Experiment: L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26485 and BWI76_RS26490 are separated by 401 nucleotides BWI76_RS26490 and BWI76_RS26495 are separated by 100 nucleotides
BWI76_RS26485: BWI76_RS26485 - UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase, at 5,481,595 to 5,482,734
_RS26485
BWI76_RS26490: BWI76_RS26490 - phenolic acid decarboxylase, at 5,483,136 to 5,483,639
_RS26490
BWI76_RS26495: BWI76_RS26495 - LysR family transcriptional regulator, at 5,483,740 to 5,484,600
_RS26495
Position (kb)
5483
5484 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5482.329 kb on + strand, within BWI76_RS26485 at 5482.348 kb on + strand, within BWI76_RS26485 at 5482.489 kb on + strand, within BWI76_RS26485 at 5482.529 kb on - strand, within BWI76_RS26485 at 5482.581 kb on - strand, within BWI76_RS26485 at 5482.656 kb on - strand at 5482.667 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.686 kb on + strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.694 kb on - strand at 5482.696 kb on + strand at 5482.704 kb on - strand at 5482.704 kb on - strand at 5482.738 kb on - strand at 5482.739 kb on + strand at 5482.749 kb on + strand at 5482.749 kb on + strand at 5482.749 kb on + strand at 5482.757 kb on - strand at 5482.757 kb on - strand at 5482.786 kb on + strand at 5482.800 kb on + strand at 5482.803 kb on - strand at 5482.803 kb on - strand at 5482.810 kb on - strand at 5482.831 kb on - strand at 5482.885 kb on + strand at 5482.885 kb on + strand at 5482.885 kb on + strand at 5482.885 kb on + strand at 5482.895 kb on + strand at 5482.895 kb on + strand at 5482.895 kb on + strand at 5482.903 kb on - strand at 5482.995 kb on + strand at 5482.995 kb on - strand at 5482.995 kb on - strand at 5482.995 kb on - strand at 5483.035 kb on + strand at 5483.134 kb on + strand at 5483.134 kb on + strand at 5483.135 kb on + strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.142 kb on - strand at 5483.174 kb on - strand at 5483.181 kb on + strand at 5483.188 kb on + strand, within BWI76_RS26490 at 5483.193 kb on + strand, within BWI76_RS26490 at 5483.201 kb on - strand, within BWI76_RS26490 at 5483.201 kb on - strand, within BWI76_RS26490 at 5483.206 kb on + strand, within BWI76_RS26490 at 5483.223 kb on + strand, within BWI76_RS26490 at 5483.230 kb on - strand, within BWI76_RS26490 at 5483.270 kb on - strand, within BWI76_RS26490 at 5483.332 kb on + strand, within BWI76_RS26490 at 5483.408 kb on + strand, within BWI76_RS26490 at 5483.421 kb on - strand, within BWI76_RS26490 at 5483.465 kb on + strand at 5483.467 kb on + strand, within BWI76_RS26490 at 5483.473 kb on - strand at 5483.475 kb on - strand, within BWI76_RS26490 at 5483.525 kb on + strand, within BWI76_RS26490 at 5483.526 kb on + strand, within BWI76_RS26490 at 5483.594 kb on - strand at 5483.615 kb on + strand at 5483.623 kb on - strand at 5483.650 kb on + strand at 5483.879 kb on + strand, within BWI76_RS26495 at 5483.914 kb on + strand, within BWI76_RS26495 at 5483.914 kb on + strand, within BWI76_RS26495 at 5483.949 kb on + strand, within BWI76_RS26495 at 5483.957 kb on - strand, within BWI76_RS26495 at 5483.993 kb