Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS03290 and HMPREF1058_RS03285 are separated by 17 nucleotides HMPREF1058_RS03285 and HMPREF1058_RS03280 are separated by 8 nucleotides HMPREF1058_RS03280 and HMPREF1058_RS03275 are separated by 21 nucleotides HMPREF1058_RS03275 and HMPREF1058_RS03270 overlap by 1 nucleotides
HMPREF1058_RS03290: HMPREF1058_RS03290 - thiamine phosphate synthase, at 313,814 to 314,440
_RS03290
HMPREF1058_RS03285: HMPREF1058_RS03285 - thiazole synthase, at 314,458 to 315,231
_RS03285
HMPREF1058_RS03280: HMPREF1058_RS03280 - phosphomethylpyrimidine synthase ThiC, at 315,240 to 316,940
_RS03280
HMPREF1058_RS03275: HMPREF1058_RS03275 - hypothetical protein, at 316,962 to 317,477
_RS03275
HMPREF1058_RS03270: HMPREF1058_RS03270 - flavodoxin family protein, at 317,477 to 318,049
_RS03270
Position (kb) 315
316
317 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 314.388 kb on + strand at 314.389 kb on - strand at 314.441 kb on + strand at 314.455 kb on - strand at 314.455 kb on - strand at 314.475 kb on - strand at 314.490 kb on + strand at 314.623 kb on - strand, within HMPREF1058_RS03285 at 314.634 kb on + strand, within HMPREF1058_RS03285 at 314.651 kb on + strand, within HMPREF1058_RS03285 at 314.652 kb on - strand, within HMPREF1058_RS03285 at 314.652 kb on - strand, within HMPREF1058_RS03285 at 314.657 kb on + strand at 314.668 kb on - strand, within HMPREF1058_RS03285 at 314.829 kb on - strand, within HMPREF1058_RS03285 at 314.865 kb on + strand, within HMPREF1058_RS03285 at 314.865 kb on + strand, within HMPREF1058_RS03285 at 314.866 kb on - strand, within HMPREF1058_RS03285 at 314.866 kb on - strand, within HMPREF1058_RS03285 at 315.000 kb on - strand, within HMPREF1058_RS03285 at 315.003 kb on - strand, within HMPREF1058_RS03285 at 315.075 kb on + strand, within HMPREF1058_RS03285 at 315.117 kb on - strand, within HMPREF1058_RS03285 at 315.168 kb on - strand at 315.224 kb on + strand at 315.239 kb on + strand at 315.293 kb on + strand at 315.294 kb on - strand at 315.316 kb on - strand at 315.326 kb on - strand at 315.364 kb on + strand at 315.407 kb on + strand at 315.495 kb on - strand, within HMPREF1058_RS03280 at 315.682 kb on - strand, within HMPREF1058_RS03280 at 315.685 kb on + strand, within HMPREF1058_RS03280 at 315.763 kb on + strand, within HMPREF1058_RS03280 at 315.781 kb on + strand, within HMPREF1058_RS03280 at 315.782 kb on - strand, within HMPREF1058_RS03280 at 315.782 kb on - strand, within HMPREF1058_RS03280 at 315.818 kb on + strand, within HMPREF1058_RS03280 at 316.020 kb on - strand, within HMPREF1058_RS03280 at 316.028 kb on + strand, within HMPREF1058_RS03280 at 316.029 kb on - strand, within HMPREF1058_RS03280 at 316.032 kb on + strand, within HMPREF1058_RS03280 at 316.087 kb on - strand, within HMPREF1058_RS03280 at 316.095 kb on - strand, within HMPREF1058_RS03280 at 316.129 kb on - strand, within HMPREF1058_RS03280 at 316.237 kb on + strand, within HMPREF1058_RS03280 at 316.258 kb on + strand, within HMPREF1058_RS03280 at 316.258 kb on + strand, within HMPREF1058_RS03280 at 316.260 kb on + strand, within HMPREF1058_RS03280 at 316.299 kb on + strand, within HMPREF1058_RS03280 at 316.299 kb on + strand, within HMPREF1058_RS03280 at 316.306 kb on + strand, within HMPREF1058_RS03280 at 316.403 kb on - strand, within HMPREF1058_RS03280 at 316.456 kb on + strand, within HMPREF1058_RS03280 at 316.457 kb on - strand, within HMPREF1058_RS03280 at 316.457 kb on - strand, within HMPREF1058_RS03280 at 316.467 kb on - strand, within HMPREF1058_RS03280 at 316.626 kb on + strand, within HMPREF1058_RS03280 at 316.626 kb on + strand, within HMPREF1058_RS03280 at 316.627 kb on - strand, within HMPREF1058_RS03280 at 316.627 kb on - strand, within HMPREF1058_RS03280 at 316.672 kb on + strand, within HMPREF1058_RS03280 at 316.673 