Experiment: D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS02625 and HMPREF1058_RS02620 are separated by 63 nucleotides HMPREF1058_RS02620 and HMPREF1058_RS02615 are separated by 12 nucleotides
HMPREF1058_RS02625: HMPREF1058_RS02625 - [FeFe] hydrogenase H-cluster maturation GTPase HydF, at 139,829 to 141,019
_RS02625
HMPREF1058_RS02620: HMPREF1058_RS02620 - [FeFe] hydrogenase H-cluster radical SAM maturase HydG, at 141,083 to 142,501
_RS02620
HMPREF1058_RS02615: HMPREF1058_RS02615 - [FeFe] hydrogenase H-cluster radical SAM maturase HydE, at 142,514 to 143,548
_RS02615
Position (kb)
141
142
143 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 140.176 kb on - strand, within HMPREF1058_RS02625 at 140.178 kb on + strand, within HMPREF1058_RS02625 at 140.179 kb on - strand, within HMPREF1058_RS02625 at 140.179 kb on - strand, within HMPREF1058_RS02625 at 140.184 kb on - strand, within HMPREF1058_RS02625 at 140.184 kb on - strand, within HMPREF1058_RS02625 at 140.341 kb on + strand, within HMPREF1058_RS02625 at 140.373 kb on - strand, within HMPREF1058_RS02625 at 140.390 kb on + strand, within HMPREF1058_RS02625 at 140.390 kb on + strand, within HMPREF1058_RS02625 at 140.427 kb on - strand, within HMPREF1058_RS02625 at 140.453 kb on + strand, within HMPREF1058_RS02625 at 140.600 kb on + strand, within HMPREF1058_RS02625 at 140.601 kb on - strand, within HMPREF1058_RS02625 at 140.621 kb on + strand, within HMPREF1058_RS02625 at 140.771 kb on - strand, within HMPREF1058_RS02625 at 140.846 kb on - strand, within HMPREF1058_RS02625 at 140.980 kb on + strand at 140.981 kb on - strand at 140.984 kb on + strand at 141.012 kb on - strand at 141.020 kb on - strand at 141.113 kb on + strand at 141.163 kb on + strand at 141.163 kb on + strand at 141.305 kb on - strand, within HMPREF1058_RS02620 at 141.305 kb on - strand, within HMPREF1058_RS02620 at 141.320 kb on - strand, within HMPREF1058_RS02620 at 141.547 kb on + strand, within HMPREF1058_RS02620 at 141.553 kb on + strand, within HMPREF1058_RS02620 at 141.554 kb on - strand, within HMPREF1058_RS02620 at 141.554 kb on - strand, within HMPREF1058_RS02620 at 141.559 kb on + strand, within HMPREF1058_RS02620 at 141.609 kb on - strand, within HMPREF1058_RS02620 at 141.610 kb on + strand, within HMPREF1058_RS02620 at 141.685 kb on + strand, within HMPREF1058_RS02620 at 141.789 kb on - strand, within HMPREF1058_RS02620 at 141.837 kb on + strand, within HMPREF1058_RS02620 at 141.844 kb on - strand, within HMPREF1058_RS02620 at 141.844 kb on - strand, within HMPREF1058_RS02620 at 141.879 kb on + strand, within HMPREF1058_RS02620 at 141.914 kb on - strand, within HMPREF1058_RS02620 at 141.916 kb on - strand, within HMPREF1058_RS02620 at 141.916 kb on - strand, within HMPREF1058_RS02620 at 142.019 kb on - strand, within HMPREF1058_RS02620 at 142.020 kb on + strand, within HMPREF1058_RS02620 at 142.020 kb on + strand, within HMPREF1058_RS02620 at 142.020 kb on + strand, within HMPREF1058_RS02620 at 142.048 kb on - strand, within HMPREF1058_RS02620 at 142.054 kb on + strand at 142.054 kb on + strand, within HMPREF1058_RS02620 