on + strand, within BWI76_RS26495 at 5484.001 kb on - strand, within BWI76_RS26495 at 5484.083 kb on + strand, within BWI76_RS26495 at 5484.417 kb on + strand, within BWI76_RS26495 at 5484.585 kb on + strand at 5484.585 kb on + strand at 5484.616 kb on - strand at 5484.616 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days remove 5,482,329 + BWI76_RS26485 0.64 +0.2 5,482,348 + BWI76_RS26485 0.66 +1.0 5,482,489 + BWI76_RS26485 0.78 -2.2 5,482,529 - BWI76_RS26485 0.82 -0.9 5,482,581 - BWI76_RS26485 0.86 -0.3 5,482,656 - +1.7 5,482,667 + +0.9 5,482,686 + +0.8 5,482,686 + -0.5 5,482,686 + +1.3 5,482,686 + +0.3 5,482,686 + +0.8 5,482,686 + -0.1 5,482,686 + -0.3 5,482,686 + -0.8 5,482,686 + -0.7 5,482,686 + -2.6 5,482,686 + +0.4 5,482,686 + +0.7 5,482,686 + -0.3 5,482,686 + +0.3 5,482,686 + -0.2 5,482,686 + -0.2 5,482,686 + +0.7 5,482,686 + +2.4 5,482,686 + +1.6 5,482,686 + -0.6 5,482,686 + +0.8 5,482,686 + -0.1 5,482,686 + -0.1 5,482,694 - -1.3 5,482,694 - +1.3 5,482,694 - +2.2 5,482,694 - -0.1 5,482,694 - -0.9 5,482,694 - +1.5 5,482,694 - -0.4 5,482,694 - +0.6 5,482,694 - -0.7 5,482,694 - +1.9 5,482,694 - +0.5 5,482,694 - +0.6 5,482,694 - +0.4 5,482,694 - +0.0 5,482,694 - -4.1 5,482,694 - +0.1 5,482,694 - +1.0 5,482,694 - -0.1 5,482,694 - -0.2 5,482,694 - -0.5 5,482,694 - +1.3 5,482,694 - -0.4 5,482,694 - -0.6 5,482,694 - +0.9 5,482,694 - +1.3 5,482,694 - +0.3 5,482,694 - -0.2 5,482,696 + +0.4 5,482,704 - +1.9 5,482,704 - -0.2 5,482,738 - -0.1 5,482,739 + -0.2 5,482,749 + -0.4 5,482,749 + +0.3 5,482,749 + -0.0 5,482,757 - -0.5 5,482,757 - +0.7 5,482,786 + -0.8 5,482,800 + -0.0 5,482,803 - +0.4 5,482,803 - -0.1 5,482,810 - +1.0 5,482,831 - -0.2 5,482,885 + +2.0 5,482,885 + +1.3 5,482,885 + -0.1 5,482,885 + +0.1 5,482,895 + +0.4 5,482,895 + -1.0 5,482,895 + +1.8 5,482,903 - +0.4 5,482,995 + +0.1 5,482,995 - -0.7 5,482,995 - +0.7 5,482,995 - -0.9 5,483,035 + -0.0 5,483,134 + +2.2 5,483,134 + -0.2 5,483,135 + +0.4 5,483,142 - -0.0 5,483,142 - -2.4 5,483,142 - +0.2 5,483,142 - -0.0 5,483,142 - -0.2 5,483,142 - +0.4 5,483,174 - +0.6 5,483,181 + -0.1 5,483,188 + BWI76_RS26490 0.10 +0.8 5,483,193 + BWI76_RS26490 0.11 +0.7 5,483,201 - BWI76_RS26490 0.13 +0.1 5,483,201 - BWI76_RS26490 0.13 +0.0 5,483,206 + BWI76_RS26490 0.14 -0.8 5,483,223 + BWI76_RS26490 0.17 -1.6 5,483,230 - BWI76_RS26490 0.19 -1.1 5,483,270 - BWI76_RS26490 0.27 -1.0 5,483,332 + BWI76_RS26490 0.39 -0.9 5,483,408 + BWI76_RS26490 0.54 +0.4 5,483,421 - BWI76_RS26490 0.57 -0.2 5,483,465 + +1.6 5,483,467 + BWI76_RS26490 0.66 -0.6 5,483,473 - +0.0 5,483,475 - BWI76_RS26490 0.67 -0.7 5,483,525 + BWI76_RS26490 0.77 +0.2 5,483,526 + BWI76_RS26490 0.77 -0.4 5,483,594 - +1.7 5,483,615 + -0.2 5,483,623 - +0.3 5,483,650 + +0.0 5,483,879 + BWI76_RS26495 0.16 +0.5 5,483,914 + BWI76_RS26495 0.20 +0.0 5,483,914 + BWI76_RS26495 0.20 -0.3 5,483,949 + BWI76_RS26495 0.24 -0.3 5,483,957 - BWI76_RS26495 0.25 +1.4 5,483,993 + BWI76_RS26495 0.29 +0.4 5,484,001 - BWI76_RS26495 0.30 -0.3 5,484,083 + BWI76_RS26495 0.40 +0.6 5,484,417 + BWI76_RS26495 0.79 -0.0 5,484,585 + +0.7 5,484,585 + +1.3 5,484,616 - -0.8 5,484,616 - -0.5
Or see this region's nucleotide sequence