Experiment: PEG (Day 3) - Cage2;Mouse1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2081 and BT2082 are separated by 90 nucleotides BT2082 and BT2083 are separated by 15 nucleotides BT2083 and BT2084 are separated by 158 nucleotides
BT2081: BT2081 - hypothetical protein (NCBI ptt file), at 2,607,666 to 2,608,751
BT2081
BT2082: BT2082 - hypothetical protein (NCBI ptt file), at 2,608,842 to 2,609,627
BT2082
BT2083: BT2083 - hypothetical protein (NCBI ptt file), at 2,609,643 to 2,610,206
BT2083
BT2084: BT2084 - chorismate synthase (NCBI ptt file), at 2,610,365 to 2,611,441
BT2084
Position (kb)
2608
2609
2610 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 2607.981 kb on + strand, within BT2081 at 2607.989 kb on - strand, within BT2081 at 2608.057 kb on - strand, within BT2081 at 2608.238 kb on + strand, within BT2081 at 2608.348 kb on + strand, within BT2081 at 2608.349 kb on - strand, within BT2081 at 2608.349 kb on - strand, within BT2081 at 2608.368 kb on + strand, within BT2081 at 2608.379 kb on + strand, within BT2081 at 2608.436 kb on - strand, within BT2081 at 2608.436 kb on - strand, within BT2081 at 2608.458 kb on + strand, within BT2081 at 2608.459 kb on - strand, within BT2081 at 2608.529 kb on - strand, within BT2081 at 2608.570 kb on - strand, within BT2081 at 2608.589 kb on + strand, within BT2081 at 2608.590 kb on - strand, within BT2081 at 2608.641 kb on - strand, within BT2081 at 2608.646 kb on - strand at 2608.703 kb on + strand at 2608.730 kb on - strand at 2608.782 kb on - strand at 2608.814 kb on + strand at 2608.819 kb on - strand at 2608.819 kb on - strand at 2608.857 kb on + strand at 2608.861 kb on + strand at 2609.169 kb on + strand, within BT2082 at 2609.171 kb on + strand, within BT2082 at 2609.359 kb on + strand, within BT2082 at 2609.362 kb on - strand, within BT2082 at 2609.370 kb on + strand, within BT2082 at 2609.411 kb on + strand, within BT2082 at 2609.421 kb on - strand, within BT2082 at 2609.434 kb on - strand, within BT2082 at 2609.479 kb on + strand, within BT2082 at 2609.479 kb on + strand, within BT2082 at 2609.480 kb on - strand, within BT2082 at 2609.480 kb on - strand, within BT2082 at 2609.513 kb on + strand, within BT2082 at 2609.513 kb on + strand, within BT2082 at 2609.513 kb on + strand, within BT2082 at 2609.514 kb on - strand, within BT2082 at 2609.514 kb on - strand, within BT2082 at 2609.514 kb on - strand, within BT2082 at 2609.517 kb on + strand, within BT2082 at 2609.517 kb on + strand, within BT2082 at 2609.518 kb on - strand, within BT2082 at 2609.518 kb on - strand, within BT2082 at 2609.546 kb on + strand, within BT2082 at 2609.546 kb on + strand, within BT2082 at 2609.546 kb on + strand, within BT2082 at 2609.547 kb on - strand, within BT2082 at 2609.547 kb on - strand, within BT2082 at 2609.574 kb on - strand at 2609.582 kb on + strand at 2609.626 kb on - strand at 2609.644 kb on + strand at 2609.739 kb on - strand, within BT2083 at 2609.739 kb on - strand, within BT2083 at 2609.767 kb on - strand, within BT2083 at 2609.813 kb on + strand, within BT2083 at 2609.878 kb on - strand, within BT2083 at 2610.172 kb on - strand at 2610.187 kb on + strand at 2610.187 kb on + strand at 2610.219 kb on + strand at 2610.219 kb on + strand at 2610.242 kb on - strand at 2610.313 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PEG (Day 3) - Cage2;Mouse1 remove 2,607,981 + BT2081 0.29 -1.6 2,607,989 - BT2081 0.30 -2.0 2,608,057 - BT2081 0.36 -1.6 2,608,238 + BT2081 0.53 -0.3 2,608,348 + BT2081 0.63 -0.1 2,608,349 - BT2081 0.63 -1.8 2,608,349 - BT2081 0.63 -1.7 2,608,368 + BT2081 0.65 -2.0 2,608,379 + BT2081 0.66 -2.2 2,608,436 - BT2081 0.71 -0.3 2,608,436 - BT2081 0.71 +1.4 2,608,458 + BT2081 0.73 -0.6 2,608,459 - BT2081 0.73 +0.3 2,608,529 - BT2081 0.79 -4.0 2,608,570 - BT2081 0.83 -2.5 2,608,589 + BT2081 0.85 +0.5 2,608,590 - BT2081 0.85 +0.4 2,608,641 - BT2081 0.90 -1.3 2,608,646 - -2.6 2,608,703 + -1.8 2,608,730 - -2.8 2,608,782 - -1.9 2,608,814 + -2.8 2,608,819 - -3.7 2,608,819 - +0.8 2,608,857 + -1.6 2,608,861 + -0.6 2,609,169 + BT2082 0.42 -0.5 2,609,171 + BT2082 0.42 +0.1 2,609,359 + BT2082 0.66 -0.9 2,609,362 - BT2082 0.66 -0.6 2,609,370 + BT2082 0.67 +0.1 2,609,411 + BT2082 0.72 +6.9 2,609,421 - BT2082 0.74 -3.3 2,609,434 - BT2082 0.75 +0.7 2,609,479 + BT2082 0.81 -0.9 2,609,479 + BT2082 0.81 -2.4 2,609,480 - BT2082 0.81 -0.4 2,609,480 - BT2082 0.81 -1.3 2,609,513 + BT2082 0.85 -1.1 2,609,513 + BT2082 0.85 -2.2 2,609,513 + BT2082 0.85 -2.3 2,609,514 - BT2082 0.85 +0.1 2,609,514 - BT2082 0.85 -0.3 2,609,514 - BT2082 0.85 +0.7 2,609,517 + BT2082 0.86 -2.0 2,609,517 + BT2082 0.86 +0.7 2,609,518 - BT2082 0.86 -1.3 2,609,518 - BT2082 0.86 +0.3 2,609,546 + BT2082 0.90 -0.6 2,609,546 + BT2082 0.90 -1.1 2,609,546 + BT2082 0.90 -0.9 2,609,547 - BT2082 0.90 -3.0 2,609,547 - BT2082 0.90 -0.7 2,609,574 - +0.7 2,609,582 + -1.6 2,609,626 - -0.9 2,609,644 + -0.4 2,609,739 - BT2083 0.17 -1.1 2,609,739 - BT2083 0.17 +0.8 2,609,767 - BT2083 0.22 -3.6 2,609,813 + BT2083 0.30 -2.6 2,609,878 - BT2083 0.42 +0.1 2,610,172 - -1.8 2,610,187 + +1.7 2,610,187 + -2.1 2,610,219 + -0.9 2,610,219 + -1.6 2,610,242 - -0.9 2,610,313 + -1.8
Or see this region's nucleotide sequence