Experiment: PEG (Day 3) - Cage2;Mouse1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3973 and tRNA-Pro are separated by 55 nucleotides tRNA-Pro and BT3974 are separated by 364 nucleotides BT3974 and BT3975 are separated by 123 nucleotides
BT3973: BT3973 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 5,173,216 to 5,174,436
BT3973
BT_t64: tRNA-Pro - tRNA-Pro (RefSeq), at 5,174,492 to 5,174,568
tRNA-Pro
BT3974: BT3974 - conserved hypothetical protein (NCBI ptt file), at 5,174,933 to 5,175,727
BT3974
BT3975: BT3975 - 3-dehydroquinate synthase (NCBI ptt file), at 5,175,851 to 5,176,912
BT3975
Position (kb)
5174
5175
5176 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 5173.960 kb on + strand, within BT3973 at 5174.050 kb on + strand, within BT3973 at 5174.357 kb on - strand at 5174.725 kb on - strand at 5174.747 kb on + strand at 5174.811 kb on + strand at 5174.870 kb on - strand at 5174.873 kb on - strand at 5174.897 kb on + strand at 5174.966 kb on - strand at 5174.968 kb on + strand at 5175.017 kb on - strand, within BT3974 at 5175.021 kb on + strand, within BT3974 at 5175.121 kb on + strand, within BT3974 at 5175.122 kb on - strand, within BT3974 at 5175.200 kb on - strand, within BT3974 at 5175.277 kb on + strand, within BT3974 at 5175.296 kb on - strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.298 kb on - strand, within BT3974 at 5175.299 kb on + strand, within BT3974 at 5175.300 kb on - strand, within BT3974 at 5175.333 kb on - strand, within BT3974 at 5175.336 kb on + strand, within BT3974 at 5175.404 kb on - strand, within BT3974 at 5175.438 kb on + strand, within BT3974 at 5175.461 kb on - strand, within BT3974 at 5175.485 kb on - strand, within BT3974 at 5175.485 kb on - strand, within BT3974 at 5175.562 kb on - strand, within BT3974 at 5175.573 kb on + strand, within BT3974 at 5175.573 kb on + strand, within BT3974 at 5175.613 kb on + strand, within BT3974 at 5175.614 kb on - strand, within BT3974 at 5175.715 kb on + strand at 5175.716 kb on - strand at 5175.731 kb on - strand at 5175.831 kb on + strand at 5175.831 kb on + strand at 5175.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PEG (Day 3) - Cage2;Mouse1 remove 5,173,960 + BT3973 0.61 -1.5 5,174,050 + BT3973 0.68 +0.0 5,174,357 - -1.5 5,174,725 - +1.1 5,174,747 + -0.7 5,174,811 + -0.6 5,174,870 - +0.9 5,174,873 - +2.1 5,174,897 + +2.1 5,174,966 - +5.3 5,174,968 + +5.8 5,175,017 - BT3974 0.11 +3.1 5,175,021 + BT3974 0.11 +3.5 5,175,121 + BT3974 0.24 +1.8 5,175,122 - BT3974 0.24 -0.1 5,175,200 - BT3974 0.34 +2.7 5,175,277 + BT3974 0.43 +1.8 5,175,296 - BT3974 0.46 +4.7 5,175,297 + BT3974 0.46 +8.9 5,175,297 + BT3974 0.46 +2.8 5,175,297 + BT3974 0.46 +1.6 5,175,297 + BT3974 0.46 +2.3 5,175,298 - BT3974 0.46 +2.7 5,175,299 + BT3974 0.46 +2.5 5,175,300 - BT3974 0.46 +0.9 5,175,333 - BT3974 0.50 +3.4 5,175,336 + BT3974 0.51 +6.4 5,175,404 - BT3974 0.59 +2.7 5,175,438 + BT3974 0.64 +4.2 5,175,461 - BT3974 0.66 +1.9 5,175,485 - BT3974 0.69 -0.7 5,175,485 - BT3974 0.69 +1.6 5,175,562 - BT3974 0.79 +2.5 5,175,573 + BT3974 0.81 +4.8 5,175,573 + BT3974 0.81 +2.5 5,175,613 + BT3974 0.86 +0.7 5,175,614 - BT3974 0.86 +8.9 5,175,715 + +3.4 5,175,716 - +1.0 5,175,731 - +1.5 5,175,831 + -3.5 5,175,831 + -0.6 5,175,863 - -1.9
Or see this region's nucleotide sequence