kb on - strand, within HMPREF1058_RS03280 at 316.673 kb on - strand, within HMPREF1058_RS03280 at 316.673 kb on - strand, within HMPREF1058_RS03280 at 316.673 kb on - strand, within HMPREF1058_RS03280 at 316.679 kb on - strand, within HMPREF1058_RS03280 at 316.762 kb on + strand, within HMPREF1058_RS03280 at 316.870 kb on - strand at 317.011 kb on + strand at 317.012 kb on - strand at 317.020 kb on + strand, within HMPREF1058_RS03275 at 317.021 kb on - strand, within HMPREF1058_RS03275 at 317.350 kb on - strand, within HMPREF1058_RS03275 at 317.421 kb on + strand, within HMPREF1058_RS03275 at 317.425 kb on - strand, within HMPREF1058_RS03275 at 317.476 kb on - strand at 317.484 kb on + strand at 317.601 kb on - strand, within HMPREF1058_RS03270 at 317.694 kb on + strand, within HMPREF1058_RS03270 at 317.764 kb on - strand, within HMPREF1058_RS03270 at 317.884 kb on + strand, within HMPREF1058_RS03270 at 317.914 kb on - strand, within HMPREF1058_RS03270
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 314,388 + -0.0 314,389 - -5.0 314,441 + +0.6 314,455 - -3.3 314,455 - -4.6 314,475 - -3.8 314,490 + -2.2 314,623 - HMPREF1058_RS03285 0.21 -0.2 314,634 + HMPREF1058_RS03285 0.23 -4.0 314,651 + HMPREF1058_RS03285 0.25 -2.7 314,652 - HMPREF1058_RS03285 0.25 -2.8 314,652 - HMPREF1058_RS03285 0.25 -1.4 314,657 + -0.4 314,668 - HMPREF1058_RS03285 0.27 -3.0 314,829 - HMPREF1058_RS03285 0.48 -4.3 314,865 + HMPREF1058_RS03285 0.53 -1.3 314,865 + HMPREF1058_RS03285 0.53 -2.3 314,866 - HMPREF1058_RS03285 0.53 -3.3 314,866 - HMPREF1058_RS03285 0.53 -2.8 315,000 - HMPREF1058_RS03285 0.70 -0.7 315,003 - HMPREF1058_RS03285 0.70 -3.4 315,075 + HMPREF1058_RS03285 0.80 -2.4 315,117 - HMPREF1058_RS03285 0.85 -4.2 315,168 - -3.3 315,224 + -2.2 315,239 + -1.6 315,293 + -1.6 315,294 - -4.3 315,316 - -5.4 315,326 - -2.0 315,364 + -3.5 315,407 + -3.5 315,495 - HMPREF1058_RS03280 0.15 -5.2 315,682 - HMPREF1058_RS03280 0.26 -0.6 315,685 + HMPREF1058_RS03280 0.26 -1.3 315,763 + HMPREF1058_RS03280 0.31 -3.3 315,781 + HMPREF1058_RS03280 0.32 -4.0 315,782 - HMPREF1058_RS03280 0.32 -0.7 315,782 - HMPREF1058_RS03280 0.32 -3.7 315,818 + HMPREF1058_RS03280 0.34 -3.8 316,020 - HMPREF1058_RS03280 0.46 -0.0 316,028 + HMPREF1058_RS03280 0.46 -2.1 316,029 - HMPREF1058_RS03280 0.46 -3.3 316,032 + HMPREF1058_RS03280 0.47 -3.6 316,087 - HMPREF1058_RS03280 0.50 -1.6 316,095 - HMPREF1058_RS03280 0.50 -2.0 316,129 - HMPREF1058_RS03280 0.52 -2.7 316,237 + HMPREF1058_RS03280 0.59 -0.4 316,258 + HMPREF1058_RS03280 0.60 -3.0 316,258 + HMPREF1058_RS03280 0.60 -2.0 316,260 + HMPREF1058_RS03280 0.60 -2.2 316,299 + HMPREF1058_RS03280 0.62 -3.7 316,299 + HMPREF1058_RS03280 0.62 -5.0 316,306 + HMPREF1058_RS03280 0.63 -1.6 316,403 - HMPREF1058_RS03280 0.68 -3.5 316,456 + HMPREF1058_RS03280 0.71 -2.6 316,457 - HMPREF1058_RS03280 0.72 -2.9 316,457 - HMPREF1058_RS03280 0.72 -2.8 316,467 - HMPREF1058_RS03280 0.72 -4.8 316,626 + HMPREF1058_RS03280 0.81 -0.2 316,626 + HMPREF1058_RS03280 0.81 -4.1 316,627 - HMPREF1058_RS03280 0.82 -3.0 316,627 - HMPREF1058_RS03280 0.82 -2.4 316,672 + HMPREF1058_RS03280 0.84 -2.8 316,673 - HMPREF1058_RS03280 0.84 -4.8 316,673 - HMPREF1058_RS03280 0.84 +1.8 316,673 - HMPREF1058_RS03280 0.84 -1.6 316,673 - HMPREF1058_RS03280 0.84 -2.6 316,679 - HMPREF1058_RS03280 0.85 -1.3 316,762 + HMPREF1058_RS03280 0.89 -2.0 316,870 - -2.5 317,011 + +0.7 317,012 - -1.9 317,020 + HMPREF1058_RS03275 0.11 +1.3 317,021 - HMPREF1058_RS03275 0.11 -1.8 317,350 - HMPREF1058_RS03275 0.75 -2.4 317,421 + HMPREF1058_RS03275 0.89 +1.0 317,425 - HMPREF1058_RS03275 0.90 -1.4 317,476 - -1.1 317,484 + -1.1 317,601 - HMPREF1058_RS03270 0.22 -2.3 317,694 + HMPREF1058_RS03270 0.38 -0.1 317,764 - HMPREF1058_RS03270 0.50 -3.8 317,884 + HMPREF1058_RS03270 0.71 +0.2 317,914 - HMPREF1058_RS03270 0.76 -3.4
Or see this region's nucleotide sequence