on - strand, within HMPREF1058_RS03285 at 314.634 kb on + strand, within HMPREF1058_RS03285 at 314.651 kb on + strand, within HMPREF1058_RS03285 at 314.652 kb on - strand, within HMPREF1058_RS03285 at 314.652 kb on - strand, within HMPREF1058_RS03285 at 314.657 kb on + strand at 314.668 kb on - strand, within HMPREF1058_RS03285 at 314.829 kb on - strand, within HMPREF1058_RS03285 at 314.865 kb on + strand, within HMPREF1058_RS03285 at 314.865 kb on + strand, within HMPREF1058_RS03285 at 314.866 kb on - strand, within HMPREF1058_RS03285 at 314.866 kb on - strand, within HMPREF1058_RS03285 at 315.000 kb on - strand, within HMPREF1058_RS03285 at 315.003 kb on - strand, within HMPREF1058_RS03285 at 315.075 kb on + strand, within HMPREF1058_RS03285 at 315.117 kb on - strand, within HMPREF1058_RS03285 at 315.168 kb on - strand at 315.224 kb on + strand at 315.239 kb on + strand at 315.293 kb on + strand at 315.294 kb on - strand at 315.316 kb on - strand at 315.326 kb on - strand at 315.364 kb on + strand at 315.407 kb on + strand at 315.495 kb on - strand, within HMPREF1058_RS03280 at 315.682 kb on - strand, within HMPREF1058_RS03280 at 315.685 kb on + strand, within HMPREF1058_RS03280 at 315.763 kb on + strand, within HMPREF1058_RS03280 at 315.781 kb on + strand, within HMPREF1058_RS03280 at 315.782 kb on - strand, within HMPREF1058_RS03280 at 315.782 kb on - strand, within HMPREF1058_RS03280 at 315.818 kb on + strand, within HMPREF1058_RS03280 at 316.020 kb on - strand, within HMPREF1058_RS03280 at 316.028 kb on + strand, within HMPREF1058_RS03280 at 316.029 kb on - strand, within HMPREF1058_RS03280 at 316.032 kb on + strand, within HMPREF1058_RS03280 at 316.087 kb on - strand, within HMPREF1058_RS03280 at 316.095 kb on - strand, within HMPREF1058_RS03280 at 316.129 kb on - strand, within HMPREF1058_RS03280
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 313,461 + -3.6 313,462 - -3.4 313,505 + -3.7 313,507 + +1.2 313,507 + -0.8 313,550 + +0.1 313,618 + +0.1 313,619 - -1.9 313,620 + -3.1 313,621 - -4.9 313,621 - +0.8 313,623 + -0.4 313,659 - HMPREF1058_RS03295 0.17 -1.9 313,754 + HMPREF1058_RS03295 0.65 -3.5 313,865 + +1.8 313,866 - -1.9 313,866 - -5.2 313,866 - -2.7 313,871 + -1.4 313,875 - -2.1 313,924 - HMPREF1058_RS03290 0.18 -0.7 314,106 + HMPREF1058_RS03290 0.47 -1.3 314,107 - HMPREF1058_RS03290 0.47 -2.9 314,155 - HMPREF1058_RS03290 0.54 -3.2 314,183 + HMPREF1058_RS03290 0.59 -1.3 314,184 - HMPREF1058_RS03290 0.59 -1.2 314,388 + -0.0 314,389 - -5.0 314,441 + +0.6 314,455 - -3.3 314,455 - -4.6 314,475 - -3.8 314,490 + -2.2 314,623 - HMPREF1058_RS03285 0.21 -0.2 314,634 + HMPREF1058_RS03285 0.23 -4.0 314,651 + HMPREF1058_RS03285 0.25 -2.7 314,652 - HMPREF1058_RS03285 0.25 -2.8 314,652 - HMPREF1058_RS03285 0.25 -1.4 314,657 + -0.4 314,668 - HMPREF1058_RS03285 0.27 -3.0 314,829 - HMPREF1058_RS03285 0.48 -4.3 314,865 + HMPREF1058_RS03285 0.53 -1.3 314,865 + HMPREF1058_RS03285 0.53 -2.3 314,866 - HMPREF1058_RS03285 0.53 -3.3 314,866 - HMPREF1058_RS03285 0.53 -2.8 315,000 - HMPREF1058_RS03285 0.70 -0.7 315,003 - HMPREF1058_RS03285 0.70 -3.4 315,075 + HMPREF1058_RS03285 0.80 -2.4 315,117 - HMPREF1058_RS03285 0.85 -4.2 315,168 - -3.3 315,224 + -2.2 315,239 + -1.6 315,293 + -1.6 315,294 - -4.3 315,316 - -5.4 315,326 - -2.0 315,364 + -3.5 315,407 + -3.5 315,495 - HMPREF1058_RS03280 0.15 -5.2 315,682 - HMPREF1058_RS03280 0.26 -0.6 315,685 + HMPREF1058_RS03280 0.26 -1.3 315,763 + HMPREF1058_RS03280 0.31 -3.3 315,781 + HMPREF1058_RS03280 0.32 -4.0 315,782 - HMPREF1058_RS03280 0.32 -0.7 315,782 - HMPREF1058_RS03280 0.32 -3.7 315,818 + HMPREF1058_RS03280 0.34 -3.8 316,020 - HMPREF1058_RS03280 0.46 -0.0 316,028 + HMPREF1058_RS03280 0.46 -2.1 316,029 - HMPREF1058_RS03280 0.46 -3.3 316,032 + HMPREF1058_RS03280 0.47 -3.6 316,087 - HMPREF1058_RS03280 0.50 -1.6 316,095 - HMPREF1058_RS03280 0.50 -2.0 316,129 - HMPREF1058_RS03280 0.52 -2.7
Or see this region's nucleotide sequence