at 142.197 kb on + strand, within HMPREF1058_RS02620 at 142.247 kb on + strand, within HMPREF1058_RS02620 at 142.431 kb on + strand at 142.432 kb on - strand at 142.479 kb on - strand at 142.498 kb on - strand at 142.498 kb on - strand at 142.498 kb on - strand at 142.626 kb on + strand, within HMPREF1058_RS02615 at 142.627 kb on - strand, within HMPREF1058_RS02615 at 142.627 kb on - strand, within HMPREF1058_RS02615 at 142.677 kb on - strand, within HMPREF1058_RS02615 at 143.013 kb on - strand, within HMPREF1058_RS02615 at 143.041 kb on + strand, within HMPREF1058_RS02615 at 143.042 kb on - strand, within HMPREF1058_RS02615 at 143.448 kb on - strand at 143.451 kb on + strand at 143.451 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose-6-Phosphate 10 mM (C) mhCom Varel_Bryant_medium_lowCys Passage4 remove 140,176 - HMPREF1058_RS02625 0.29 -4.4 140,178 + HMPREF1058_RS02625 0.29 -3.1 140,179 - HMPREF1058_RS02625 0.29 -0.3 140,179 - HMPREF1058_RS02625 0.29 -3.5 140,184 - HMPREF1058_RS02625 0.30 -5.4 140,184 - HMPREF1058_RS02625 0.30 -3.2 140,341 + HMPREF1058_RS02625 0.43 -5.1 140,373 - HMPREF1058_RS02625 0.46 -4.8 140,390 + HMPREF1058_RS02625 0.47 -3.7 140,390 + HMPREF1058_RS02625 0.47 -2.9 140,427 - HMPREF1058_RS02625 0.50 -2.0 140,453 + HMPREF1058_RS02625 0.52 -2.3 140,600 + HMPREF1058_RS02625 0.65 -3.3 140,601 - HMPREF1058_RS02625 0.65 -3.0 140,621 + HMPREF1058_RS02625 0.66 -2.1 140,771 - HMPREF1058_RS02625 0.79 -4.3 140,846 - HMPREF1058_RS02625 0.85 -4.2 140,980 + -3.2 140,981 - -1.4 140,984 + -2.1 141,012 - -5.1 141,020 - -1.3 141,113 + -2.8 141,163 + -0.9 141,163 + -0.3 141,305 - HMPREF1058_RS02620 0.16 -4.5 141,305 - HMPREF1058_RS02620 0.16 -3.7 141,320 - HMPREF1058_RS02620 0.17 -2.6 141,547 + HMPREF1058_RS02620 0.33 -2.4 141,553 + HMPREF1058_RS02620 0.33 -2.5 141,554 - HMPREF1058_RS02620 0.33 -1.7 141,554 - HMPREF1058_RS02620 0.33 -5.7 141,559 + HMPREF1058_RS02620 0.34 -1.5 141,609 - HMPREF1058_RS02620 0.37 -1.2 141,610 + HMPREF1058_RS02620 0.37 -3.5 141,685 + HMPREF1058_RS02620 0.42 -2.3 141,789 - HMPREF1058_RS02620 0.50 -4.0 141,837 + HMPREF1058_RS02620 0.53 -2.2 141,844 - HMPREF1058_RS02620 0.54 -0.8 141,844 - HMPREF1058_RS02620 0.54 -1.1 141,879 + HMPREF1058_RS02620 0.56 -2.5 141,914 - HMPREF1058_RS02620 0.59 -3.0 141,916 - HMPREF1058_RS02620 0.59 -3.1 141,916 - HMPREF1058_RS02620 0.59 -3.9 142,019 - HMPREF1058_RS02620 0.66 -3.7 142,020 + HMPREF1058_RS02620 0.66 -3.5 142,020 + HMPREF1058_RS02620 0.66 -0.8 142,020 + HMPREF1058_RS02620 0.66 -4.4 142,048 - HMPREF1058_RS02620 0.68 -5.3 142,054 + -0.3 142,054 + HMPREF1058_RS02620 0.68 -2.3 142,197 + HMPREF1058_RS02620 0.79 -3.5 142,247 + HMPREF1058_RS02620 0.82 -1.0 142,431 + -3.8 142,432 - -4.6 142,479 - -4.6 142,498 - -3.1 142,498 - +1.1 142,498 - -3.8 142,626 + HMPREF1058_RS02615 0.11 -3.5 142,627 - HMPREF1058_RS02615 0.11 -3.4 142,627 - HMPREF1058_RS02615 0.11 -2.7 142,677 - HMPREF1058_RS02615 0.16 -1.4 143,013 - HMPREF1058_RS02615 0.48 -4.3 143,041 + HMPREF1058_RS02615 0.51 -3.8 143,042 - HMPREF1058_RS02615 0.51 -0.9 143,448 - -2.6 143,451 + -5.2 143,451 + -3.3
Or see this region's nucleotide sequence