Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27610 and BWI76_RS27615 are separated by 95 nucleotides BWI76_RS27615 and BWI76_RS27620 are separated by 185 nucleotides BWI76_RS27620 and BWI76_RS27625 are separated by 112 nucleotides
BWI76_RS27610: BWI76_RS27610 - hydroxyacid dehydrogenase, at 5,731,133 to 5,731,647
_RS27610
BWI76_RS27615: BWI76_RS27615 - putative Shikimate kinase, at 5,731,743 to 5,732,348
_RS27615
BWI76_RS27620: BWI76_RS27620 - hypothetical protein, at 5,732,534 to 5,733,001
_RS27620
BWI76_RS27625: BWI76_RS27625 - NADPH:quinone reductase, at 5,733,114 to 5,734,121
_RS27625
Position (kb) 5732
5733
5734 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5731.576 kb on - strand, within BWI76_RS27610 at 5731.627 kb on - strand at 5731.627 kb on - strand at 5731.650 kb on - strand at 5731.710 kb on + strand at 5731.718 kb on - strand at 5731.718 kb on - strand at 5731.727 kb on - strand at 5731.727 kb on - strand at 5731.852 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.989 kb on - strand, within BWI76_RS27615 at 5732.019 kb on + strand, within BWI76_RS27615 at 5732.153 kb on + strand, within BWI76_RS27615 at 5732.178 kb on - strand, within BWI76_RS27615 at 5732.280 kb on - strand, within BWI76_RS27615 at 5732.294 kb on + strand at 5732.348 kb on + strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.374 kb on + strand at 5732.432 kb on + strand at 5732.440 kb on - strand at 5732.460 kb on - strand at 5732.492 kb on - strand at 5732.568 kb on + strand at 5732.576 kb on - strand at 5732.576 kb on - strand at 5732.587 kb on + strand, within BWI76_RS27620 at 5732.624 kb on - strand, within BWI76_RS27620 at 5732.631 kb on + strand, within BWI76_RS27620 at 5732.639 kb on - strand, within BWI76_RS27620 at 5732.639 kb on - strand, within BWI76_RS27620 at 5732.925 kb on - strand, within BWI76_RS27620 at 5732.956 kb on - strand at 5733.066 kb on + strand at 5733.069 kb on - strand at 5733.070 kb on - strand at 5733.112 kb on + strand at 5733.389 kb on + strand, within BWI76_RS27625 at 5733.469 kb on - strand, within BWI76_RS27625 at 5733.532 kb on + strand, within BWI76_RS27625 at 5733.830 kb on + strand, within BWI76_RS27625 at 5733.870 kb on - strand, within BWI76_RS27625 at 5733.905 kb on - strand, within BWI76_RS27625 at 5733.924 kb on + strand, within BWI76_RS27625
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 5,731,576 - BWI76_RS27610 0.86 +0.2 5,731,627 - +1.1 5,731,627 - +0.6 5,731,650 - +0.4 5,731,710 + +0.4 5,731,718 - +1.1 5,731,718 - -1.0 5,731,727 - -0.0 5,731,727 - -0.5 5,731,852 + BWI76_RS27615 0.18 +0.8 5,731,952 + BWI76_RS27615 0.34 -1.2 5,731,952 + BWI76_RS27615 0.34 +0.9 5,731,952 + BWI76_RS27615 0.34 +1.6 5,731,960 - BWI76_RS27615 0.36 +0.4 5,731,960 - BWI76_RS27615 0.36 -0.3 5,731,960 - BWI76_RS27615 0.36 +0.1 5,731,960 - BWI76_RS27615 0.36 +0.9 5,731,989 - BWI76_RS27615 0.41 -2.1 5,732,019 + BWI76_RS27615 0.46 +2.1 5,732,153 + BWI76_RS27615 0.68 +1.3 5,732,178 - BWI76_RS27615 0.72 +0.8 5,732,280 - BWI76_RS27615 0.89 -2.0 5,732,294 + +0.1 5,732,348 + +0.0 5,732,356 - +1.4 5,732,356 - +0.3 5,732,356 - -0.2 5,732,356 - +0.4 5,732,374 + -0.1 5,732,432 + +1.4 5,732,440 - -0.3 5,732,460 - +3.7 5,732,492 - -1.2 5,732,568 + +0.3 5,732,576 - -0.1 5,732,576 - +3.6 5,732,587 + BWI76_RS27620 0.11 +0.1 5,732,624 - BWI76_RS27620 0.19 -0.1 5,732,631 + BWI76_RS27620 0.21 +0.4 5,732,639 - BWI76_RS27620 0.22 -2.6 5,732,639 - BWI76_RS27620 0.22 -2.5 5,732,925 - BWI76_RS27620 0.84 -2.9 5,732,956 - +0.6 5,733,066 + +0.3 5,733,069 - -1.3 5,733,070 - +0.5 5,733,112 + -0.4 5,733,389 + BWI76_RS27625 0.27 +0.7 5,733,469 - BWI76_RS27625 0.35 +0.7 5,733,532 + BWI76_RS27625 0.41 +1.5 5,733,830 + BWI76_RS27625 0.71 -0.3 5,733,870 - BWI76_RS27625 0.75 -0.9 5,733,905 - BWI76_RS27625 0.78 +0.5 5,733,924 + BWI76_RS27625 0.80 -0.2
Or see this region's nucleotide sequence